Isolation, identification and antimicrobial susceptibility of staphylococcus species from cattle meat slaughtered at Dukem Abattoir enterprise, East Shewa, Ethiopia (original) (raw)

2024, Research Square (Research Square)

A cross-sectional study was conducted from October 2017 to April 2018 at Dukem abattoir enterprise in order to isolate, identify Staphylococci species and determine its susceptibility to various antimicrobial agents. A total of 193 samples;110 meat swabs, 30 butcher hand swabs, 23 slaughter line swabs and 30 knives swab were collected and cultured on sheep blood agar. Presumptive staphylococci colonies were sub-cultured and primary and secondary biochemical tests were used to isolate and identify the staphylococcus species. The prevalence of staphylococcal species was 35.8% (69 out of 193 samples). Of 69 (35.8%) positive isolates, 62% were coagulase negative staphylococcus where as 37.7% coagulase positive staphylococcus. From 37.7% coagulase positive staphylococcus, S.hyicus accounted for 16% followed by S. aureus 13%, S. intermedius 9%. Antimicrobial susceptibility pattern of the isolates was done by Kirby-Bauer disk diffusion method using 13 antimicrobials. Penicillin G, Spectiomycin, Ceftriaxone, Nalidxic acid and Ceftazidime were drugs to which S. aureus, S. intermedius and S.hyicusisolates were 100% resistant. All S. intermedius was resistant to tetracycline. S aureus isolates were also to large proportion were resistant to Oxytetracycline 88.9%, Tetracycline 77.8% and PolymyxcinB 55.6%. On the contrary, all tested staphylococcus species isolates were susceptible to chloramphenicol. All S. aureus were 100% susceptible to vancomycin and doxycycline. All S.hyicus were susceptible to doxycycline. The current study revealed that the prevalence of staphylococcus species in abattoir was high and isolates of staphylococcus species were resistant to a number of drugs. Therefore, the abattoir should practice proper sanitation to avoid meat contamination, and material should be kept hygienically. In addition, further research should be done on the factor favor multidrug resistant staphylococcus species.