Role of MICRORNA-155 as a Diagnostic Biomarker for Human Papillomavirus Associated Cervical Cancer (original) (raw)
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Unique microRNA expression profiles in cervical cancer
Anticancer research, 2013
Cervical cancer is the second leading cause of death among female patients with cancer in the world. Our aim was to analyze cervical cancer cases, in the Southwestern Transdanubian Region of Hungary, with regard to human papillomavirus (HPV) genotype and histological and clinical grading. After HPV testing and genotyping, the expressions of eight different pre-microRNAs (miR-21, miR-27a, miR-34a, miR-146a, miR-155, miR-196a, miR-203, miR-221) in formalin-fixed paraffin-embedded (FFPE) primary human cervical cancer samples were evaluated with the help of the LightCycler 480 PCR System (Roche). Statistically significant overexpression of miR-21 (p=0.004), miR-27a (p=0.018), miR-34a (p<0.001), miR-155 (p=0.021), miR-196a (p=0.032), miR-203 (p=0.037) and miR-221 (p=0.017) were observed in squamous cell carcinoma, regardless of HPV status and clinical grading. Significant overexpression of miR-21 (p=0.004), miR-27a (p=0.02), miR-34a (p<0.001), miR-196a (p=0.027) and miR-221 (p=0.03...
Gene Reports, 2020
Cervical cancer (CC) is the fourth most prevalent cancer worldwide. The deprivation of screenin gprogram implementation and inefficient conventional treatments of CC had led to diagnosis of disease in advanced stages and poor prognosis due to gradual increase in resistance to chemotherapy. Therefore, identification of CC molecular targets had become a primary task in order to improve detection, prognosis and development of cervical cancer targeted therapy. MicroRNAs (miRNA) have been validated as key players in cell physiology and alterations in miRNA expression were associated with cancer progression and therapy response. This study was designed to determine the expression of three microRNA molecules involved in human papilloma virus (HPV) induced cervical disease progression in Iraqi women for early detection of preneoplastic and neoplastic cervical lesions. A total of fifty cervical swab samples were collected from women who presented with cervical abnormalities and thirty samples from volunteers healthy women as a control group. Levels of miRNA expression were measured by two steps reverse transcription polymerase chain reaction (RT-PCR) technique. Results showed a significant increase in the expression levels of miRNA-21 and miRNA-20 (P = 0.0001 and 0.048, respectively) in HPV positive patients in comparison with HPV negative and healthy controls. Whereas, miRNA-143 showed a dichotomous expression pattern as being transiently upregulated (P = 0.0001) in preneoplastic lesions (CIN-II and CIN-III) and down regulated at cervical carcinoma. These data suggest that overexpression of cellular miRNA-21,-20 and-143 may be related to infection with high risk HPV and could be a biomarker and a useful therapeutic target.
Asian Pacific Journal of Cancer Prevention, 2021
Cervical cancer (CC) is categorized as the 4 th most common malignancy in women worldwide (Bray et al., 2018; Canfell et al., 2020)and nearly 90% of deaths from cervical cancer raised in low-and middle-income countries (Tsikouras et al., 2016). The annual incidence of this malignancy is more than 550,000 cases and more than 275,000 deaths in women (Arbyn et al., 2011). High risk human papillomavirus (HR-HPV) infection is distinguished as the most important etiologic factor in cervical cancer (Tian et al., 2014; Bray et al., 2018) Which causes more than 99% of this cancer cases (Nguyen et al., 2014). The E6 and E7 proteins in the HPV genome are known as viral oncogenes (Pinidis et al.
Serum and tissue miRNAs: potential biomarkers for the diagnosis of cervical cancer
Virology Journal
Background Finding new biomarkers for the early detection of cervical cancer is an essential requirement in this field. In this study, we aimed to evaluate the expression level of potential biomarkers in progression of cervical cancer in patients with cervical cancer compared to normal subjects. Methods The expression levels of tissue and serum miRNAs, including miR-9, miR-192 and miR-205, were investigated in 36 normal, 18 precancer, and 18 cervical cancer samples using real-time polymerase chain reaction. Results The results showed the higher significant expressions of miR-9, miR-192 and miR-205 in the tissue of cancer samples than those in the normal samples. Moreover, the miR-192 and miR-205 expression were significantly increased in the cancer group in comparison with the precancer group. Examination of serum samples revealed the increase in the expression level in the cancer groups than in the normal samples, for miR-9, miR-192 and miR-205 and the expressions of miR-9, miR-192...
PLoS ONE, 2011
Background: MicroRNA expression is severely disrupted in carcinogenesis, however limited evidence is available validating results from cell-line models in human clinical cancer specimens. MicroRNA-21 (mir-21) and microRNA-143 (mir-143) have previously been identified as significantly deregulated in a range of cancers including cervical cancer. Our goal was to investigate the expression patterns of several well-studied microRNA species in cervical samples and compare the results to cell line samples.
