Anti-Helicobacter pylori IgG in Asymptomatic Population (original) (raw)
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DETECTION OF ANTI-HELICOBACTER PYLORI IgG ANTIBODIES IN HEALTHY SUBJECTS
…, 2011
Introduction: Helicobacter pylori (H. pylori) are Gram-negative microaerophilic, spiral organisms. Factors such as family history of gastric disease, source of drinking water, number of siblings, sharing beds, and level of hygiene have been linked to acquisition of H. pylori infection. Most of the infected people do not have clinical symptoms. The study was planed to determine the level of anti-H. pylori IgG antibodies in the serum of healthy individuals. Material and Methods: The study included 80 healthy subjects and was conducted in the Department of Immunology, University of Health Sciences Lahore. The studied population was divided on the basis of (a) eating food from outside home daily, twice a week or once a week, (b) using filtered or tap water for drinking, and (c) having family history of gastric ulcer or without family history of gastric ulcer. Level of anti H. pylori IgG antibody was determined by ELISA technique. Results: Among 80 asymptomatic healthy individuals anti-H pylori IgG antibody was detected in 28 (35%) subjects who did not have these antibodies (p-value < 0.001). Mean level of anti-H. pylori IgG antibodies was 43 ± 39.3 U/ml, 30.7 ± 37.3 U/ml and 14.9 ± 19.7 U/ml in subjects eating food from outside their homes once a week, twice a week and daily respectively. Statistically significant difference was observed in the level of H. pylori antibodies with different eating habits (p = 0.015). However no statistically significant difference was observed in the level of anti-H. pylori antiodies between two genders, individuals using tap water and filtered water for drinking and with family history of gastric ulcer. Conclusion: H. pylori IgG antibodies were present in asymptomatic healthy individuals of both the genders.
Clinical and Vaccine Immunology, 2002
The clinical significance of high levels of antibody against Helicobacter pylori is still unclear. We sought to evaluate whether the serum antibody levels could predict the presence of macroscopic gastroduodenal disease, to identify factors that correlate with antibody levels in a multivariate context, and to determine the predictive value of antibody levels for diagnosing H. pylori infection. The grades of gastritis and density of H. pylori colonization were scored separately using the updated Sydney system for antral and body mucosa. An enzyme-linked immunosorbent assay (ELISA) for the quantitative detection in serum of IgG antibodies to H. pylori was performed. Of the 170 dyspeptic patients, 105 (62%) had H. pylori infection. There was no difference in antibody levels among endoscopic findings of normal mucosa, chronic gastritis, and duodenal ulcer. On multivariate linear regression analysis, the status of H. pylori infection, mononuclear cell infiltration of body mucosa, and age correlated with antibody levels. The negative predictive value for antibody levels of &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;30 U/ml is 94%, and the positive predictive value of antibody levels of &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;70 U/ml is 98%. We conclude that serum antibody levels do not predict the severity of gastroduodenal diseases or the density of H. pylori colonization in H. pylori-infected dyspeptic patients. Higher levels are associated with the presence of H. pylori infection, the chronic gastritis score of the corpus, and older age. Setting a gray zone is necessary for ELISA, since the accuracy in this zone does not allow a precise determination of H. pylori status.
Evaluation the Efficacy of ELISA IgG, IgM and IgA Tests for Diagnosis of Helicobacter pylori
Kurdistan Journal of Applied Research
It has been well recognized throughout the world that Helicobacter pylori is the main cause of gastric ulcer and stomach carcinoma. Laboratory diagnosis of H. pylori infection is made by invasive and non-invasive methods. Invasive methods require endoscopy which is uncomfortable an unacceptable by the most patients. Therefore, non-invasive methods particularly serological tests are easier and comfortable for patients. A total of 86 patients with ages ranging from 18-77 years old (43 males and 43 females) who were referred to the Duhok Hepatology & Gastroenterology center/Azadi Teaching Hospital for endoscopic examination from June to October, 2013 were enrolled in the study. From each patient 5 ml of blood was collected under a septic condition and sera were separated for serology. Data from each participant were recorded in a special questionnaire form after consent agreed upon on ethical and scientific committee of the Azadi hospital. The efficacy of three different ELISA tests ...
1998
Several commercially available serological kits have been used as an alternative to endoscopy for the diagnosis of Helicobacter pylori infection. We evaluated the performance of two such kits, Serion H. pylori immunotab kit (Serion, Wurzberg, West Germany) and Pyloragen H. pylori test kit (Hypcor Biomedical Inc., Irvine, CA). Gastric biopsy and serum samples were collected from 345 consecutive dyspeptic patients. The culture and or direct smear of the biopsy was positive for H. pylori in 228 patients (66%), whereas 117 patients (34%) were found to be H. pylori negative. We determined the serological response of the patients using the two kits, both of which are based on the principle of enzyme-linked immunosorbent assay. Comparing the serum immunoglobulin G (IgG) and IgA (in a limited number of cases) responses to H. pylori status, the sensitivity, the specificity, positive predictive value, and negative predictive value were calculated. The corresponding data for the different tests were 64%, 79%, 84%, and 56% for Serion IgG, 32%, 94%, 88%, and 52% for Serion IgA, and 88%, 17%, 62%, and 46% for Pyloragen IgG, respectively. We conclude that there is a poor correlation between the presence of H. pylori infection and the antibody response, which could be explained either because of low sensitivities and specificities of the commercial kits used for the measurement of antibodies to H. pylori in the serum or because of poor immunological response in our patients to H. pylori antigens.
Alimentary Pharmacology and Therapeutics, 2005
Background: The performance of commercial Helicobacter pylori diagnostic kits developed for particular geographic regions has often been found to be of poor diagnostic value when applied to other regions, possibly because of infections being caused by different H. pylori strains in different regions. Aim: To evaluate the performance of an IgG2 anti-H. pylori enzyme-linked immunoassay test (Helirad Alert) for detection of H. pylori infection in both Australian and Hong Kong (Chinese) subjects. Methods: Serum samples were tested for H. pylori specific IgG2 and IgG antibodies by enzyme-linked immunoassay kits using identical antigen preparation in 168 Australian and 160 Hong Kong (Chinese) subjects diagnosed with dyspepsia. Results: Using a cutoff value determined by analysis of H. pylori-negative Australian samples, the sensitivity, specificity and accuracy of the IgG2 assay were 77.8, 97.4 and 91.1%, respectively, for the Australian samples and 96.3, 83.8 and 90% for Hong Kong samples. For the IgG assay, sensitivity, specificity and accuracy were 87.0, 99.1 and 95.2% for Australian samples and 97.5, 75 and 86.3% for Hong Kong samples respectively. Receiver-operating characteristic analysis showed better discrimination of H. pylori status when the IgG2 assay was applied to Hong Kong samples, while the IgG assay was better in the Australian samples. Conclusion: These data demonstrate that the Helirad Alert enzyme-linked immunoassay could provide a reliable method for screening H. pylori infection in both western and Chinese populations.
Diagnostic Microbiology and Infectious Disease, 1998
Several commercially available serological kits have been used as an alternative to endoscopy for the diagnosis of Helicobacter pylori infection. We evaluated the performance of two such kits, Serion H. pylori immunotab kit (Serion, Wurzberg, West Germany) and Pyloragen H. pylori test kit (Hypcor Biomedical Inc., Irvine, CA). Gastric biopsy and serum samples were collected from 345 consecutive dyspeptic patients. The culture and or direct smear of the biopsy was positive for H. pylori in 228 patients (66%), whereas 117 patients (34%) were found to be H. pylori negative. We determined the serological response of the patients using the two kits, both of which are based on the principle of enzyme-linked immunosorbent assay. Comparing the serum immunoglobulin G (IgG) and IgA (in a limited number of cases) responses to H. pylori status, the sensitivity, the specificity, positive predictive value, and negative predictive value were calculated. The corresponding data for the different tests were 64%, 79%, 84%, and 56% for Serion IgG, 32%, 94%, 88%, and 52% for Serion IgA, and 88%, 17%, 62%, and 46% for Pyloragen IgG, respectively. We conclude that there is a poor correlation between the presence of H. pylori infection and the antibody response, which could be explained either because of low sensitivities and specificities of the commercial kits used for the measurement of antibodies to H. pylori in the serum or because of poor immunological response in our patients to H. pylori antigens.
Journal of Health Science, 2008
Helicobacter pylori (H. pylori) infection is common worldwide. Although the seropositivity of H. pylori rates has been unclear in the Turkish population. In this study, anti-H. pylori IgG seroprevalence and anti-cytotoxin-associated gene A (CagA) IgG positivity were evaluated. The sera of 880 people without gastrointestinal symptoms (384 males, 496 females) were tested for anti-H. pylori IgG and anti-CagA IgG antibodies by enzyme linked immunoassay method. Anti-H. pylori IgG antibodies were positive in 263 sera (41%) and their rates increased with age. The seroprevalence of anti-H. pylori IgG was higher in females (43.8%) than in males (38%). Of the anti-H. pylori IgG positive sera, 194 (53%) were also positive for anti-CagA IgG. The anti-CagA IgG positivity did not significantly differ with age. However, the lowest rate (46.6%) was determined among individuals 20-29 years of age and the highest rate (62.5%) among individuals over 60 years age. Anti-CagA IgG positivity rates were higher in males (87.5%) than in females (37.5%).
International Journal Of Medical Science And Clinical Invention, 2021
is the most common chronic bacterial infection (acquired early childhood) in humans affecting 50% of the world population and much attention has not been paid to this. This study was carried out between February and October 2019 to test for the presence of Helicobacter Pylori antibody among asymptomatic individuals attending Federal Medical Center, Yenagoa, Bayelsa State. A total of 200 {114(57%) males, 86(43%) females} blood samples were collected at ramdom into Ethylene diamine tetraacetic acid (EDTA) bottles and immediately transported to the laboratory for analysis using Helicobacter pylori Serology rapid blood test kit. Age was stratified to allow for comparison because the entire outcome was age dependent. Chi square analysis was conducted for the categorical variable. Findings showed that out of 200 samples examined, 88(44%) forty (40 (45%) males and 48(55%) females were positive to Helicobacter pylori infection while 112(56%) were negative. Females of age range 24-33 had the highest prevalence of 24 (27%) while male of age group 14 to 23 had 21(24%); females of 34 to 44 was 16(18%); 54-63 had 4(05%) and the least was 44 to 53 years with prevalence of 3(03%). There was a significant difference across the age group and socio-demographic characteristic at p-value = 0.0001 < 0.05 and p-value =0.002068 < 0.05, p-value = 0.000916 <0.05 respectively. Observations showed the higher prevalence in females (53%) than their males (47%) counterparts; likewise the infected individuals host this organism ignorantly and busy treating out of line. Study with more than one diagnostic technique is recommended to determine the presence of Helicobacter pylori, as rapid blood test is limited due to the presence of antibody in the serum for long after eradication. In conclusion, routine medical examination on Helicobacter pylori is encouraged among individuals in respective of age and status.
Clinical and Vaccine Immunology, 2003
The identification of Helicobacter pylori-strain specific factors that correlate with clinical outcome has remained elusive. We investigated possible relationships between a group of H. pylori antigens and clinical outcome and compared an immunoblot assay kit (HelicoBlot, version 2.1 [HB 2.1]; Genelabs Diagnostics) with an established serological test, the high-molecular-weight cell-associated protein test (HM-CAP). We used sera from 156 Thai patients with different disease presentations, including 43 patients with gastric cancer, 64 patients with gastric ulcer, and 49 patients with nonulcer dyspepsia (NUD). HB 2.1 was compared to HM-CAP as a diagnostic test for H. pylori infection. The seroprevalence of H. pylori was significantly higher among gastric cancer patients than among patients with NUD (93 and 67%, respectively; P < 0.01). Among the H. pylori-seropositive patients, the presence of the antibody to the 37,000-molecular-weight antigen (37K antigen) was inversely related to the presence of gastric cancer (e.g., for gastric cancer patients compared with NUD patients, odds ratio [OR] ؍ 0.28 and 95% confidence interval [CI] ؍ 0.1 to 0.8). The presence of antibody to the 35K antigen was higher in gastric ulcer patients than in NUD patients (OR ؍ 11.5; 95% CI ؍ 2.4 to 54.3). The disease associations of antibodies to the 35K and 37K antigens are consistent with the possibility that these antigens are either indirect markers for H. pylori-related diseases or have specific active or protective roles in H. pylori-related diseases.