Deciphering the traits associated with PAH degradation by a novel Serratia marcesencs L-11 strain (original) (raw)
Related papers
International Journal of Environment, 2015
Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria were isolated from prolong contaminated Amalakhadi sediment and crude oil polluted soil Telva, near Ankleshwar Gujarat India. Organisms were treated with two-model PAHs compound Anthracene (ANT), and Pyrene (PYR) as the sole source of carbon and energy. Identification of the isolates was carried out based on their morphological and partial 16S rRNA gene sequences, which revealed that the isolates belong to two main bacterial groups: gram-negative pseudomonas indoxyladons and gram-positive, spore-forming group, Bacillus benzoevorans. GC-MS based degradation study demonstrated that P. indoxyladons efficiently degrade 98% of ANT and PYR by 93.2 % when treated with 250 mg L-1. However, B. benzoevorans could tolerate to 200 mg L-1of PYR. Thus, the findings of the study provide novel bacterial sp. having different capacity to degrade model PAHs compounds and further could be utilized for the standardization of bioremediation protoco...
Isolation and genetic identification of PAH degrading bacteria from a microbial consortium
Biodegradation, 2009
Polycyclic aromatic hydrocarbons (PAH; naphthalene, anthracene and phenanthrene) degrading microbial consortium C2PL05 was obtained from a sandy soil chronically exposed to petroleum products, collected from a petrochemical complex in Puertollano (Ciudad Real, Spain). The consortium C2PL05 was highly efficient degrading completely naphthalene, phenanthrene and anthracene in around 18 days of cultivation. The toxicity (Microtox TM method) generated by the PAH and by the intermediate metabolites was reduced to levels close to nontoxic in almost 40 days of cultivation. The identified bacteria from the contaminated soil belonged to c-proteobacteria and could be include in Enterobacter and Pseudomonas genus. DGGE analysis revealed uncultured Stenotrophomonas ribotypes as a possible PAH degrader in the microbial consortium. The present work shows the potential use of these microorganisms and the total consortium for the bioremediation of PAH polluted areas since the biodegradation of these chemicals takes place along with a significant decrease in toxicity.
Bacteria-mediated PAH degradation in soil and sediment
Applied Microbiology and Biotechnology, 2011
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the natural environment and easily accumulate in soil and sediment due to their low solubility and high hydrophobicity, rendering them less available for biological degradation. However, microbial degradation is a promising mechanism which is responsible for the ecological recovery of PAH-contaminated soil and sediment for removing these recalcitrant compounds compared with chemical degradation of PAHs. The goal of this review is to provide an outline of the current knowledge of biodegradation of PAHs in related aspects. Over 102 publications related to PAH biodegradation in soil and sediment are compiled, discussed, and analyzed. This review aims to discuss PAH degradation under various redox potential conditions, the factors affecting the biodegradation rates, degrading bacteria, the relevant genes in molecular monitoring methods, and some recent-year bioremediation field studies. The comprehensive understanding of the bioremediation kinetics and molecular means will be helpful for optimizing and monitoring the process, and overcoming its limitations in practical projects.
Applied Microbiology: open access, 2015
Polycyclic aromatic hydrocarbons (PAHs) are common environmental pollutants and biodegradation using microorganisms is the preferred and major route of PAH removal from contaminated environments. This study investigated the bacterial degradation of petrol and diesel in liquid media that were isolated from oil contaminated soils by enrichment technique. The isolates could use petrol and diesel as their sole carbon and energy source in Bushnell Hass Mineral Salts (BHMS) medium at 2% (v/v) concentration. A total of eight isolates were selected and characterized by using a variety of phenotypic and morphologic properties. Two isolates each showed highest growth in petrol and diesel containing media during screening were selected and characterized using 16S RNA sequencing. Molecular identification of the isolates assigned them to Achromobacter sp. and Pseudomonas aeruginosa. The selected isolates degraded petrol and diesel up to 31.9% and 34.4% respectively. This study indicates that the contaminated soil samples contain a diverse population of PAH-degrading bacteria and the use of Achromobacter sp. and Pseduomonas aeruginosa has the potential for bioremediation of PAH contaminated sites.
