Investigation of the Major HCV Genotype in Asymptomatic Patients in Iraq by the Use of Reverse- transcription PCR (original) (raw)
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Genotyping is considered an important tool for epidemiological and clinical studies. The biological differences between genotypes make genotyping important for decision-making regarding disease management and therapeutic intervention. Hepatitis C virus (HCV) shows a remarkable genetic diversity, contributing to its high persistence and varied susceptibilities to antiviral treatment. Nowadays, there is not enough conducted database in Saudi Arabia for having correlated pre-treatment viral load with HCV genotypes. Therefore, this study was performed to identify the prevalence of different HCV genotypes/Subtypes and pre-treatment HCV RNA viral load in HCV infected patients from selected areas of Saudi Arabia. The aim of the study is to assess Real-Time PCR as a predictor variant for a good understanding correlation between HCV genotypes prevalence, which is required for boasting improved, and sustained virological response (SVR). Data of this study was tested for correlation between HC...
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Background Nucleic acid hybridization (NAH) of hepatitis C virus (HCV) is a practical and reliable tool for virus genotyping. Genotype assignment is an important factor in the prediction of treatment success in chronic hepatitis C patients. The aim of this study was to determine the genotype distribution among HCV clinical isolates in Jordan between 2007 and 2018. Methods Electronic and paper-based clinical data registry records from 2007 to 2018 at the Jordan University Hospital (JUH) were retrospectively examined for individuals with HCV genotype, HCV viral load, and alanine aminotransferase (ALT) testing results. Genotype determination was based on NAH technique using the HCV 5′ untranslated region (5′ UTR) with 386 requests available from 342 unique individuals. Results A total of 263 out of 342 unique individuals (76.9%) had genotyping results available for final analysis with 259 individuals each having a single genotyping result. The most common HCV genotypes in the study wer...
Determination of HCV Genotypes and Viral Loads in Chronic Hepatic Sudanese infected Patients
Int J Microbiol Curr Res, 2019
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