Pseudomonas Aeruginosa in Chronic Suppurative Otitis Media- a Drugsensitivity Study (original) (raw)
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Journal of Ayub Medical College, Abbottabad : JAMC
Chronic suppurative otitis media (CSOM) is a prevailing and notorious infection in developing countries causing serious local damage and threatening complications. Early and effective treatment based on the knowledge of causing micro organisms and their sensitivity results in good clinical recovery and prevents from damage and complications. Pseudomonas aeruginosa is most common pathogen causing CSOM in Pakistan. The objective of this study is to identify incidence of Pseudomonas aeruginosa involved in CSOM and sensitivity against commonly prescribed antibiotics. A total of 263 patients with unilateral or bilateral chronic suppurative otitis media (CSOM) were enrolled in the study at Department of ENT, Karachi Medical and Dental College & Abbasi Shaheed Hospital, Karachi, Pakistan from December 2004 to May 2006. Sterile cotton swabs were used to collect pus from discharging ears and plated on Blood agar and MacConkey agar for 24 to 48 hours. Antibiotics susceptibility was tested by ...
IntroductIon Chronic suppurative otitis media (CSOM) is a chronic inflammation (infection) of the middle ear and mastoid cavity, which presents with recurrent ear discharges or otorrhoea through a persistent tympanic membrane perforation. [1] The disease usually begins in childhood as a spontaneous tympanic membrane perforation due to an acute infection of the middle ear, known as acute otitis media, or as sequelae of less severe forms of otitis media (e.g., secretory otitis media). In general, patients with tympanic membrane perforations that continue to discharge mucoid material for periods of 6 weeks to 3 months, despite medical treatment, are recognized as CSOM cases. [2] However, the World Health Organization (WHO) definition requires only 2 weeks of otorrhoea. [3] Using the country prevalence rates as grouped based on the WHO regional classification, Nigeria is among the countries with high prevalence (2%-4%) of CSOM in the world. [4] Moreover, otitis media is the most common ear disease in the tropics and in Nigeria in particular. [5-7] CSOM is the most common otologic condition seen across otorhinolaryngology clinics in Nigeria. [8-10] In CSOM, the bacteria may be aerobic (e.g., Pseudomonas aeruginosa [P. aeruginosa], Escherichia coli, Staphylococcus Background: Pseudomonas aeruginosa has been particularly blamed for the deep-seated and progressive destruction of the middle ear and mastoid structure through its toxins and enzymes. Drug-resistant P. aeruginosa has been found to increase along with its frequency. Aim: This study aimed to assess the antibiotic sensitivity pattern of P. aeruginosa and the effectiveness of ciprofloxacin in chronically discharging ears. Patients and Methods: This was a retrospective review of 180 patients who were managed for chronic suppurative otitis media between January 2009 and December 2018. Results: Out of the 180 patients, majority 67 (37%) were in the age group 1-10 years, range of 1 month to 70 years with mean age of 18 years and standard deviation of ± 16.8 years and M: F of 1.5:1. Most have had ear discharge for more than 6 months and the majority 78 (43.4%) of the patients had bilateral disease. Central tympanic membrane perforation was the predominant finding, 81 (45.0%) with a complication rate of 21.1%. Abscess formation was the most common complication seen among the patients. P. aeruginosa was the most common bacterial isolate, 43 (23.9%) followed by Staphylococcus aureus, 39 (21.7%). In the pediatric population, P. aeruginosa was most sensitive to levofloxacin, as opposed to gentamicin in the adult population. Ciprofloxacin was seen to be more effective in adults than in children. Resistance to ciprofloxacin was seen more in the children than in adults. There was a significant association between gender and occurrence of the organism. Conclusion: P. aeruginosa is most sensitive to levofloxacin and gentamicin in the pediatric and adult population, respectively. Resistance to ciprofloxacin is most common among adults.
