Effect of flaxseed Lignans concentrate on oxidative stress in Caenorhabditis elegans (original) (raw)
This experiment was carried out to determine the effect of the flaxseed lignans concentrate (LC) on the lifespan and oxidative status of C. elegans under oxidative stress. Lifespan was determined by counting the number of surviving nematodes daily under a Lumascope microscope camera after treatment with LC. The evaluated oxidative markers included lipofuscin, which was measured using a fluorescent microscope. In our study, 0.2 mg/ml LC was found to be the optimum concentration to increase the mean lifespan of C. elegans, The accumulation of the age marker lipofuscin, which increased with hydrogen peroxide exposure, was decreased with upon treatment with LC. The LC enhanced the lifespan C. elegans and reduced the accumulation of lipofuscin.
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