The estimation of different ELISA procedures for serodiagnosis of human trichinellosis (original) (raw)
Related papers
Parasite (Paris, France), 2004
The use of serological tests to detect Trichinella infection in domestic and wild animals and in humans has not been standardised yet. This review provides an uniform set of recommendations for the development and use of serological tests to detect circulating antibodies in serum samples. The recommendations are based on the best scientific published information and on the unpublished data from laboratories with a great expertise in this field and represent the official position of the International Commission on Trichinellosis regarding acceptable methods and the evaluation of the sensitivity and specificity. These recommendations are subject to change as new scientific information becomes available.
Development and Evaluation of a Western Blot Kit for Diagnosis of Human Trichinellosis
Clinical and Vaccine Immunology, 2003
We evaluated industrially prepared Western blot strips designed to avoid the cross-reactions observed with indirect immunofluorescence and enzyme-linked immunosorbent assays used for the serodiagnosis of trichinellosis. The antigen preparations were crude extracts of Trichinella spiralis. The Western blot profile characteristic of trichinellosis was characterized by comparing 60 sera from patients infected by Trichinella to 11 sera from healthy subjects, 51 sera from patients with other proven parasitic diseases (cysticercosis, schistosomiasis, strongyloidosis, fascioliasis, toxocariasis, liver amebiasis, anisakiasis, filariasis, toxoplasmosis, hydatidosis, or malaria), and 23 sera from patients with autoantibodies. Specific 43-to 44-kDa and 64-kDa bands were obtained with all of the sera from 51 patients with acute trichinellosis, in 4 out of 9 patients at the early stages of the disease, and in only 1 control patient, who had suspected anisakiasis and in whom trichinellosis could not be ruled out by muscle biopsy.
Evaluation of Trichinella spiralis larva group 1 antigens for serodiagnosis of human trichinellosis
Journal of Clinical Microbiology, 2004
To identify Trichinella antigens suitable for high-specificity and high-sensitivity serodiagnosis of human trichinellosis, we evaluated assays using four antigens: (i) crude first-stage larval extract (CLE), (ii) O- deglycosylated CLE, (iii) tyvelose-bearing antigens (Trichinella spiralis larva group 1 (TSL-1) antigens) puri- fied by US4 affinity chromatography and coupled directly to enzyme-linked immunosorbent assay (ELISA) plates (pTSL-1 antigens), and (iv) TSL-1 antigens
Diagnosis of human trichinellosis : pitfalls in the use of a unique immunoserological technique
Parasite, 2001
Serum samples belonging to three outbreaks in Argentina (47 patients) taken at different times post-ingestion were analysed employing IIF and ELISA simultaneously. Results show that: a) the number of patients diagnosed by a unique technique, especially by ELISA (31 patients), was lower than the one obtained by the simultaneous use of both assays (38 patients); b) four patients out of the seven diagnosed by a unique technique were negative by the other assay over the period of time evaluated. Therefore, It can be concluded that the use of a sole immunoserological technique can not only lead to the delay in the detection but also to the misdiagnosis of this parasitic Infection.
Serological tools for detection of Trichinella infection in animals and humans
One health (Amsterdam, Netherlands), 2016
Trichinellosis is a serious foodborne zoonotic disease. It is an important threat to public health in both developing and developed countries. Human infections are strongly associated with consuming undercooked meat containing infective Trichinella larvae. The development of serological tools has enabled seroepidemiological studies and contributed to our knowledge on the importance of this parasite. Serological tests can also help the diagnosis of parasite infections in humans and the surveillance of animals. Generally speaking, serological techniques include detection methods for specific antibodies and for circulating parasite antigens in the serum or tissue fluids. Here, we present a comprehensive review of various methods used in the detection of antibodies against Trichinella and circulating parasite antigens in animals and humans.
The use of a synthetic antigen for the serological diagnosis of human trichinellosis
Parasite (Paris, France), 2001
Hosts infected with Trichinella produce antibodies specific for an epitope common to the TSL-1 family antigens. This epitope contained uncommon terminal 3, 6-dideoxy-D-arabinohexose (so called tyvelose) residues. The disaccharide moiety was synthesized and an immunodiagnostic assay was developed, which was specific and sensitive in swine trichinellosis. We aimed to verify the specificity and sensitivity of this immunodiagnostic test in human trichinellosis. 15 sera from normal subjects, 12 from patients with other parasitic diseases and 50 from trichinellosis patients were tested. Indirect enzyme linked immunosorbent assay (ELISA) for specific IgG and an amplified ELISA for specific IgE were performed using beta-tyvelose-GalNAc-bovine serum albumin (BSA) disaccharide conjugate or T. spiralis muscle larvae excretory/secretory (E/S) products, as antigens. Neither control sera nor other parasitic infection sera resulted positive both for IgG and IgE when synthetic or E/S antigens were ...
