Serotyping of European isolates of Chlamydia psittaci from poultry and other birds (original) (raw)
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Veterinary Microbiology, 2013
Please cite this article as: Yin, L., Kalmar, I., Lagae, S., Vandendriessche, S., Vanderhaeghen, W., Butaye, P., Cox, E., Vanrompay, D., Emerging Chlamydia psittaci infections in the chicken industry and pathology of Chlamydia psittaci genotype B and D strains in specific pathogen free chickens, Veterinary Microbiology (2010), Abstract 26 Sera of 30 Belgian and 10 Northern French chicken farms were tested by a Chlamydia 27 (C.) psittaci MOMP-based ELISA. Ninety-six percent, 93% and 90% of the Belgian 28 broilers, broiler breeders and layers were seropositive. Ninety-one percent of the French 29 broilers were seropositive. In addition, tissues of 5 Belgian and 5 French broiler farms 30 were examined at slaughter. All French farms ware culture positive while C. psittaci was 31 cultured from the lungs of 80% of examined Belgian farms. Chlamydia psittaci infections 32 are apparently emerging in chickens raised in Belgium and Northern France. We could 33 proof Hill's-Evans' postulates for chicken-derived C. psittaci genotype B and D strains.
Revista Argentina De Microbiologia, 2017
In Argentina, the epidemiological and molecular characteristics of Chlamydia psittaci infections are still not sufficiently known. A total of 846 respiratory and 10 ocular samples from patients with suspected human psittacosis were tested for C. psittaci from January 2010 to March 2015. Four samples of birds related to these patients were also studied. Forty-eight samples were positive for C. psittaci by a nested PCR. The molecular characterization of twelve C. psittaci PCR-positive samples received in the National Reference Laboratory INEI-ANLIS ''Dr. Carlos G. Malbrán'', Buenos Aires, Argentina was performed. Eight positive samples from humans and four from birds were genotyped by ompA gene sequencing. C. psittaci genotype A was found in all human samples and in the related birds. This report contributes to our increasing knowledge of the epidemiological and molecular characteristics of C. psittaci to conduct effective surveillance of its zoonotic infections.
Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 1993
Several methods for detecting antibodies to Chlamydia psittaci using sera from pigeons were compared with regard to their sensitivity, specificity and efficacy. The reference method used was indirect immunofluorescence (IFI), which uses as antigen intracellular inclusions in McCoy cell monolayers. Indirect micro-immunofluorescence (MIF) using as antigen C. psittaci cultured on yolk sacs of embryonated eggs, direct and indirect complement fixation tests (CFT and ICFT, respectively), and enzyme-linked immunosorbent assay (ELISA) were compared to the reference method. Indirect micro-immunofluorescence proved to be the most efficient method, while ELISA was the most sensitive, though showing a very low specificity. No statistically significant difference was found in comparing the two complement fixation tests. The use of ICFT to check for the negative results obtained in CFT is questioned. Possible reasons for the different results with the methods used are discussed.
Emerging Chlamydia psittaci infections in chickens and examination of transmission to humans
Journal of Medical Microbiology, 2013
Chlamydia psittaci and atypical Chlamydiaceae infections are (re)-emerging in chickens. We therefore examined the prevalence of C. psittaci, atypical Chlamydiaceae and their zoonotic transmission on 19 Belgian chicken farms. Atypical Chlamydiaceae were not detected in chickens but 18 out of 19 farms were positive for C. psittaci by culture and PCR. C. psittaci ompA genotypes A and D were discovered. None of the examined humans (n = 31) was infected with atypical Chlamydiaceae, but 29 (93.5 %) of them were positive for C. psittaci by culture and PCR. Genotypes A, D and a mixed infection with genotypes C and D were found. Humans (n = 2) working at the C. psittaci-negative farm never had respiratory complaints, while 25 out of 29 positive farmers (86.2 %) reported yearly medical complaints potentially related to psittacosis. Four of them currently experienced respiratory disease and one of them was being treated with antibiotics. Four farmers (12.5 %) mentioned that they had pneumonia ...
This study was carried out on 68 randomly collected chickens located at Ras Sedr Research Station, Desert Research Center, 68 serum samples were examined serologically by complement fixation test (CFT). Twenty out of 68 (29.91%) had antibodies against Chlamydophila psittaci. Ten blood samples of the serologically positive cases were subjected to polymerase chain reaction (PCR) and showed positive results for Chlamydophila psittaci at 119 bp. Therefore PCR was found to be reliable, rapid, sensitive and specific technique for the detection Chlamydophila psittaci in birds. Serologically positive birds did not show any clinical symptoms of disease, but they were in contact with sheep and goat that showed previous abortion and were positive for C. abortus. It is recommended to avoid breeding of chickens with other animal species in the same yard because chickens become asymptomatic carrier with shedding of Chlamydophila psittaci in their feaces and respiratory discharges.