Spectrum of Pseudomonas Aeruginosa Sensitivity in Chronic Otitis Media
2020
Background: Chronic otitis media is perforation of tympanic membrane with infection. It is mostly seen in underdeveloped countries. Culture and sensitivity reports show that the major pathogens responsible for chronic otitis media are pseudomonas aeruginosa and staphylococcus aureus. Objective: To ascertain the sensitivity of Pseudomonas Aeruginosa in chronic otitis media which will help in the selection of antibiotics in resistant cases of chronic otitis media. Materials and Method: This prospective cross sectional study including 150 patients was carried at PNS Shifa Hospital, Karachi from 20/04/2017 to 05/01/2018.Pus sample/swab was collected via pre-packed sterile cotton sticks for bacteriology and sent to consultant microbiologist for culture and sensitivity. Laboratory reports of the bacterial spectrum and their sensitivity to antibiotics were reviewed and data entered in the proforma was analyzed. Results: After processing 36.6% pseudomonas aeruginosa infections were sensitiv...
Impact of multi drugs resistant bacteria on the pathogenesis of chronic suppurative otitis media
African Journal of Microbiology Research, 2010
One hundred twenty patients with chronic suppurative otitis media (CSOM) in Basrah, 65 (54.2%) males and 55 (45.8%) females, with male: females ratio (1.2:1) and 60 individual without otological problems as control group were included in this study, which done during the period between March 2009 and January 2010. This includes the collection of aural swab samples, culturing of samples, identification of causative agent’s species and antibiotic sensitivity. Gram’s negative bacteria were the commonest microorganism comprises (60%). Pseudomonas aeruginosa was common causative agent (19.04%), followed by Staphylococcus aureus (16.7%) andKlebsiella spp. (14.3%). Mixed infection was found in high percent (74%), in which P. aeruginosa and other microorganisms were more common. The antibiotic sensitivity pattern showed that P. aeruginosa was sensitive to Ciprofloxacin, amoxicillin +clavulanic acid and gentamicin, while other is appeared resistant, S. aureus was sensitive to ciprofloxacin, ...
Chronic suppurative otitis media(CSOM) has been a source of a tremendous health predicament since time immemorial and even today it is immensely intricate. It is an important cause of hearing impairment which in turn may cause serious long term effect on language, auditory and cognitive development and on educational development.Knowledge of the prevailing flora and their susceptibility to antimicrobials will guide the clinician as to prescribing an empirical regimen so that a better and more specific management can be provided to the patients. Hence this study is done in present secenario to isolate the organims associated with CSOM and its susceptibility pattern. To identify the bacterial and fungal profile of Chronic Suppurative Otitis Media patients. 120 patients attending ENT OPD in Navodaya Medical College Hospital and Research Centre were analysed from Nov 2009 to Oct 2010. Using predefined inclusion and exclusion criteria, samples were obtained using sterile cotton microswabs. These were cultured for microbial flora and were identified . Drug susceptibility was done using Kirby Bauer disc diffusion method.The most common organism isolated was Pseudomonas aeruginosa 33% followed by Staphylococcus aureus 25.8% among the 91 bacterial isolates. This was followed by Proteus mirabilis 20.6%, Enterobacter aerogenes and Streptococcus spp at 4.1%. The organisms were most prevalant in winter and early spring between Nov-Feb. Also maximum number of cases were seen in age group of 21-30 years.The organisms were sensitive to gatifloxacin, cefoperazone sulbactum and ceftriaxone.Fungal isolates accounted for 6.2% of the organism with Aspergillus flavus 3.1%, Aspergillus niger 2.1% and Candida albicans 1%.The study suggests that Pseudomonas aeruginosa and Staphylococcus aureus are the most common etiological agents of CSOM. Most of the strainwere sensitive to fluoroquinolones and cephalosporins.
