No Difference Between High-Fructose and High-Glucose Diets on Liver Triacylglycerol or Biochemistry in Healthy Overweight Men (original) (raw)

Abstract

BACKGROUND & AIMS: Diets high in fructose have been proposed to contribute to nonalcoholic fatty liver disease. We compared the effects of high-fructose and matched glucose intake on hepatic triacylglycerol (TAG) concentration and other liver parameters. DESIGN: In a double-blind study, we randomly assigned 32 healthy but centrally overweight men to groups that received either a high-fructose or high-glucose diet (25% energy). These diets were provided during an initial isocaloric period of 2 weeks, followed by a 6-week washout period, and then again during a hypercaloric 2-week period. The primary outcome measure was hepatic level of TAG, with additional assessments of TAG levels in serum and soleus muscle, hepatic levels of adenosine triphosphate, and systemic and hepatic insulin resistance. RESULTS: During the isocaloric period of the study, both groups had stable body weights and concentrations of TAG in liver, serum, and soleus muscle. The high-fructose diet produced an increase of 22 AE 52 mmol/L in the serum level of uric acid, whereas the high-glucose diet led to a reduction of 23 AE 25 mmol/L (P < .01). The high-fructose diet also produced an increase of 0.8 AE 0.9 in the homeostasis model assessment of insulin resistance, whereas the high-glucose diet produced an increase of only 0.1 AE 0.7 (P ¼ .03). During the hypercaloric period, participants in the high-fructose and high-glucose groups had similar increases in weight (1.0 AE 1.4 vs 0.6 AE 1.0 kg; P ¼ .29) and absolute concentration of TAG in liver (1.70% AE 2.6% vs 2.05% AE 2.9%; P ¼ .73) and serum (0.36 AE 0.75 vs 0.33 AE 0.38 mmol/L; P ¼ .91), and similar results in biochemical assays of liver function. Body weight changes were associated with changes in liver biochemistry and concentration of TAGs. CONCLUSIONS: In the isocaloric period, overweight men who were on a high-fructose or a high-glucose diet did not develop any significant changes in hepatic concentration of TAGs or serum levels of liver enzymes. However, in the hypercaloric period, both high-fructose and high-glucose diets produced significant increases in these parameters without any significant difference between the 2 groups. This indicates an energymediated, rather than a specific macronutrientmediated, effect. Clinical trials.gov no: NCT01050140.

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References (47)

  1. Thoma C, Day CP, Trenell MI. Lifestyle interventions for the treatment of non-alcoholic fatty liver disease in adults: a systematic review. J Hepatol 2012;56:255-266.
  2. Lim JS, Mietus-Snyder M, Valente A, et al. The role of fructose in the pathogenesis of NAFLD and the metabolic syndrome. Nat Rev Gas- troenterol Hepatol 2010;7:251-264.
  3. Cox CL, Stanhope KL, Schwarz JM, et al. Consumption of fructose- but not glucose-sweetened beverages for 10 weeks increases circulating concentrations of uric acid, retinol binding protein-4, and gamma-glutamyl transferase activity in overweight/obese humans. Nutr Metab (Lond) 2012;9:68.
  4. Stanhope KL, Schwarz JM, Keim NL, et al. Consuming fructose- sweetened, not glucose-sweetened, beverages increases visceral adiposity and lipids and decreases insulin sensitivity in overweight/ obese humans. J Clin Invest 2009;119:1322-1334.
  5. Brundin T, Wahren J. Whole body and splanchnic oxygen consump- tion and blood flow after oral ingestion of fructose or glucose. Am J Physiol 1993;264:E504-E513.
  6. Livesey G. More on mice and men: fructose could put brakes on a vicious cycle leading to obesity in humans. J Am Diet Assoc 2011; 111:986-990; author reply 990-993.
  7. Sievenpiper JL, de Souza RJ, Mirrahimi A, et al. Effect of fructose on body weight in controlled feeding trials: a systematic review and meta-analysis. Ann Intern Med 2012;156:291-304.
  8. Ouyang X, Cirillo P, Sautin Y, et al. Fructose consumption as a risk factor for non-alcoholic fatty liver disease. J Hepatol 2008;48:993-999.
