Sex Chromosome Alignment at Meiosis of Azoospermic Men With Azoospermia Factor Microdeletion (original) (raw)

Journal of Andrology, 2004

Abstract

ABSTRACT: Deletions in the q arm of the Y chromosome result in spermatogenesis impairment. The aim of the present study was to observe the × and Y chromosome alignment in the spermatocytes of men with Y chromosome microdeletion of the azoospermia factor (AZF) region. This was performed by multicolor fluorescence in situ hybridization probes for the centromere and telomere regions. Testicular biopsies were performed in a testicular sperm extraction‐intracytoplasmic sperm injection set‐up in 11 azoospermic men: 8 (nonobstructive) with AZF deletions and 3 (obstructive) controls. Histological sections, cytology preparations of the testicular biopsies, and evaluation of the meiosis according to the percentage of XY and 18 bivalents formation were assessed. Spermatozoa were identified in at least one location in controls and specimens with AZFc‐deleted Y chromosomes. Complete spermatocyte arrest was found in those with a deletion that included the entire AZFb region. Bivalent formation ra...

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