BJSTR, 2021
Trichinellosis is worldwide distributed zoonosis, which affects humans and other living organisms including mammals, birds, and reptiles. The distribution is facilitated by the modifications of ecosystems, and the repercussions on nature, such as increase of greenhouse gases and the increase of temperature. Having economic, social, and health repercussions. The present work aims to present the research carried out at the Autonomous University of Zacatecas from the year 1986 to 2021, on Trichinellosis parasitosis caused by parasite Trichinella spiralis, working on the biology of the parasite, the life cycle, experimental models, diagnosis, and treatments with drugs and immunogens. Methodology: Reproduction of the life cycle of Trichinella spiralis, in various experimental models: murine, dog, cat, rabbit, pig. Standardization of direct plate compression techniques, artificial digestion and hematoxylin-eosin and indirect double micro-immunodiffusion, DOT-ELISA, Immunofluorescence, Wester-blot, and intradermal reaction. Evaluation of drugs albendazole, quinfamide, products such as Lactobacilli, and immunomodulators such as VITS, immunogens the total extract of Trichinella spiralis, the 45 KDa band, and different immunization schemes and routes. Detection of the parasite in domestic rat, dog, pig and human. Elaboration of a diagnostic KIT. Results: In this project Long Evans rats and Balb-C mice were used as experimental murine models to maintain the parasite strain viable and maintain its pathogenicity characteristics (1986-2021). In all the reproduced models the direct and indirect techniques are useful and Wester-Blot shows a characteristic 42-45-48-KDa triplet. In experimental murine and pig models, the use of albendazole is effective against Trichinellosis with treatments of 14 days, destroying the nurse cell, likewise the use of Lactobacilli and VITS is effective and reduces the parasite load.On the other hand, treatments with immunogens, in the experimental models, the total soluble antigen, the 45 kDa band, was statistically significant in the different schemes and routes of administration. It was detected in domestic rats, pigs, dogs, and humans. Finally, a proposal of a diagnostic kit for the field, with the DOT-ELISA technique is in development. Conclusion: During the last decades it has been settled a well-established experimental murine model, for the study of the biology of Trichinella spiralis. Our research has shown that the correct use of albendazole as a treatment is effective.
Veterinary Parasitology, 2005
The performance characteristics of an ELISA test for trichinellosis in pigs applied to muscle juice was assessed using 314 samples collected from pigs located in endemic areas of Croatia. Peptic digestion was used as the reference method. The diagnostic accuracy of the two compared dilutions (1:10 and 1:100) was considered to be high because the area under the curve (AUC) index was 0.922 and 0.920 for each dilution, respectively. In this study the two graph-receiver operating characteristic (TG-ROC) analysis was used as a tool for selecting cut-off points. Sensitivity, specificity, likelihood ratios, efficiency and Youden's index were used as indices of test accuracy. The cut-off values that minimize overall misclassification cost under an assumption of 3% prevalence were calculated. Our results indicate that the ELISA applied to muscle juice is a highly accurate test and can be adapted to process a large number of samples. #
Veterinarski glasnik, 2019
Introduction. Trichinellosis is one of the most important foodborne diseases in Serbia. Most patients with suspected trichinellosis in Belgrade are referred to the Clinical Center of Serbia for diagnosis and treatment, as are unclear and complicated cases from all across Serbia. Materials and Methods. A retrospective study of trichinellosis serology was carried out from 2009-2018 and included all outpatients and hospitalised patients from the Clinic for Infectious and Tropical Disease, Clinical Center of Serbia, who were serologically tested for Trichinella by the Parasitological Laboratory (n=1,565). Trichinella-specific IgG antibodies were detected in sera by a commercial ELISA test. We analysed the seroprevalence of Trichinella-specific IgG antibodies, antibody detection kinetics and cross-reactivity with other nematodes. Results and Conclusions. The number of patients who reported for serological testing varied greatly per year and month. Most patients were tested in December and March, DAKIĆ Zorica et al.: Serological screening of trichinellosis by ELISA 145 which coincides with the months with the most confirmed cases of trichinellosis. A total of 17.4% patients who were tested for trichinellosis had other parasitic infections. Altogether, 223 (14.2%) of tested patients were finally diagnosed with trichinellosis. We detected anti-Trichinella IgG in 68.8% (223) of patients with suspected trichinellosis on admission, which increased to 86.5%, 91.5% and 92.4% after later second, third and fourth testing, respectively. Final diagnoses of toxocariasis, strongyloidiasis, filariasis, and dirofilariasis were made for 2.4%, 0.3%, 0.3% and 0.1% of patients, respectively. Concurrent seropositivity for Trichinella and Toxocara was observed in 18.9% (7/37) of patients with clinical presentation of trichinellosis and who were also tested for toxocariasis. In 3/5 patients with imported filariasis, we found cross-reactivity with Trichinella. Potential cross-reactivity of this ELISA test with antibodies to the autochthonous nematode Toxocara canis demands the introduction of Western blot technology. Trichinellosis must be diagnosed by the combination of clinical, laboratory and epidemiological criteria.