Serum Apolipoprotein A-I and Large High-Density Lipoprotein Particles Are Positively Correlated with FEV1 in Atopic Asthma (original) (raw)
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Journal of lipid research, 2017
Blood eosinophil counts and serum periostin levels are biomarkers of type 2 inflammation. Although serum levels of HDL (high-density lipoprotein) and apolipoprotein A-I have been associated with less severe airflow obstruction in asthma, it is not known whether serum lipids or lipoprotein particles are correlated with type 2 inflammation in asthmatics. Here, we assessed whether serum lipids and lipoproteins correlated with blood eosinophil counts or serum periostin levels in 165 atopic asthmatics, and 163 non-asthmatic subjects with and without atopy. Serum lipids and lipoproteins were quantified using standard laboratory assays and nuclear magnetic resonance (NMR) spectroscopy. Absolute blood eosinophils were quantified by complete blood counts. Periostin levels were measured using the Elecsys Periostin assay. In atopic asthmatics, blood eosinophils negatively correlated with serum HDL-cholesterol and total HDL particles measured by NMR spectroscopy (HDLNMR). Serum periostin levels...
Serum lipoproteins are not associated with the severity of asthma
Pulmonary pharmacology & therapeutics, 2018
Asthma is a chronic inflammatory disorder of the bronchi with a complicated and largely unknown pathogenesis. In this context, an emerging role is attributed to the apolipoproteins which serve as structural components of plasma lipoproteins. Low density lipoproteins (LDL) may be involved in the inflammatory pathways of the asthmatic airways; in particular, small dense LDL (sdLDL) particles were associated with increased oxidative susceptibility compared to medium and large sized LDL. In our previous study, we found a positive correlation between forced expiratory volume 1 s (FEV) % predicted and larger LDL particles (LDL-1), and an inverse correlation between FEV% predicted and sdLDL (LDL-3) in mild, untreated asthmatics. Although LDL appear to be important modulators of inflammation, data on their clinical implications are still lacking. The aim of the study is to investigate whether LDL subclasses correlate with the severity of asthma, assuming that the atherogenic and most pro-in...
Serum low density lipoprotein subclasses in asthma
Respiratory Medicine, 2013
Background: The levels of serum low-density lipoproteins (LDL) have been implicated in the inflammatory cascade in a murine model of asthma. Recent findings suggest that LDL may modulate the inflammatory state of the asthmatic airways in humans. Objective: We explored whether LDL subclasses are associated with the occurrence and severity of asthma. Methods: 24 asthmatics (M/F: 11/13) and 24 healthy individuals, with normal BMI and absence of metabolic syndrome, matched for age and gender. Serum concentrations of LDL subclasses were distributed as seven bands (LDL-1 and-2 defined as large, least pro-inflammatory LDL, and LDL-3 to À7 defined as small, most pro-inflammatory LDL), using the LipoPrint ª System (Quantimetrix Corporation, Redondo Beach, CA, USA). Results: LDL-1 was similar in the two groups (56 AE 16% vs. 53 AE 11, p Z NS), while LDL-2 was significantly lower in asthmatics as compared to controls (35 AE 8% vs. 43 AE 10%, p Z 0.0074). LDL-3 levels were twofold higher in the asthmatics, but the difference did not reach the statistical significance (8 AE 7.3% vs. 4 AE 3%, p Z NS). Smaller subclasses LDL-4 to LDL-7 were undetectable in controls. In asthmatics, LDL-1 was positively associated with VC% predicted (r Z þ0.572, p Z 0.0035) and FEV 1 % predicted (r Z þ0.492, p Z 0.0146). LDL-3 was inversely correlated with both VC% predicted (r Z À0.535, p Z 0.0071) and FEV 1 % predicted (r Z À0.465, p Z 0.0222). Conclusions: The findings of this pilot study suggest a role of LDL in asthma, and advocate for larger studies to confirm the association between asthma and dyslipidemia.
Lipid mediator profiles differ between lung compartments in asthmatic and healthy humans
The European respiratory journal, 2014
Oxylipins are oxidised fatty acids that can exert lipid mediator functions in inflammation, and several oxylipins derived from arachidonic acid are linked to asthma. This study quantified oxylipin profiles in different regions of the lung to obtain a broad-scale characterisation of the allergic asthmatic inflammation in relation to healthy individuals.
