Kemampuan Kapang Dark Septate Endophyte dari Akar Tanaman Aren dalam Menghambat Ganoderma sp (original) (raw)
Related papers
Agrikultura
Ganodema boninense merupakan penyebab penyakit busuk pangkal batang pada tanaman kelapa sawit yang dapat menimbulkan kerugian secara ekonomi di Provinsi Riau. Salah satu teknik pengendalian yang dapat dilakukan adalah penggunaan jamur endofit sebagai agens hayati. Penelitian ini bertujuan untuk menguji kemampuan antagonisme jamur endofit dari tanaman aren terhadap G. boninense penyebab penyakit busuk pangkal batang kelapa sawit. Penelitian ini dilaksanakan di Laboratorium Penyakit Tanaman, Fakultas Pertanian, Universitas Riau pada bulan Juli hingga November 2020. Penelitian dilaksanakan dengan metode eksplorasi, eksperimen dan observasi. Isolasi dari daun, batang dan akar tanaman aren menemukan 25 isolat jamuer endofit. Di antara isolat jamur endofit terdapat tujuh isolat yang menghambat pertumbuhan G. boninense sebesar 50,17% - 67,05% yaitu 67,05% (E2), 66,11% (E16), 61,43% (E18), 52,22% (E15), 51,23% (E3), 50,72% (E17) dan 50,17% (E25). Pada pengamatan mikroskopis , jamur endofit ...
2017
Penggunaan mikroorganisme antagonis merupakan salah satu alternatif pencegahan penyakit busuk pangkal batang yang disebabkan oleh Ganodermaspp. pada perkebunan kelapa sawit. Penelitian ini bertujuan untuk mendapatkan cendawan endofit asal tanaman kelapa sawit yang berpotensi sebagai agens biokontroluntuk mengendalikan Ganodermapadapembibitan kelapa sawit. Penelitian dilaksanakan di Rumah Kaca Fakultas Pertanian USU pada Juni 2015 sampai Januari 2016 menggunakanRancanganAcakKelompoknon faktorial dengan enam perlakuan yaitu Aspergillus sp1 + Ganoderma, Aspergillus sp2 + Ganoderma, Rhopalomyces sp + Ganoderma, Chrysosporiumsp + Ganoderma, Gongronella sp1 + Ganoderma, Gongronella sp2 + Ganoderma dan tiga ulangan.Hasil penelitian menunjukkan bahwa pengaruh pemberian cendawan endofit asal tanaman kelapa sawitdapat meningkatkan pertumbuhan kelapa sawit pada tanah terinfeksi Ganoderma,dengan daun terluas pada perlakuan Rc (Rhopalomyces sp. + Ganoderma) yaitu 987,95 cm2dan perlakuan Ra (Aspe...
Karakteristik Cendawan Dark Septate Endophyte (Dse) Pada Akar Tanaman Jagung Dan Padi
EKSAKTA: Berkala Ilmiah Bidang MIPA
Plant roots host a variety of fungi. The association between fungi and plant roots categorized to mycorrhizal and non-mycorrhizal. One important group of non-mycorrhizal symbioses is the dark septate endophytes (DSE) that colonize plant roots and form characteristic intracellular structures-melanized hyphae and microsclerotia. Compared to better known mycorrhizal symbioses or the vertically transmitted systemic foliar endophytes, the DSE have received very little attention. So, the research was conduct to analize the characteristics of DSE in roots system of maize and rice plant. The roots sample was taken from maize and rice fields around Kuranji District, Padang. Occurance of DSE was observe by tryphan blue staining (0.05%) using digital microscope. DSE was found in 65% of the total of maize root segments observed and 30% for roots of rice plant. DSE occurs in corn roots system as brown hyphae inter-and intracellularly and dark-brown microsclerotia intracellularly. The smaller microsclerotia found in rice roots system and absorbed tryphan blue, then appeared as blue color. The melanized hyphae found in rice roots system was different with those found in maize root.
Jurnal Penelitian Karet
Cendawan Dark Septate Endophyte (DSE) adalah sekelompok cendawan endofit yang memiliki hifa melanin gelap, membentuk koloni berwarna gelap pada media agar dan mampu mengkolonisasi akar tanaman tanpa menyebabkan gejala penyakit. Belum ada laporan yang berkaitan dengan peran cendawan DSE sebagai agens pengendali penyakit akar putih, khususnya di Indonesia. Penelitian ini bertujuan untuk mendapatkan isolat DSE dari Indonesia dengan cara menseleksi isolat cendawan DSE yang berpotensi sebagai agensia hayati penyakit akar putih pada tanaman karet. Tahapan awal dari penelitian adalah isolasi cendawan DSE dari akar dan tanah di sekitar perakaran karet, uji patogensitas, uji dual culture dan uji metabolit senyawa volatil. Dalam penelitian ini, diperoleh lima isolat cendawan DSE APDS 3.2, TBMDS 2.4b, TMDS 2.1, TMDS 3.2 dan SBTBMDS 1 yang mampu menghambat pertumbuhan Rigidoporus microporus penyebab penyakit jamur akar putih pada tanaman karet. Penelitian ini membuktikan bahwa isolat cendawan D...