European Journal of Wildlife Research, 2009
To determine the prevalence of Chlamydophila psittaci in wild birds, cloacal swabs from 527 songbirds, 442 waterfowl, 84 feral pigeons, and 38 cormorants were examined by Chlamydiaceae-specific real-time polymerase chain reaction (PCR) and ArrayTube microarray assays for chlamydial species determination and genotyping of C. psittaci. Inconclusive cases were further characterized by conventional PCR methods targeting the chlamydial outer membrane protein A, 16S, 23S, and intergenic spacer genes followed by sequencing of the PCR product. Swabs of 19 water birds (tufted ducks and pochards), 12 pigeons, and one songbird were tested positive by the Chlamydiaceaespecific real-time PCR. While C. psittaci genotypes B (n= 5) and E (n=1) were identified in feral pigeons (n=9), the genotype could not be identified in the remaining three cases. Sequence data of Chlamydiaceae-positive cases (n= 23; 19 waterfowl, three pigeons, one songbird) indicated the presence of nonclassified chlamydial agents (n=20) and C. psittaci (n=3) by 16S rRNA PCR and sequencing. In conclusion, C. psittaci was not detected in waterfowl and songbirds, but C. psittaci proved prevalent in urban feral pigeons, where it poses a significant risk for humans.
Detection of Chlamydophila psittaci from pigeons by polymerase chain reaction in Ahvaz
Iranian Journal of Microbiology, 2015
Background and Objective: Chlamydophila psittaci is a lethal bacterium that causes endemic avian chlamydiosis, and respiratory psittacosis. Laboratory diagnosis of Chlamydophila psittaci is difficult by culture. This study was designed to investigate the presence of Chlamydophila psittaci in collected pharyngeal swabs from asyptomatic pigeons by PCR. Materials and Methods: Pharyngeal samples from pigeons with no symptoms of disease (n=280) were collected during hot and cold seasons in different parts of Ahvaz. DNA was extracted from specimens and subjected to PCR targeting pmp genes and 16s-23s rRNA intergenic spacer of Cp. psittaci and Chlamydiales specific primers. Results: Of 280 samples 2 (0.7%) harbor were positive for chlamydiales (16s-23s intergenic spacer) and Cp. psittaci specific genes (pmp gene). Conclusions: In this research the pigeons were asymptomatic carriers for Cp. psittaci in their respiratory discharges. These results suggest that Cp. psittaci infection of human can occur in very close and continuous contact with pigeons.
Journal of Clinical Microbiology, 1994
Five commercially available immunoassays were evaluated for the detection of Chlamydia psittaci in cloacal and conjunctival swabs from industrially raised turkeys: IMAGEN (DAKO Diagnostics, Ely, Cambridgeshire, United Kingdom), Chlamydia CEL-VET IF (Cellabs, Brookvale, Australia), IDEIA (DAKO Diagnostics), CELISA (Cellabs), and CLEARVIEW (Unipath, Bedford, United Kingdom). Results were compared with isolation in Buffalo Green Monkey cells as a reference method. For the conjunctival samples, the sensitivities of the IMAGEN test, the Chlamydia CEL-VET IF test, the IDEIA, the CELISA, and the CLEARVIEW test were found to be 100, 66, 0, 0, and 0%, respectively, as compared to the reference test. Also for the conjunctival samples, the specificities of the IMAGEN test, the Chlamydia CEL-VET IF test, and the IDEIA were found to be 100, 11, and 92.8%, respectively. For the cloacal specimens, the sensitivities of the IMAGEN test, the Chlamydia CEL-VET IF test, the IDEIA, the CELISA, and the C...
Revista Argentina De Microbiologia, 2019
In order to determine the presence and genetic diversity of Chlamydia spp. in the northeastern area of Buenos Aires province, Argentina, conjunctival, oropharyngeal, cloacal swab and tissues were collected from a total of 90 psittacine pet birds of different age and clinical manifestations. Through molecular methods, Chlamydiaceae was detected in 30% (27/90) of the samples, out of which 70.3% (19/27) were positive for Chlamydia psittaci and 14.9% (4/27) for Chlamydia abortus. Nine C. psittaci positive samples were genotyped by ompA gene sequences, 8 clustered within genotype A and 1 within genotype B. A significant association was observed between the presence of Chlamydia spp. and the manifestation of clinical signs compatible with chlamydiosis, as well as with the age of the birds (younger than one year old). This report contributes to the improvement of our understanding of chlamydial agents in our country.