The sensitivity pattern of ciprofloxacin and amoxicillin in chronic suppurative otitis media
Bali Medical Journal, 2022
Introduction: In both poor and developed countries, chronic suppurative otitis media affects 1-46 percent of the population. The incidence of chronic suppurative otitis media is much higher in Indonesia due to various of factors, including poor sanitation, low socioeconomic and nutritional status, and high population density. To reduce the occurrence of chronic suppurative otitis media, an adequate treatment is required. Purpose: the study was to identify the bacteria that cause chronic suppurative otitis media and ciprofloxacin and amoxicillin antibiotic sensitivity pattern in Asri Medical Centre and PKU Muhammadiyah Gamping hospital, Yogyakarta. Patients and methods: This is a prospective descriptive study with 27 patients with chronic suppurative otitis media whom met the inclusion criteria. Bacteria were isolated from ear secretions and cultured on TSA and McConkey agar medium. The antibiotics ciprofloxacin and amoxicillin were tested for susceptibility, and Gram staining, catalase test, and biochemical test were used to identify bacteria. Results: 63 bacteria were isolated from 27 chronic suppurative otitis media patients, among of them positive-gram bacteria (52%) and negative-gram bacteria (48%). The sensitivity of positive-gram bacteria and negative-gram bacteria causing chronic suppurative otitis media to ciprofloxacin was 84% and 79%, respectively. The resistance of positive-gram bacteria and negative-gram bacteria to amoxicillin by 100%. Conclusion: Staphylococcus sp and Pseudomonas aeruginosa were the most caused chronic suppurative otitis media at the Asri Medical Centre and PKU Muhammadiyah Gamping hospital in Yogyakarta. The antibiotic ciprofloxacin is the most effective treatment for bacteria that cause chronic suppurative otitis media.
International Journal of Otorhinolaryngology and Head and Neck Surgery, 2016
Background: Chronic suppurative otitis media (CSOM) is the most common middle ear infection characterized by recurrent ear discharges or otorrhoea through a tympanic perforation from the middle ear. The organisms isolated from CSOM include both aerobic and anaerobic bacteria. Among the bacteria, Pseudomonas aeruginosa (P. aeruginosa) has been particularly blamed for deep seated and progressive destruction of middle ear and mastoid structures through its toxins and enzymes. The present study was done to determine the prevalence and antibiotic susceptibility of P. aeruginosa isolated from CSOM.Methods: This was a retrospective study conducted in a tertiary care hospital in South India in March 2016. Data was obtained from the microbiology records from January 2014 to January 2016. Patients who had active ear discharge for at least three months were included in the study. P. aeruginosa was identified by colony morphology, pigment production, characteristic musty or earthy ...
https://www.ijhsr.org/IJHSR\_Vol.6\_Issue.3\_March2016/11.pdf, 2016
Introduction: Chronic suppurative otitis media (CSOM) is the most common infection of the ear characterized by recurrent, purulent discharge from the middle ear through a nonintact tympanic membrane. Its incidence has remained relatively higher in developing countries Aim/Objectives: This study was carried out in department of Microbiology, Dr.S.C.G.M.C, Nanded to determine bacteriological profile and its antibiotic susceptibility in patients with CSOM Materials & Methods: A total of 470 patientsaged 2-60 years admitted to ENT ward from July 2014 to April 2015 were studied. Ear swabs were taken and cultured aerobically on blood agar and Mac Conkeyagar. The organisms isolated were identified by standard microbiological methods and antibiotic sensitivity pattern was determined Results: Out of 470 patients, 245(52.12%) were female and 225(47.87%) were male. Peak prevalence of otitis media was documented in age group 2-10 years (39.41%). Amongst 470 ear swabs, 460(97.87%) were culture positive and 30(6.52%) were having 2 isolates. Pseudomonas aeruginosa (37.95%) was the commonest isolate followed by Staphylococcus aureus (21.83%), Coagulase Negative Staphylococcus (18.16%), Klebsiella