Jurnal Hama dan Penyakit Tumbuhan Tropika
Assay on ability of endophytic bacteria isolated from peanut to inhibit Sclerotium sp. growth in peanut seedlings. A study on assay of ability of endophytic bacteria to inhibit Sclerotium sp. in peanut seedling has been done. The bacteria were isolated from peanut healthy plants, while Sclerotium sp. was isolated from infected peanaut plant. Antagonistic assay was conducted by dual culture method. In vivo assay of inhibiting Sclerotium sp. was conducted by dipping peanut seed in bacterial solution, and planting the seed in soil:compost (3:1) growing media. Six endophytic bacterial isolates showed to inhibit the growth of Sclerotium sp. in vitro. LN1 seemed to inhibit more of Sclerotium sp., while LN5 showed to inhibit less. Two potential isolates LN1 of gram-negative and LN2 of gram-positive using for further study showed to decrease more of dumping off. It also seemed that the isolates increased the seedling height, number of leaves, and dry weight.
Penelitian ini bertujuan untuk mendapatkan jamur endofit dengan kemanpuan antagonis terhadap G. boninense dari tanaman kelapa sawit (.8'. Guinemsis). Ganoderma boninense adalah jamur penyebab penyakit busuk pangkal batang (basal stem rot) pada kelapa sawit. Jamur endofit diisolasi dari akar tanaman kelapa sawit yang sehat dan telah disterilisasi pemrukaannya. Isolat yang diperoleh dikarakterisasi dan diuji kemampuan antagonisnya terhadap jarln G.boninense. Lokasi pengambilan sampel yaitu perkebunan sawit di daerah Medaq Taqjung Morawa, Pematang Siantar, Rantau Prapat dan Asahan. Hasil isolasi diperoleh 30 isolat jamur endofit yang terdiri dari 6 genus yaitu Trichoderma, Penicillium, Aspergillus, Mucor, Cochliobolus, dan Rhizamucor. Jamur endofit paling banyak ditemukan pada perkebunan sawit rakyat Rantau Prapat Utara.
Jurnal Mikologi Indonesia
Keberadaan jamur endofit dalam jaringan tanaman dapat menguntungkan, merugikan atau tidak berpengaruh terhadap tanaman inangnya. Penelitian ini bertujuan untuk mengkaji pengaruh jamur endofit yang diisolasi dari akar dan daun kacang tanah terhadap pertumbuhan tanaman kacang tanah dan kemampuannya dalam menghambat patogen inangnya yaitu Sclerotium rolfsii dan Cercospora sp. secara in vitro. Sampel tanaman diperoleh dari daerah Panyingkiran (Kabupaten Majalengka), Jatinangor (Kabupaten Sumedang) dan Arjasari (Kabupaten Bandung). Isolat jamur endofit akar yang diperoleh diuji patogenesitasnya terhadap kecambah dan bibit tanaman kacang tanah. Isolat jamur endofit dari daun diuji patogenesitasnya dengan menginokulasikannya pada daun kacang tanah. Isolat jamur endofit akar yang bersifat non patogenik diuji kemampuan antagonistiknya terhadap S. rolfsii, sedangkan isolat yang berasal dari daun diuji terhadap Cercospora sp.secara in vitro. Hasil pengujian menunjukkan bahwa dari 13 isolat jam...
Jurnal Penelitian Kehutanan Sumatrana
Sugar palm (Arenga pinnata Merr.) is an important plant that has many benefits and economic value. Indonesian people in general have known the usefulness of this plant because of its many benefits, almost all parts of the palm plant can be utilized. Currently, its use still relies heavily on wild plants that grow in the forest. One of the obstacles that causes the cultivation of aren is less desirable is the problem of germination of the sugar palm seeds that have a long dormancy period that can reach up to 1 year. This study was aimed to find ways of scarification that are easy and inexpensive in breaking the sugar palm dormancy to be easily applied by the public in rural areas. The study was conducted using a completely randomized design with three replications and 4 treatments in the form of scratching the middle of the seed + soaking (LT), scratching the edge + soaking (LP), burning + soaking (B), soaking in vixal solution (V), and control (K ). The results showed the best way o...
Jurnal Tanaman Industri dan Penyegar, 2021
Colletotrichum leaf fall disease caused by Colletotrichum gloeosporioides is an important disease in rubber plants. The C. gloeosporioides Infection can reduce production by 7%-45%. Controlling the pathogen using endophytic fungi is very promising because it can suppress inoculum and pathogen colonization, induce plant resistance, and trigger plant growth. The study aimed to evaluate the endophytic fungus isolate from rubber to control C. gloeosporioides as a pathogen that caused the rubber leaf fall disease. This research was carried out in the laboratory and greenhouse of the Indonesian Industrial and Beverage Crop Research Institute (IIBCRI), Sukabumi, from March to November 2018. The isolates used were endophytic fungi isolates from rubber plants, which were tested for their inhibition against C. gloeosporioides in vitro on rubber leaves and seedlings, and their mechanism. The variable observed were the inhibition rate, incubation periods, number of spots, disease severity, and ...
PENDIPA Journal of Science Education
Peanut (Arachis hypogaea L.) is one of the important crops in Indonesia due to its nutritional content richness. Consumption of peanuts will continue to increase along with the population growth in Indonesia. However, production of peanut did not meet the national consumption needed. The low productivity was caused by several number of technical factors such as tillage, fertilization, harvesting, pest and disease control, and the use of low-quality seeds. The low quality of peanut seeds may be caused by seed-borne fungi such as Aspergillus flavus which lead to seed abortion and the change of nutritional content. The use of fungi metabolites can be used as an effective and efficient alternative control. This study was aimed to obtain endophytic fungi that have the potential as biological control agents for A. flavus. In this research, we evaluate this purpose by A. flavus and endophytic fungi isolation from peanut tissue, pathogenicity test, inhibition test against A. flavus, extraction and metabolite testing of endophytic fungi. Based on the results of the pathogenicity test, there are 33 endophytic fungi which are non-pathogenic. We select the best three isolates based on its mechanisms in inhibiting A.