Lignans, Amides, and Saponins from Haplophyllum tuberculatum and Their Antiprotozoal Activity (original) (raw)

Screening of six medicinal plant species for antileishmanial activity

Acta Pharmaceutica

This study is aimed to investigate the in vitro anti-leishmanial activity of ethanolic, aqueous or dichloromethane extracts of leaves, flowers, fruits or roots, of six medicinal plant species, namely, Nectandra megapotamica, Brunfelsia uniflora, Myrcianthes pungens, Anona muricata, Hymenaea stigonocarpa and Piper corcovandesis. After isolation and analysis of chemical components by ultra-high performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS), the extracts were also tested for toxicity in J774.A1 macrophages and human erythrocytes. Phenolic acids, flavonoids, acetogenins, alkaloids and lignans were identified in these extracts. Grow inhibition of promastigotes forms of Leishmania amazonensis and Leishmania braziliensis and the cytotoxicity in J774.A1 macrophages were estimated by the XTT method. The most promising results for L. amazonensis and L. braziliensis were shown by the ethanolic extract of the fruits of Hymenaea stigonocarpa and dichl...

Preliminary Phytochemical Screening of Five Plants as Possible Antileishmaniasis Control Agent

Journal of Complementary and Alternative Medical Research, 2020

Leishmaniasis is a major public health problem globally and manifests in three clinical forms including visceral cutaneous and mucocutaneous. Visceral leishmaniasis is fatal if left untreated for a period of 2 years, while cutaneous leishmaniasis cause crusted papules or ulcers on exposed skin. Plant families containing active compounds against other protozoan diseases may be suitable against leishmania parasites. This study report the compounds extracted from five plants (Olea europaea, Kigelia Africana, Terminalia mollis, Croton macrostachyus and Bridella micrantha extracts). The plants were collected from Baringo County in Kenya and authenticated at the National Museums of Kenya (Department of Botany). The plant samples were dried, pulverized into fine powders and extracted using methanol at the Center for Traditional Medicine and Drugs Research, KEMRI. The plant extracts contained varying amounts of phytochemical compounds such as tannins, phenols, flavonoids, steroids, alkaloid...

Antileishmanial Activity of Selected Turkish Medicinal Plants

Purpose: To determine the in vitro and in vivo anti-leishmanial activities of extracts obtained from Centaurea calolepis, Phlomis lycia, Eryngium thorifolium, Origanum sipyleum and Galium incanum ssp. centrale. Methods: To estimate the cytotoxicity of plant extracts, WST-1 assay was used. Parasite inhibition in the presence of plant extracts (25 - 500 μg/ml) in comparision with control group and reference group (glucantime, 25 μg/ml) at 12 - 72 h were determined in vitro on L. tropica promastigotes. The in vivo leishmanicidal activity of the extracts was evaluated against L. tropica-infected mice with glucantime as reference drug. Results: The chloroform extract of Galium incanum ssp. centrale showed the highest cytotoxicity with IC50 value of 0.0316 ± 0.005 μg/ml. In vitro parasite inhibition by the plant extracts ranged between 16.7 ± 0.01 % and 100 ± 0.00 % at 25 μg/ml concentration. The methanol extract of Eryngium thorifolium possessed the highest activity on promastigotes of L. tropica with 100 % inhibition at 25 μg/ml. The water and chloroform extracts of C. calolepis and water and methanol extracts of E. thorifolium at a dose of 100 mg/kg reduced parasitaemia in L. tropica infected mice. Conclusion: Parasite viability results suggest that the methanol extract of Eryngium thorifolium, regarded as non-cytotoxic, is a promising candidate drug for treating L. tropica infection.