Evaluation of induced spawning on oocyte characteristics and serum biochemistry of African catfish (Clarias gariepinus) (original) (raw)

Seasonal reproductive biology and artificial propagation of female African catfish (Clarias gariepinus) after hormonal stimulation

Journal of Applied Plant Protection; Suez Canal University, 2014

The use of exogenous hormones to induce final oocyte maturation, ovulation, and spawning has become commonplace in the reproductive protocols for many species. In the present study, African catfish (Clarias gariepinus) were monthly collected from January to December 2011 for studying their monthly reproductive biology. Also, hormonal stimulation was done using ovaprim, human chorionic gonadotropin (hCG), and salmon gonadotropin releasing hormone analog (GnRHa). However, fish (337.2-718.0 g) were divided into seven groups (each group contained four females and four males) and injected into the dorsal musculature by 0.4, 0.5 ml kg-1 Ovaprim, 1000, 3000 IU kg-1 hCG, and 0.75, 1 ml kg-1 GnRHa. The control group was injected by 0.9% saline. At the dosage tested, gonado-somatic index (GSI), absolute fecundity, ovulation index, egg diameter, fertilization rate were significantly affected in all stimulated groups. It was found that the hormonal treatments are a useful method for commercial African catfish breeders to ensure the continuity of access larvae. However, the best reproductive performance was obtained at 0.5 ml kg-1 ovaprim, 3000 IU kg-1 hCG, and 0.75 ml kg-1 GnRHa.

Gonadosomatic index and some hematological parameters in African catfish Clarias gariepinus (Burchell, 1822) as affected by feed type and temperature level

Brazilian Archives of Biology and Technology, 2017

The purpose of the current study was to examine the effects of different feeds/nutrients and temperature on the gonadal development of Clarias gariepinus. The gonadosomatic index (GSI) and blood parameters including red blood cell count (RBCs), white blood cell count (WBCs), hemoglobin (Hgb) level, hematocrit (HCT), platelets (PLT) and mean corpuscular volume (MCV) were investigated. Four types of fish feed of 36% protein content [D1(fish meal), D2 (soybean meal), D3 (peameal) and D4 (commercial tilapia feed)] and 3 different levels of temperature (T1(24℃), T2(28℃) and T3(32℃) were tried in this study for a duration of 4 months. The mean values were as follows: