Validation of a fluorescent microsphere multiplex serology assay for differential diagnosis of exposure to Zika virus and other closely related arboviruses (original) (raw)

The Journal of Immunology

Abstract

Arboviruses cause a serious public health problem in tropical and subtropical countries and are emerging as a threat in western hemisphere also. Rapid and accurate detection of these infections can help in improving patient care and disease control. The cross-reactivity of immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies to ZIKV with dengue virus (DENV), West Nile virus (WNV), yellow fever virus (YFV) and some other related flaviviruses poses a significant challenge for an accurate detection of ZIKV infection in serological assay such as IgM capture ELISA. To overcome these challenges we developed a multiplex serological assay that will simultaneously detect antibody response to ZIKV, other related flaviviruses and Chikungunya virus (CHIKV). Various recombinant arboviral antigens were coupled to optically encoded microspheres from Luminex Corporation. This fluorescent microsphere immunoassay was used to differentiate between recent, past, and/or co-infections that may oc...

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