pneumoniae (15.51%) and Escherichia coli (8.36%). Antibiotics sensitive to P. aeruginosa were Imipenem (100%), Gentamicin (65.2%) and Tobramycin (61.7%) and those resistant were Ceftazidime (71.6%) and Piperacillin (59%). Both K. pneumoniae and E. Coli species showed highest sensitivity to Amikacin (96.8%) and Ciprofloxacin (65.3%) and resistance to Amoxyclav (62.2%), Cefotaxime (61.8%) and Ceftriaxone (61.3%). Amongst Gram positive isolates, Vancomycin (100%), Clindamycin (64.3%) and Cefoxitin (53.9%) were highly sensitive and Penicillin (11.2%) was most resistant drug Conclusion: Routine use of topical antibiotics for any case of CSOM as empirical therapy must be reviewed and judicial use of antibiotics is recommended to minimise antibiotic resistance
IP Indian Journal of Anatomy and Surgery of Head, Neck and Brain
Objective: To isolate and identify various bacterial pathogens in positive ear swab cultures of CSOM cases and to determine the antibiotic sensitivity pattern of different bacterial species identified. Study design: Prospective study Setting: J. L. N. Hospital & Research Centre, Bhilai (C.G). Materials and Method: The study was done on the population of Bhilai and surrounding region. A sample size of 120 cases attending the ENT OPD with the diagnosis of Chronic Suppurative Otitis Media and active ear discharge were included in the study. Detailed history, general physical examination, local systemic examination was done along with aural swab for Gram's staining and aerobic bacterial culture and drug sensitivity. The data were then compared with the relevant & available literature. Results: Most of the patients were in the age group of 11-20 years (38.33%), followed by 21-30 years age group (31.67%). More males (60%) were affected by the disease than females (40%). Unilateral (59.16%) infection was more common than bilateral (40.83%). Incidence of monobacterial (90%) etiology was more common than polymicrobial (9.1%). Staphylococcus aureus 67 (51.53%) was the most commonly isolated bacteria followed by the Pseudomonas 26 (20%). Antibiogram showed the most effective drugs in the present study are clindamycin, cotrimoxazole, ciprofloxacin, amikacin, chloramphenicol and ceftazidime Conclusion: Staphylococcus aureus is the most common pathogen isolated, followed by Pseudomonas. Clindamycin was the most effective antibiotic followed by cotrimoxazole, ciprofloxacin, amikacin, chloramphenicol and ceftazidime. Antibiotic sensitivity of the organisms varies according to geographical area and local practice regarding the choice of antibiotics. A continuous and periodic evaluation of microbiological pattern and their antibiotic sensitivity pattern in local area is helpful in prescribing empirical antibiotics for successful treatment of otitis media and thus minimizing its complications and emergence of resistant strains.
Antibiotics susceptibility of bacterial pathogens associated with otitis media
African Journal of Bacteriology Research, 2010
One hundred twenty patients with chronic suppurative otitis media (CSOM) in Basrah, 65(54.2%) males and 55(45.8%) Females, with male: females ratio (1.2:1) and 60 individual without ontological problems as control group were included in this study, which was done during the period of March, 2009 to January, 2010. This Include collection of aural swab samples, culturing of samples, identification of causative agents species and antibiotic sensitivity. Gram ' s negative bacteria were the commonest microorganisms; it comprises (60%). Pseudomonas aeruginosa was common causative agent (19.04%), followed by Staphylococcus aureus (16.7%) and Klebsiella spp. (14.3%). Mixed infection was found in high percent (74%), in which P. aeruginosa and other microorganisms were more common. The antibiotic sensitivity pattern showed that P. aeruginosa was sensitive to Ciprofloxacin, amoxicillin +clavulanic acid and gentamicin, while other is appeared resistant, S. aureus was sensitive to ciprofloxacin, amoxicillin+clavulanic acid, erthomycin, cephalexine and it is resistant to pencillin and ampicillin, klebsiella species were sensitive to ciprofloxacin, amoxicillin +clavulanic acid ,gentamicin,while resistant to tetracycline.