Non-lethal DNA sampling of wing tips discriminates subspecies ofApis melliferaoccurring in Greece (original) (raw)
Related papers
Apidologie, 2005
The genetic structure and phylogenetic relationships among six honey bee populations were studied using RFLP analysis on three PCR-amplified mtDNA gene segments (16s rDNA, CO I, and ND 5). The populations were sampled from various areas of Greece and Cyprus and correspond to Apis mellifera adami, A. m. macedonica, A. m. cecropia, and A. m. cypria races, based on origin . Seven, eight and seven restriction enzymes were found to have at least one recognition site at the 16s rDNA, CO I, and ND 5 segments, respectively. Seven different haplotypes were detected and diagnostic patterns enabled us to discriminate A. m. macedonica from the rest of the populations (races). The estimated net nucleotide sequence divergence among the populations examined was found to range from 0.00 to 1.18 with the highest value observed to be between A. m. macedonica and non-A. m. macedonica populations. The trees obtained (by UPGMA and Dollo parsimony methods) revealed that the most distant population was that of A. m. macedonica.
Bulgarian Journal of Agricultural Science, 2009
KEKECOGLU, M., M. BOUGA, M. I. SOYSAL and P. HARIZANIS, 2009. Genetic divergence and phylogenetic relationships of honey bee populations from Turkey using PCR-RFLP’s analysis of two mtDNA segments. Bulg. J. Agric. Sci., 15: 589-597 The genetic structure and phylogenetic relationship among honey bee populations of Turkey were studied using RFLP analysis on two PCR-amplified mtDNA gene segments (COI, 16s rDNA). The honey bees were sampled from 54 mainland localities of Turkey and 2 Aegean islands. Two different mitotype were detected with SspI digestion of COI gene. One mitotype was seen in only central Anatolia. The results of this research were compared with analogous studies on honey bee populations from Greece and it was found that the non-existence of 16s rDNA /DraI digestion is diagnostic only for Turkish honey bee populations. This result is very useful for the control of conservation of local honey bees, as the movement of colonies across the border line of these neighboring c...
Analysis of mitochondrial DNA in honey bees (Apis mellifera) from Serbia
Acta veterinaria, 2007
In this study mitochondrial DNA of the indigenous honey bee populations from Serbia was analysed. Three ecotypes were comprised in the investigation: Banat (B), Syenichko-Peshterski (S) and Timok (T). The results show that all investigated samples of honey bees from Serbia belong to one mtDNA haplotype of the C phylogenetic lineage, previously designated as C2D haplotype, though with three additional polymorphic sites. For the novel mtDNA haplotype, the designation C2E is proposed. The novel C2E haplotype differs from C2D in two A-T transversions and in one insertion. Moreover, within the T honey bee ecotype, two additional polymorphic sites were observed; a deletion of a T nucleotide and a T-C transition. Our results suggest that the Carniolan honey bee populations from Serbia may represent an indigenous gene pool within A. m. carnica. The mtDNA haplotype (C2D), previously found in A. m. macedonica, was found in all samples of Serbian A. m. carnica. This suggests that the C2D haplotype may be found within both subspecies. This study represents the first effort to characterize the indigenous honey bee populations in Serbia using molecular techniques with the objective of improving our understanding of the phylogeography of Apis mellifera.
2019
Hybridization between honey bee subspecies is common and hybrid bees can completely or partial replace the endemic bee subspecies as happened in some countries including Egypt. This study aimed to develop a new method to identify the maternal source of hybrid bees and to compare between sequences. The method (Similarity percentages of nucleotides) depends on using specific numerical system to nucleotides. The method was tested to identify the maternal source of hybrid bees from two honey bee subspecies Apis mellifera scutellata and Apis mellifera capensis based on similarity percentages in mtDNA sequences. The mtDNA sequence of the hybrid bees was more similar to A. m. capensis than A. m. scutellata, suggesting that the maternal source of the hybrid bees belonged to A. m. capensis. Results of the shared gene cluster analysis and similarity percentages based on 13 genes confirmed the similarity trend between the two subspecies and their hybrid. Additionally, specific primers to separate the two subspecies than their hybrid were designed based on the results of similarity percentages and tested using SnapGene. The trend of data analysis used in this study showed high ability to detect the similarities between the two subspecies and their hybrid. Also, this method can be used to identify the maternal source of hybrid bees including those exposed to a long period of hybridization (e.g. hybrid bees of Egypt), to specify the identical parts of the sequence, and to design specific primers to separate subspecies than their hybrids. The method presented in this study is likely to be very important tool to specify the maternal source of breeding stocks during genetic improvement programs of honey bees.
Apidologie, 2009
To identify the evolutionary lineage of honey bee colonies in Turkey, the mtDNA of 244 colonies from 20 locations was analyzed. Several polymorphic restriction sites showed that they belonged to the Mediterranean C lineage. DraI digestion of the CoxI-CoxII intergenic region produced four fragment patterns, one first seen in this study. From 37 colonies from 16 different locations in Turkey and two colonies from Iran, the intergenic region was sequenced. Previously, from among all honey bee populations of the C lineage, eight haplotypes had been described from this mtDNA region, three of which were found here. In addition, eight new haplotypes were found. A deletion in one of these haplotypes accounts for the novel DraI RFLP pattern. Most of the novel haplotypes were in a subgroup of lineage C, yet none of these had been found in previous studies of Turkish honey bees. The geographical distribution of some haplotypes suggests that they may be characteristic of subspecies native to Turkey.