World Journal of Microbiology and Biotechnology, 2020
Although the use of degrading-bacteria is one of the most efficient methods for the bioremediation of polluted sites, detection, selection and proliferation of the most efficient and competing bacteria is still a challenge. The objective of this multi-stage research was to investigate the effects of the selected bacterial strains on the degradation of anthracene, florentine, naphthalene, and oil, determined by biochemical tests. In the first stage, using the following tests: (a) biosurfactant production (emulsification, oil spreading, number of drops, drop collapse, and surface tension), (b) biofilm production, (c) activity of laccase enzyme, and (d) exopolysaccaride production, the three bacterial strains with the highest degrading potential including Bacillus pumilus, B. aerophilus, and Marinobacter hydrocarbonoclasticus were chosen. In the second stage using the following tests: (a) bacterial growth, (b) laccase enzyme activity, and (c) biosurfactant production (emulsification, oil spreading, and collapse of droplet) the degrading ability of the three selected bacterial strains plus Escherichia coli were compared. Different bacterial strains were able to degrade anthracene, florentine, naphthalene, and oil by the highest rate, three days after inoculation (DAI). However, M. hydrocarbonoclasticus showed the highest rate of florentine degradation. Although with increasing pollutant concentration the degrading potential of the bacterial strains significantly decreased, M. hydrocarbonoclasticus was determined as the most efficient bacterial strain.
Microbes using PAHs as Energy source: Relationship with diseases
Research Journal of Biotechnology, 2016
Bioremediation is known as a natural process which relies on bacteria and fungi to degrade, to breakdown and to remove various contaminants such as petroleum hydrocarbons from soil and water. These microorganisms possess the capability to use petroleum hydrocarbons as energy source and consequently render the contaminants harmless or less toxic. Various substances with known toxic properties are continuously being introduced into the environment due to human activities. These contaminants pose great risk to human health and cause serious damage to environment and biodiversity. Polycyclic aromatic hydrocarbons (PAHs) have been detected in high concentrations at sites that use or process petroleum products. PAHs are found to be carcinogens and very recalcitrant and persist in natural environment for several years. In this review our main focus is on biodegradation of PAHs (pollutant) by different bacterial and fungal groups and the different pathways that are used by these microbial groups to degrade PAHs. PAHs are not only an environmental hazard but are found to be a good source of energy to these microbes and hence their degradation not only relieves the environment of this environmental pollutant but provides the basic building blocks for microbes to survive. Bioremediation has tremendous potential to provide a cost-effective natural process to render toxic substances in environment harmless overtime.
Fakultas Perikanan dan Ilmu Kelautan, Universitas Jenderal Soedirman
The main purpose of the study was conducted to isolate PAHs-degraders strain from the Donan river mangroves ecosystem and to investigate the ability of isolated pure culture to degrade PAHs. The potential use of these marine bacteria as the environment clean-up agents was conducted by sublimating with the 6 different Polyaromatic hydrocarbons (PAHs) compounds as a contaminant model such as phenothiazine, fluorene, fluoranthene, dibenzothiophene, phenanthrene, and pyrene. The 16S rDNA amplification using primer 9F and 1510R has been applied and purification was made on the agarose (1 %). Sequenced results were obtained by comparing to NCBI Blast. Three rods shape Gram-positive endospore-forming bacteria that were isolated from a mangrove site which is chronically contaminated from petroleum hydrocarbons. On the basis of phenotypic and phylogenetic data, three strains should be placed in the genus Bacillus as three distinct species, for which the names Bacillus aquimaris, Bacillus megaterium, and Bacillus pumilis are proposed. The other three strains were Flexibactereae bacterium, Halobacilus trueperi, and Rhodobacteraceae bacterium.
Egyptian Journal of Pure and Applied Science, 2021
Received 24 August 2021 Accepted 03 November 2021 Soil contamination with petroleum derivatives, including polycyclic aromatic hydrocarbons (PAHs), is an increasing problem in various parts of the world. Dynamics of bacterial communities were examined in different petroleumcontaminated soils to assess patterns of microbial responses to PAH contamination. Bacterial community fingerprints were determined using denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene as a molecular marker. Sequence-based identification of dominant DGGE bands in petroleum hydrocarbon-contaminated soils revealed the presence of Pseudomonas sp., P. pseudoalcaligenes, P. nitroreducens, P. toyotomiensis, and uncultured Pseudomonas clones as the dominant bacterial groups. In a laboratory scale microcosm, prolonged incubation of contaminated soils resulted in marked deterioration of bacterial communities. DGGE fingerprints of PAH-amended microcosms indicated the presence of distinct bands corresponding...