Effect of caffeic acid phenethyl ester on bone formation in the expanded inter-premaxillary suture (original) (raw)
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Effect of Energy Drink on Bone Formation in the Expanded Inter-Premaxillary Suture
Journal of Craniofacial Surgery, 2017
Maxillary expansion of the median palatal suture is a common procedure in orthodontics. Even after retention, there is a strong tendency to relapse in the expanded suture. The authors' objectives are to accelerate the bone formation process in the expanded suture and to reduce the required retention time by using an energy drink (ED). Twenty rats were divided into 2 groups (n ¼ 20). The expansion-only group was defined as the control group (Group C). The other group was defined as the expansionplus-energy drink group (Group ED). In Group ED, ED was administered systemically through oro-gastric tubes after the expansion period. After 5 days of expansion, the springs were removed and replaced with short lengths of rectangular retaining wire. Tooth separation was maintained for 12 days. Histomorphometric analysis showed significant differences between the 2 groups in terms of newly formed bone (P ¼ 0.018) and the bone area (P ¼ 0.007). For the parameters that were investigated, Group ED had better results than Group C. These results show that systemic administration of an ED during the early stages of the orthopedic expansion of the intermaxillary suture areas can stimulate bone formation and decrease the time required for retention.
Effect of Grape Seed Extract on Bone Formation in The Expanded Inter-premaxillary Suture
Meandros Medical and Dental Journal, 2020
Original Article / Özgün Araştırma Meandros Med Dent J 2019 Öz Amaç: Median suturun maksiller genişletilmesi ortodonti sıklıkla yapılan bir uygulamadır. Retansiyon sonrasında bile genişlemiş suturda geriye gidiş görülebilmektedir. Amacımız bu suturdaki genişlemedeki kemikleşmeyi hızlandırmak ve kemikleşmeye etkisi olan üzüm çekirdeği ekstresi kullanarak retansiyon süresini kısaltmaktır. Gereç ve Yöntemler: Yirmi adet rat iki gruba ayrıldı. Kontrol Grubuna sadece ekspansiyon yapıldı. Deney grubuna ise ekspansiyona ilaveten üzüm çekirdeği ekstresi verildi. Deney grubuna ekspansiyon sonrası orogastrik yöntemle sistemik olarak ekstreler verildi. Maksillaya springler uygulanıp aktiflendi. 5 gün sonra springler çıkartıldı ve köşeli tellerle retansiyon yapıldı.12 gün pekiştirme yapıldı. Objective: Expansion of the median palatal suture is a common procedure in orthodontics. Even after retention, there is a strong tendency to relapse in the expanded suture. Our objectives were to accelerate the bone formation processes in the expanded suture and to reduce the required retention time by using grape seed extract (GS), which can potentially stimulate bone formation. Materials and Methods: Twenty rats were divided into two groups (n=20). The expansion only group was defined as the control group (Group C). The other group was defined as the expansion plus grape seed extract group (Group GS). In Group GS, GS was administered systemically via orogastric tubes after the expansion period. The springs were placed and activated to deliver a force. After 5 days, the springs were removed and replaced with short lengths of rectangular retaining wire. Tooth separation was maintained for 12 days. Results: We observed significant differences in the percentage of newly formed bone (p<0.05) and bone area (p<0.05) between the two groups. For the parameters investigated, Group GS had better bone formation than Group C. Conclusion: Systemic administration of GS during the early stages of the orthopedic expansion of the inter-premaxillary suture areas can stimulate bone formation and decrease the time needed for retention.
Effect of Royal Jelly on new bone formation in rapid maxillary expansion in rats
Medicina Oral Patología Oral y Cirugia Bucal, 2015
Background: The aim of this study was to evaluate the effects of long and short term systemic usage of royal jelly on bone formation in the expanded maxillary suture in a rat model. Material and Methods: Twenty eight Wistar albino rats were randomly divided into 4 equal groups: Control (C); Only Expansion (OE), Royal Jelly (RJ) group, Royal Jelly was given to rats by oral gavage only during the expansion and retention period; Royal Jelly plus Nursery (RJN) group, Royal Jelly was given to rats by oral gavage during their nursery phase of 40 days and during the retention period. After the 5 day expansion period was completed, the rats underwent 12 days of mechanical retention. All rats were sacrificed in same time. Histological examination was performed to determine the number of osteoclasts, number of osteoblasts, number of capillaries, inflammatory cell infiltration, and new bone formation. Results: New bone formation, number of osteoclasts, number of osteoblasts, and the number of capillaries in the expanded maxillary sutures were higher in the RJ and RJN groups than in the other groups. Statistical analysis also demonstrated that new bone formation and the number of osteoblasts was also highest in the RJN group. Conclusions: The systemic administration of Royal Jelly in conjunction with rapid maxillary expansion may increase the quality of regenerated bone.
