In Vitro Germplasm Preservation of Lily Species Utilizing Callus Cultures at Low Temperature (original) (raw)

In vitro preservation of 21 lily species including some endangered ones was tried. Bulb scales of lily plants grown in vitro were placed on gellan gum-solidified MS medium containing 1 or 10 mg/l picloram and 30 g/l sucrose, and cultured at 25 under 16 h photoperiod at 44µmol m-2 S-1. After 2 months of culture, callus formation was initiated on these media at the basal part of the scale segments in all species. For proliferation, these calli were transferred to fresh medium of the same composition and cultured at the same environmental conditions. The calli initiated on medium supplemented with 1 mg/l picloram showed more vigorous growth than those with 10 mg/l picloram. All of the calli thus maintained for more than one year showed original ploidy level as assessed by flow cytometry. For regeneration, calli of sixteen out of 21 species maintained for more than one year were transferred to 1/2 MS medium containing 5 g/l sucrose and no phytohormone. The calli of all species except for Lilium davidii showed plant regeneration ability. Then calli of L. formosanum var. pricei, L. lancifolium var. flaviflorum, L. leucanthum var. centifolium, L. monadelphum and L. wallichianum proliferated on MS medium containing 1 mg/l picloram were transferred to the same fresh medium and cultured at several temperature regimes. As the results, these calli grew slowly at low temperature, which could consequently be used for long preservation of lily germplasm with reduced investments of labour for regular transfer.

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