Callus Induction, Proliferation, Enhanced Secondary Metabolites Production and Antioxidants Activity of Salvia moorcroftiana L. as Influenced by Combinations of Auxin, Cytokinin and Melatonin (original) (raw)
Related papers
2015
Method for mass multiplication of callus of Salvia santolinifolia by nodal explants was investigated by optimizing the concentration and combinations of different plant growth regulators in MS medium. Nodal explants could be stimulated to form callus on MS medium supplemented with 1-Naphthalene acetic acid (NAA) (0.1, 0.5 and 1.0 mg/l). Large scale multiplication of callus was achieved on MS medium containing NAA (0.3 mg/l) plus N Benzylaminopurine (BAP) (1.5 mg/l) and NAA (0.3 mg/l) plus N-(2-isopentyl)-adenine (2iP) (1.0 mg/l). The phytochemical investtigation of callus lead to the isolation of β-Sitosterol and Stigmasterol compounds.
Plant Cell, Tissue and Organ Culture (PCTOC)
The presented study analyses the influence of salicylic acid (SA) and the synthetic auxin 1-naphthaleneacetic acid (NAA) on total tanshinone level and on dihydrotanshinone (DHT), cryptotanshinone (CT), tanshinine I (TI) and tanshinone IIA (TIIA) level in Salvia miltiorrhiza callus cultures growing on solid Murashige and Skoog (MS) medium. The influence of SA and NAA was evaluated at 10-day intervals throughout a 80-day treatment period. SA was applied at 0.1, 0.2 and 0.4 mM, and NAA at 2.69, 13.43, 26.85 and 40.28 μM. DHT, CT, TI and TIIA concentrations were measured using HPLC. NAA did not increase the concentration of any tanshinone. SA increased content in a concentration- and time-dependent manner; however, the yields were relatively low, possibly due to the metabolic specificity of S. miltiorrhiza cultivars in Poland. Total tanshinone concentration reached 226.38 ± 37.33 μg g−1 DW after 50 days of 0.4 mM SA elicitation. After 50 days of SA elicitation, the following maximum tan...
2015
Expression of natural products in callus culture established from nodal part of Salvia santolinifolia was investigated and the effect of age of culture and plant growth regulators was determined. Callus initiation started after 5-6 days of inoculation on MS1 medium and further proliferated of calli were obtained on MS2 & MS3 media. Subcultures of calli were regularly performed after 27-28 days of inoculation for nine months for the synthesis of secondary metabolites. With the age of callus maximum secondary metabolites accumulated after eight month of culture on both media (MS2 & MS3). A comparison of plant growth regulators on the bases of dry weight of callus showed that maximum synthesis of natural product occurred in MS3 medium. The phytochemical analysis of methanolic extract of callus tissues of Salvia santolinifolia reported stigmasterol for the first time. The structure of the isolated compound was determined by using mass spectrometry and 1D-& 2D-NMR techniques.
A Review Hairy Roots and Secondary Metabolite Production in Salvia
The roots of Salvia miltiorrhiza Bge named Danshen in traditional Chinese medicine, is widely used for the treatment of cardiovascular disorders, heart disease, stroke. Salvia miltiorrhiza Bunge, a well-known medicinal plant, has more than 20 effective components. In this review, results suggest that the tanshinone accumulation induced by the two elicitors was mainly synthesized via the non-MVA pathway (DXS activity), but could depend on crosstalk between the MVA and non-MVA pathways. Further In conclusion, phytohornones including ABA, GA3 and Ethylene were effective to improve production of phenolic acids and increase activitives of PAL and TAT in S. miltiorrhiza hairy roots. Antagonistic actions between the three phytohormones played important roles in the biosynthesis of phenolic acids.
Biological Sciences - PJSIR
Salvia santolinifolia is a medicinal plant and an efficient in vitro conservation system is established. The influence of N6Benzylaminopurine (BAP), N6-(2-isopentyl)-adenine (2iP) and Kinetin at various concentrations were evaluated, single and in mixture with NAA (Naphthalene acetic acid) for the production of auxiliary shoots from nodal explants of S. santolinifolia. BAP at 3.0 mg/L in MS1 media produced maximum (11.66±3.38) number of shoots while elongated (5.37±1.45) shoots were produced in the MS2 medium in subcultures at 2.0 mg/L of 2iP. Least number of shoots were formed when auxin and cytokinin were used in combination. Length of culture, age was an important consideration for the initiation and development of roots. Rooting of shoot was attained with Indol-3-butyric acid (IBA) (3.0 mg/L) from shoots of 4th, 5th and 6th subculture while shoots taken from 1st, 2nd and 3rd subculture failed to form roots.