  9. Marriott BP, Cole N, Lee E. National estimates of dietary fructose intake increased from 1977 to 2004 in the United States. J Nutr 2009;139:1228S-1235S.
  10. White JS. Straight talk about high-fructose corn syrup: what it is and what it ain't. Am J Clin Nutr 2008;88:1716S-1721S.
  11. Le KA, Faeh D, Stettler R, et al. A 4-wk high-fructose diet alters lipid metabolism without affecting insulin sensitivity or ectopic lipids in healthy humans. Am J Clin Nutr 2006;84:1374-1379.
  12. Bantle JP, Raatz SK, Thomas W, et al. Effects of dietary fructose on plasma lipids in healthy subjects. Am J Clin Nutr 2000; 72:1128-1134.
  13. Saturated fat recommended level. Scientific Advisory Committee on Nutrition 2008.
  14. Henry CJ. Basal metabolic rate studies in humans: measurement and development of new equations. Public Health Nutr 2005; 8:1133-1152.
  15. Hagstromer M, Oja P, Sjostrom M. The International Physical Activity Questionnaire (IPAQ): a study of concurrent and construct validity. Public Health Nutr 2006;9:755-762.
  16. Szczepaniak LS, Babcock EE, Schick F, et al. Measurement of intracellular triglyceride stores by H spectroscopy: validation in vivo. Am J Physiol 1999;276:E977-E989.
  17. Vanhamme L, van den Boogaart A, Van Huffel S. Improved method for accurate and efficient quantification of MRS data with use of prior knowledge. J Magn Reson 1997;129:35-43.
  18. DeFronzo RA, Tobin JD, Andres R. Glucose clamp technique: a method for quantifying insulin secretion and resistance. Am J Physiol 1979;237:E214-E223.
  19. Powrie JK, Smith GD, Hennessy TR, et al. Incomplete suppression of hepatic glucose production in non-insulin dependent diabetes mel- litus measured with [6,6-2H2]glucose enriched glucose infusion during hyperinsulinaemic euglycaemic clamps. Eur J Clin Invest 1992;22:244-253.
  20. Kury D, Keller U. Trimethylsilyl-O-methyloxime derivatives for the measurement of [6,6-2H2]-D-glucose-enriched plasma samples by gas chromatography-mass spectrometry. J Chromatogr 1991; 572:302-306.
  21. Le KA, Ith M, Kreis R, et al. Fructose overconsumption causes dys- lipidemia and ectopic lipid deposition in healthy subjects with and without a family history of type 2 diabetes. Am J Clin Nutr 2009; 89:1760-1765.
  22. Nelson M, Bates B, Church S, et al. Low income diet and nutrition survey. London, UK: The Stationery Office; 2009.
  23. McDevitt RM, Poppitt SD, Murgatroyd PR, et al. Macronutrient disposal during controlled overfeeding with glucose, fructose, sucrose, or fat in lean and obese women. Am J Clin Nutr 2000; 72:369-377.
  24. Stanhope KL, Griffen SC, Bair BR, et al. Twenty-four-hour endocrine and metabolic profiles following consumption of high-fructose corn syrup-, sucrose-, fructose-, and glucose-sweetened beverages with meals. Am J Clin Nutr 2008;87:1194-1203.
  25. Tappy L, Le KA, Tran C, et al. Fructose and metabolic diseases: new findings, new questions. Nutrition 2010;26:1044-1049.
  26. Parra MD, Martinez de Morentin BE, Martinez JA. Postprandial insulin response and mitochondrial oxidation in obese men nutri- tionally treated to lose weight. Eur J Clin Nutr 2005;59:334-340.
  27. Silbernagel G, Machann J, Unmuth S, et al. Effects of 4-week very- high-fructose/glucose diets on insulin sensitivity, visceral fat and intrahepatic lipids: an exploratory trial. Br J Nutr 2011;106:79-86.
  28. Perez-Pozo SE, Schold J, Nakagawa T, et al. Excessive fructose intake induces the features of metabolic syndrome in healthy adult men: role of uric acid in the hypertensive response. Int J Obes (Lond) 2010;34:454-461.
