Carbon Dioxide Induced Soybean Protein Precipitation: Protein Fractionation, Particle Aggregation, and Continuous Operation (original) (raw)
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Protein extraction and purification by lime treatment and ultrafiltration on soybean flakes and meals is an environmentally friendly process that promises a novel alternative to conventional chemical treatment methods. Protein was extracted from soybean flakes and meals by ionic-strength of lime as alkali treatment. After centrifugation, proteins were purified by ultrafiltration.Lime treatedflakes and meals showed significantly higher level of dissolved solid, protein, and carbohydrate extraction rate than conventional sodium hydroxide or water treatment. Soybean flakes represented a higher extraction rate of protein and carbohydrate than meals. This result may becauseby extensive cell distortion and disruption with cracking, cooking, and flatting which allow lime solutes to easily permeate the cellular matrix. Ultrafiltration substantiallypurified the protein with minor loss of yields, 94.42% and 96.79% for soybean flakes and meals, respectively. Therefore, lime treatment and ultrafiltration is a viable option for extraction and purifying proteins of soybean flakes and meals