Small airways function and molecular markers in exhaled air in mild asthma
Thorax, 2005
Background: Several studies suggest that the periphery of the lung is the major site of inflammation in asthma. Fractional exhaled nitric oxide (FeNO) and 8-isoprostane have been proposed as biomarkers of inflammation and oxidative stress. We therefore hypothesised that small airway dysfunction in asthma is of inflammatory origin that can be detected by molecular markers in exhaled air. To test this hypothesis, we examined the relationship of FeNO and 8-isoprostane in exhaled air with small airways function as assessed by the single breath nitrogen test. Methods: Sixteen patients (14 women) with mild atopic asthma (forced expiratory volume in 1 second .80% predicted) of mean (SD) age 23.0 (5.5) years participated in a cross sectional study. FeNO was recorded by chemiluminescence and 8-isoprostane was measured by ELISA in concentrated exhaled breath condensate. The slope of phase III (dN 2) and the closing volume (CV) were assessed from the single breath washout curve. Results: The median FeNO level was 30.4 ppb (range 10.1-82.8), the median 8-isoprostane concentration in exhaled breath condensate was 2.2 pg/ml (range 1.6-2.7), and the mean (SD) dN 2 value was 1.1 (0.4)% N 2 /l. FeNO was positively associated with dN 2 (r s = 0.54, p = 0.032) while 8-isoprostane was inversely correlated with FEV 1 % predicted (r s = 20.58; p = 0.017) and CV as a percentage of vital capacity (r s = 0.58; p = 0.019). Conclusions: FeNO and 8-isoprostane in exhaled air are associated with small airways function in mild asthma. This suggests that these markers reflect small airway inflammation and favours a role for them as disease markers that is complementary to spirometry in the monitoring of patients with asthma. Abbreviations: CV, closing volume; EBC, exhaled breath condensate; FeNO, fractional exhaled nitric oxide; FEV 1 , forced expiratory volume in 1 second; FVC, forced vital capacity; PC 20 MCh, provocative concentration of methacholine causing a 20% fall in FEV 1-À Deceased December 2004.
Fatty acids in serum cholesteryl esters in relation to asthma and lung function in children
Clinical <html_ent glyph="@amp;" ascii="&"/> Experimental Allergy, 2006
Background Dietary fatty acid intake has been proposed to contribute to asthma development with n-6 polyunsaturated fatty acids (PUFA) having a detrimental and n-3 PUFA a protective effect. Objective The aim of our analysis was to explore the relationship between fatty acid composition of serum cholesteryl esters as marker of dietary intake and prevalence of asthma, impaired lung function and bronchial hyper-responsiveness in children. Methods The study population consisted of 242 girls and 284 boys aged 8-11 years, living in Munich, Germany. Data were collected by parental questionnaire, lung function measurement and skin prick test according to the International Study of Asthma and Allergies in Childhood phase II protocol. Confounder-adjusted odds ratios (OR) with 95% confidence intervals (CI) were calculated for the association between quartiles of fatty acid concentration and health outcomes with the first quartile as reference. Results n-3 PUFA: levels of eicosapentaenoic acid were not related to asthma and impaired lung function. Linolenic acid levels were positively associated with current asthma (OR for fourth quartile 3.35, 95% CI 1.29-8.66). Forced expiratory volume in 1 s (FEV 1) values decreased with increasing levels of linolenic acid (p for trend = 0.057). n-6 PUFA: there was a strong positive association between arachidonic acid levels and current asthma (OR 4th quartile 4.54, 1.77-11.62) and a negative association with FEV 1 (P = 0.036). In contrast, linoleic acid was negatively related to current asthma (OR 4th quartile 0.34, 0.14-0.87) and FEV 1 values increased with increasing levels of linoleic acid (P = 0.022). The ratio of measured n-6 to n-3 PUFA as well as levels of palmitic and oleic acid were not consistently related to asthma or lung function. Conclusion Our data do not support the hypothesis of a protective role of n-3 PUFA. Elevated arachidonic acid levels in children with asthma may be because of a disturbed balance in the metabolism of n-6 PUFA or may be secondary to inflammation in these patients.
Low serum high-density lipoprotein cholesterol in childhood is associated with adolescent asthma
Paediatric Respiratory Reviews, 2011
Inflammation, endothelial activation and oxidative stress have been established as key events in the initiation and progression of atherosclerosis. High-density lipoprotein cholesterol (HDL-c) is protective against atherosclerosis and coronary heart disease, but its association with inflammation, endothelial activation and oxidative stress is not well established.
TOF-SIMS analysis of exhaled particles from patients with asthma and healthy controls
European Respiratory Journal, 2012
Particles in exhaled air (PEx) may reflect the composition of respiratory tract lining fluid (RTLF); thus, there is a need to assess their potential as sources of biomarkers for respiratory diseases. In the present study, we compared PEx from patients with asthma and controls using time-of-flight-secondary ion mass spectrometry (TOF-SIMS) and multivariate analysis.