  29. Rao SS, Attaluri A, Anderson L, et al. Ability of the normal human small intestine to absorb fructose: evaluation by breath testing. Clin Gastroenterol Hepatol 2007;5:959-963.
  30. Rumessen JJ, Gudmand-Hoyer E. Absorption capacity of fructose in healthy adults. Comparison with sucrose and its constituent mono- saccharides. Gut 1986;27:1161-1168.
  31. Ali A, Becker E, Chaudhury M, et al. Health Survey for England etc 2006. Edited by Craig R & Mindell J. Published by the National Health Service Information Centre, Leeds, UK.
  32. Statistics on alcohol: England 2010. Responsible Statistician East- wood P, National Health Service Health Information Centre, Leeds, UK.
  33. Tran C, Jacot-Descombes D, Lecoultre V, et al. Sex differences in lipid and glucose kinetics after ingestion of an acute oral fructose load. Br J Nutr 2010;104:1139-1147.
  34. Malik VS, Schulze MB, Hu FB. Intake of sugar-sweetened beverages and weight gain: a systematic review. Am J Clin Nutr 2006;84:274-288.
  35. Sievenpiper JL, Carleton AJ, Chatha S, et al. Heterogeneous effects of fructose on blood lipids in individuals with type 2 diabetes: sys- tematic review and meta-analysis of experimental trials in humans. Diabetes Care 2009;32:1930-1937.
  36. Ngo Sock ET, Le KA, Ith M, et al. Effects of a short-term overfeeding with fructose or glucose in healthy young males. Br J Nutr 2010; 103:939-943.
  37. Chong MF, Fielding BA, Frayn KN. Mechanisms for the acute effect of fructose on postprandial lipemia. Am J Clin Nutr 2007;85:1511-1520.
  38. Tilg H, Hotamisligil GS. Nonalcoholic fatty liver disease: cytokine- adipokine interplay and regulation of insulin resistance. Gastroen- terology 2006;131:934-945.
  39. Schaffler A, Scholmerich J, Buchler C. Mechanisms of disease: adi- pocytokines and visceral adipose tissue-emerging role in nonalco- holic fatty liver disease. Nat Clin Pract Gastroenterol Hepatol 2005; 2:273-280.
  40. Nielsen S, Guo Z, Johnson CM, et al. Splanchnic lipolysis in human obesity. J Clin Invest 2004;113:1582-1588.
  41. Musso G, Cassader M, De F, et al. Nonalcoholic steatohepa- titis versus steatosis: adipose tissue insulin resistance and dysfunc- tional response to fat ingestion predict liver injury and altered glucose and lipoprotein metabolism. Hepatology 2012;56:933-942.
  42. Sobrecases H, Le KA, Bortolotti M, et al. Effects of short-term overfeeding with fructose, fat and fructose plus fat on plasma and hepatic lipids in healthy men. Diabetes Metab 2010;36:244-246.
  43. Aeberli I, Hochuli M, Gerber PA, et al. Moderate amounts of fructose consumption impair insulin sensitivity in healthy young men: a ran- domized controlled trial. Diabetes Care 2013;36:150-156.
  44. Abdelmalek MF, Lazo M, Horska A, et al. Higher dietary fructose is associated with impaired hepatic adenosine triphosphate homeo- stasis in obese individuals with type 2 diabetes. Hepatology 2012; 56:952-960.
  45. Israel KD, Michaelis OE, Reiser S, et al. Serum uric acid, inorganic phosphorus, and glutamic-oxalacetic transaminase and blood pressure in carbohydrate-sensitive adults consuming three different levels of sucrose. Ann Nutr Metab 1983;27:425-435.
  46. Wang DD, Sievenpiper JL, de Souza RJ, et al. The effects of fructose intake on serum uric acid vary among controlled dietary trials. J Nutr 2012;142:916-923.
  47. Ferrannini E, Natali A, Bell P, et al. Insulin resistance and hyperse- cretion in obesity. European Group for the Study of Insulin Resistance (EGIR). J Clin Invest 1997;100:1166-1173.