Simultaneous determination of lysophospholipids by high-performance liquid chromatography with fluorescence detection (original) (raw)
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Journal of Lipid Research, 1989
A novel, facile, and sensitive method for the quantitative and complete structure-proof analysis of platelet-activating factor (PAF) and other glycerophospholipids is described. l-O-Alkyl/ acyl-2-acyl-3-glycerophospholipids were treated with heptahorobutyric anhydride in a one-step reaction to yield l-O-alkyVacyl-2acyl-3-heptduorobutyroyl-sn-glycerols as gas-liquid chromatography (GLC)-compatible derivatives. Furthermore, the components treatment of glycerophospholipids generates two isomers of Abbreviations: PAF, platelet-activating factor; PMN, neutrophilic polymorphonuclear leukocyte; AGEPC, acetyl glyceryl ether phosphocholine; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphmhoIine; HFB, heplluorobutyroyl; PFB, pentafiuorobenzoyl; TBDMS, t-butyldmethylsilyl; TMS, trimethylsilyl; FAB, fast atom bombardment; GLC-MS, gas-liquid chromatography-mass spectrometry; EI, electron impact; CI, chemical ionization; EC, electron capture. 'To whom correspondence should be addressed.
Journal of chromatography. B, Biomedical applications, 1994
An improved high-performance liquid chromatographic method for the separation and determination of radioactively labelled cellular phospholipids is described. The method is based on separation of phospholipids on a 250 x 4 mm I.D. LiChrospher DIOL 100 (5 microns) column, fitted with a 50 x 4 mm I.D. LiChrospher Si 60 (5 microns) precolumn and a gradient of 5% H3PO4 and acetonitrile. It allows the determination of small amounts of labelled phosphatidylcholine and sphingomyelin due to the sharp elution profile in spite of long retention times.
Journal of Chromatography A, 1988
A method for the determination of plasmalogen, alkylacyl and diacyl glycerophospholipids based on mild alkaline deacylation and acid hydrolysis of plasmalogens on plates with subsequent micro-thin-layer chromatography on silica gel is presented. It is effective in quantifying alkyl and alkenyl analogues of phosphatidylethanolamine and phosphatidylcholine at concentrations up to 0.1% (of the total of the forms in individual classes of phospholipids).
Electrophoresis, 2018
This article is protected by copyright. All rights reserved. 2 (CM), , endoplasmic reticulum (ER), ethanol (EtOH), ether-insoluble PLS (EIP), free fatty acids (FFA), hexane/isopropanol (HIP), high-abundance proteins (HAPs), limit of detection (LOD), low-abundance proteins (LAPs), lyso-phosphatidylethanolamine (lyPE), lysophospholipids (LyPLS), methanol (MetOH), methyl-tert-buthyl ether (MTBE), origin of replication (oriC), phosphatidic acid (PA), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylserine (PS), phospholipids (PLS), pressurized liquid extraction (PLE), solid phase extraction (SPE), sphingomyelin (SM), supercritical fluids (SCF), thin-layer chromatography (TLC), triacylglicerol (TG), very-low density lipoproteins (VLDL).
A new enzymatic method for determination of serum choline-containing phospholipids
Clinica Chimica Acta, 1977
A new enzymatic method is presented for the determination of serum choline-containing phospholipids with a combined enzymatic method using phospholipase D (from Streptomyces species), choline oxidase (from Arthrobacter species) and peroxidase. The method is reproducible, and the results correlate well with those obtained by the conventional digestion method (Hoeflmayr, J. and Fried, R. (1966) Med. Ernaehr. 7, 9–10). The method affords better specificity, requires a smaller quantity of the sample and shorter time than those previously reported, and has excellent precision.
The molecular species composition of individual diacyl phospholipids in human platelets
Biochimica et biophysica acta, 1982
The molecular species composition of the individual diacyl phospholipids was determined in human platelets. The 1-acyl (16:0, 18:0, etc.) homologues of the various 2-acyl (16:0, 18:1, 18:2, 20:4, etc) species of the phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol were assessed by the use of thin-layer and gas-liquid chromatography in combination with specific lipases for establishing the positional distribution of the fatty acyl chains and generating the diacylglycerol derivatives. A marked heterogeneity was found in the complement of individual molecular species associated with the different platelet phospholipids. The 1-16:0 2-18:1 species predominated in the phosphatidylcholine (21% of total) but contributed only 5%, 2%, and trace amounts to the ethanolamine, inositol, and serine phospholipids, respectively. The 1-stearoyl 2-arachidonoyl species was by far the most prevalent one in the diacyl phosphatidylethanolamine (47% of total) and p...