Effect of salvianolic acid B on new bone formation in the orthopedically expanded suture
The Angle Orthodontist
Objectives To determine the effects of Salvianolic acid B (Sal B) on new bone formation in the orthopedically expanded premaxillary sutures in rats. Materials and Methods The sample consisting of Sprague Dawley rats (male, n = 14) was split in half by random selection: the experiment group (Sal B) and the control group. The premaxillary suture of each rat was expanded by bonding an open-loop spring to two maxillary incisors, each end to one tooth. A 5-day expansion period followed by a 12-day retention period was conducted. The 17-day intraperitoneal administration of Sal B was performed daily for the experiment group at a dose of 40 mg/kilo. The trial was completed after sacrificing the rats and dissection of the premaxillae for histological analysis. The amount of new bone, quantity of capillaries and intensity of inflammatory cells were histomorphometrically determined while the quantities of osteoblasts and osteoclasts were determined immunohistochemically. Results The Sal B gro...
Effects of lactoferrin on bone resorption of midpalatal suture during rapid expansion in rats
American Journal of Orthodontics and Dentofacial Orthopedics, 2018
The aim of this study was to investigate the effect of lactoferrin (LF) on bone resorption of rats' midpalatal sutures during rapid palatal expansion. Methods: Sixty male 5-week-old Wistar rats were randomly divided into 3 groups: expansion only (EO), expansion plus LF (E 1 LF), and sham device (control). Results: Microcomputed tomography showed that the bone volume/tissue volume ratio and the relative bone mineral density of the suture bone were significantly increased in the E 1 LF group compared with the EO group. Histochemical staining suggested that the activity of osteoblast-like cells and the amount of new bone formation were stimulated in the E 1 LF group whereas the activity of osteoclasts showed no obvious difference between groups. On the other hand, the immunohistochemical and the real-time polymerase chain reaction results showed that the expressions of receptor activator of nuclear factor kappa B ligand and osteoprotegerin had no significant difference between the EO and E 1 LF groups. Conclusions: These findings demonstrated that LF could stimulate bone volume and bone density in midpalatal sutures during the suture remodeling process under tensile force. However, this enhancement effect was not caused by the reduction of bone resorption.
Effects of teriparatide on bone formation in rats with experimentally induced premaxillary expansion
Dental Press Journal of Orthodontics
Objective: This study aimed to evaluate the effects of systemic teriparatide on sutural bone formation after premaxillary suture expansion in rats. Material and Methods: Twenty Wistar male rats (8-10 weeks old) were randomly divided into two groups, namely, control (C, n=10) and teriparatide (T, n=10). An expansion force was applied to the maxillary incisors using helical spring for a seven-day expansion period, for both groups. On the eighth day, the rats were kept for a seven-day consolidation period, and then 60 µg/kg teriparatide (once a day) was administered to group T subcutaneously for seven days. Then, all the rats were sacrificed, and histological sections were stained with hemotoxylin-eosin for examination. Anti-osteonectin, anti-osteocalcin, anti-Vascular endothelial growth factor (VEGF) and anti-transforming growth factor beta (TGF-β) were evaluated by immunohistochemical analysis in the midpalatal suture area. Results: Histologically, the newly formed bone tissue was ob...