MicroRNAs as markers of progression in cervical cancer: a systematic review
BMC cancer, 2018
Invasive cervical cancer (ICC) is caused by high-risk human papillomavirus types (HR-HPVs) and is usually preceded by a long phase of intraepithelial neoplasia (CIN). Before invasion, (epi) genetic changes, potentially applicable as molecular markers within cervical screening, occur in HPV host cells. Epigenetic alterations, such as dysregulation of microRNA (miRNA) expression, are frequently observed in ICC. The mechanisms and role of miRNA dysregulation in cervical carcinogenesis are still largely unknown. We provide an overview of the studies investigating miRNA expression in relation to ICC progression, highlighting their common outcomes and their weaknesses/strengths. To achieve this, we systematically searched through Pubmed database all articles between January 2010 and December 2017. From the 24 studies retrieved, miR-29a and miR-21 are the most frequently down- and up-regulated in ICC progression, respectively. Microarray-based studies show a small overlap, with miR-10a, mi...
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, 2018
Cervical cancer is the second most common cancer in women in developing countries, including India. Recently, microRNAs (miRNAs) are gaining importance in cancer biology because of their involvement in various cellular processes. The present study aimed to profile miRNA expression pattern in cervical cancer, identify their target genes, and understand their role in carcinogenesis. Human papillomavirus (HPV) infection statuses in samples were assessed by heminested polymerase chain reaction followed by direct DNA sequencing. Next-generation sequencing and miRNA microarray were used for miRNA profiling in cervical cancer cell lines and tissue samples, respectively. MicroRNA signature was validated by quantitative real-time PCR, and biological significance was elucidated using various in silico analyses. Cervical cancer tissues samples were mostly infected by HPV type 16 (93%). MicroRNA profiling showed that the pattern of miRNA expression differed with respect to HPV positivity in cer...
Journal of Personalized Medicine
Human papillomavirus (HPV) is causatively associated with cervical cancer, the fourth most common malignant disease of women worldwide: (1) The aim of the proposed study is to implement routine diagnostics of HPV precancerous cervical lesions by introducing new molecular diagnostic tools. (2) Methods: This is a retrospective cohort study with a total of twenty-two formalin-fixed paraffin-embedded (FFPE) cervical samples of various sample type (nine biopsy and thirteen conization) each patient had a previous abnormal results of pap test or HPV DNA test. Genotyping, viral load and co-infections were determined. For each patient, the individual expression of 2549 microRNAs were evaluated by microarray and qPCR. (3) Results: Our data demonstrates that the microRNAs were commonly expressed in tissues biopsies. miR 4485-5p, miR4485-3p and miR-4497 were highly down-regulated in tissue biopsies with HPV precancerous cervical lesions. (4) Conclusions: the introduction of a microRNA analysis ...
Scientific reports, 2015
miRNAs have been established as critical layer of regulation during tumorigenesis; extracellular miRNAs are extraordinarily stable; and, quantitative reverse transcript polymerase chain reaction (qRT-PCR) provides a sensitive platform for quantifying miRNAs with a broad dynamic range. Herein, we aimed to establish a serum miRNA signature for diagnosing cervical cancer (CC). In this study, we recruited a cohort of 184 CC, 186 cervical intraepithelial neoplasia (CIN) patients and 193 healthy control subjects. qRT-PCR was performed with serum samples to screen a pool of 444 miRNAs at the initial phase, 66 miRNAs at the training phase, and 7 miRNAs at the validation phase. The profile of 4 circulating miRNAs (miR-16-2*, miR-195, miR-2861, miR-497) was established for CC diagnosis. By Receiver Operating Characteristic (ROC) curve analysis, this 4-miRNA signature showed high accuracy in discriminating CC (AUC = 0.849), and CIN individuals (AUC = 0.734) from healthy controls. Among these 4...
MicroRNA155 May Be an Important Part of Human Papilloma Virus-Related Cancers
Avicenna Journal of Clinical Microbiology and Infection
Background: Micro-RNAs (miRs) play several roles during infections with viruses. Therefore, the roles of miR21 and mir155 in the induction of the viral-related cancers have been the focus of attention in several studies. High risk human papilloma viruses (HPVs) are the main factors negatively contributing to the induction of HPV-related cancers. This study aimed to evaluate the expression of miR21 and mir155 in the patients with HPV-high risk genotypes in order to explore the roles of the miRs in the induction of HPV-related cancers. Methods: In this study, 40 women infected with the high-risk HPV genotypes as well as 40 healthy controls were examined regarding the relative expression of miR21 and mir155 by adopting real-time polymerase chain reaction (PCR) technique. U6 was used for data normalization of miRNAs. Results: Relative expressions of both miR21 and miR155 were significantly higher in the HPV-infected patients compared to those in non-infected women. Conclusions: It was c...