Orthodontics & Craniofacial Research, 2011
Objective-The aim of this study was to evaluate the effects of local resveratrol (RSVL) administration on bone formation in response to expansion of the interpremaxillary suture, in rats. Material and Methods-Twenty 50-to 60-day-old male Wistar rats were separated into two equal groups. Both groups were subjected to expansion, and 30 cN of force was applied to the maxillary incisors with helical-spring. Twenty-four hours after appliance placement, single-dose 10 lmol ⁄ kg RSVL in the dimethylsulfoxide (DMSO) was injected to the inter-premaxillary suture in the experimental group. In the control group, the same amount of DMSO was injected to the suture of rats. Bone formation in the suture was evaluated histomorphometrically. The area of new bone (lm 2), the perimeter around the new bone (lm), FeretÕs diameter (lm), the percentage of new bone to non-ossified tissue (%), and the number of osteoblast were measured and compared. Mann-Whitney U-test was used for statistical evaluation at p < 0.05 level. Results-Statistically significant differences were found between the groups for all histomorphometric parameters. New bone area (p < 0.001), bone perimeter (p < 0.001), FeretÕs diameter (p < 0.001), percentage of new bone (p < 0.001), and the number of osteoblast (p < 0.001) were significantly larger in the experimental group when compared with the control. Bone histomorphometric measurements revealed that bone architecture in the RSVL treated rats was improved. Conclusions-Local application of RSVL during the early stages to orthopedically expanded inter-premaxillary suture area may stimulate bone formation and shorten the retention period, in rats.
Comparison of Dentofacial and Periodontal Effects of Rapid and Semi-rapid Maxillary Expansion
Meandros Medical and Dental Journal, 2017
Objective: The purpose of this study was to compare the skeletal, dentoalveolar and periodontal effects that occurred after rapid maxillary expansion (RME) and semirapid maxillary expansion (SRME) with an acrylic splint bonded expansion appliance and also to compare the degree of root resorption in the supporting teeth during expansion. Materials and Methods: The sample of the study included 19 patients having maxillary constriction requiring bilateral upper premolar extraction. The semi-rapid group consisted of 10 patients and the rapid group consisted of 9 patients. Lateral and posteroanterior (PA) cephalometric radiographs were taken at the beginning of the treatment, at the end of expansion and after 3 months of retention period. Eleven patients underwent low-dose computed tomography (CT) at the beginning of treatment and at the end of retention period, after giving informed consent. The evaluation of root resorption was made using a scanning electron microscope from the extracted teeth. Results: Both lateral and PA radiographs showed similar changes between the groups. Posterior nasal cavity width, palatal maxillary width, the distance between palatinal roots and the distance between incisor roots showed significant increase in the CT measurements. Buccal bone thickness decreased and palatal bone thickness increased in all molar and premolar teeth examined. Root resorption was seen in both groups, but no significant differences were observed between the groups. Conclusion: Some differences were seen in short-time effects between SRME and RME but these differences were temporary and disappeared at the end of the retention period. The qualitative evaluation of the roots showed that the location and degree of the root resorption were similar between the groups. Amaç: Bu çalışmanın amacı, akrilik splintli bonded ekspansiyon apareyi ile yapılan yarı hızlı maksiller ekspansiyon (SRME) ve hızlı maksiller ekspansiyon (RME) ile meydana gelen iskeletsel, dentoalveolar ve periodontal etkileri; ayrıca tedavi sırasında destek dişlerde meydana gelen kök rezorpsiyonunun şiddetini karşılaştırmaktır. Gereç ve Yöntemler: Maksiller darlığa sahip ve üst 1. premolar çekimi planlanan 19 birey çalışma grubunu oluşturmuştur. SRME grubunu oluşturan 10 birey ve hızlı
European Journal of Dentistry
Objectives To investigate the effect of caffeic acid phenethyl ester (CAPE) provision on matrix metalloproteinase-9 (MMP-9), fibroblast growth factor-2 (FGF-2) expression, osteoclast and osteoblast numbers during experimental orthodontic tooth movement (OTM) in male Wistar rats (Rattus norvegicus). Materials and Methods Forty-eight healthy male Wistar rats (R. norvegicus), 16 to 20 weeks old with 200 to 250 g body weight (bw) were divided into several groups as follows: K1: OTM for 3 days; K2: OTM for 7 days; K3: OTM for 14 days; KP1: OTM and CAPE for 3 days; KP2: OTM and CAPE for 7 days; and KP3: OTM and CAPE for 14 days. A nickel titanium closed coil spring 8.0 mm long with 10 g/mm2 was installed between the upper left first molar and upper central incisor to move molar mesially. CAPE provision with a dose of 20 mg/kg bw of animal studies was done per orally. Immunohistochemistry was done to examine MMP-9 expression and osteoclast number in compression side as well as FGF-2 expres...