The study iron, zinc, copper and magnesium in hydatid cyst Fluid in Kerbala City (original) (raw)
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Science Journal of University of Zakho, 2014
This study included comparative biochemical composition of hydatid fluid, protoscolices, infected and noninfected tissues isolated from liver and lungs of infected sheep, goats, and cattle in Duhok abattoirs during the period from Nov. 2009 to Apr. 2010. Also hydatid fluid of cysts surgically removed from humans in Azadi Teaching Hospital, Duhok during the period from Mar. 2010 to Jul. 2010.Hydatid cysts and host tissues were analyzed for Ions (Na + , K + , Ca ++ , and Mg ++) and Fe ++. Among Ions, Na+ exhibited high levels in hydatid fluid of the studied hosts with the highest being in hydatid fluid of sheep liver cyst (356±8.207 mg/dl); furthermore, infected tissues showed higher Na + levels with the highest being in sheep liver and lung tissues (196±7.461 and 178±5.868 mg/100g respectively). Protoscolices of both liver and lungs showed high K + levels, among tissues, infected tissue contained high K + levels with the highest being in infected lung tissues (Ranged from 63.46±0.597 mg/100g to 77.39±0.729 mg/100g). Nearly similar levels of Ca ++ were detected in hydatid fluid and protoscolices of all cysts with the highest level being in goats cysts protoscolices (Liver: 9.212±0.081 mg/100g, Lungs: 9.044±0.072 mg/100g) and the lowest in cattle cysts fluid (Liver: 6.954±0.061 mg/dl, Lungs: 6.826±0.054 mg/dl). The level of Mg ++ was low in hydatid cysts and tissues of all hosts (Ranged from 0.746±0.016 mg/100g to 0.952±0.015). Small quantities of iron were detected in hydatid cysts of all hosts which were higher in cattle liver cysts (Hydatid fluid 0.0882±0.003 mg/dl and protoscolices 0.0838±0.003 mg/100g) and infected liver tissues (0.2293±0.008 mg/100g).
Kurdistan Journal for Applied Research
Hydatid disease is a helminthic infection, caused by the metacestode stage of cestode belonging to the Echinococcus grnulosus (dog tapeworm). The species and strain identification of Echinococcus are of medical importance for strategic control measuring. The present study achieved to determine and compare fertility and biochemical profiles of hydatid cyst fluid isolated from human, cattle, sheep and goat. The human originated cyst has been found to be more fertile (88%), followed by sheep-originated cyst (85%). The high rate of fertility was found in the liver followed by lungs in all intermediate hosts. However, the statistically significant differences of a biochemical test of cyst fluid among intermediate host were observed just in the uric acid, while the result of biochemical tests Hydatid cyst fluid isolated from liver significantly was higher than that of the lung for the most biochemical tests. The fertility and biochemical parameters value differences directly have related...
Background and objectives: Hydatidosis is an important zoonotic disease with widespread distribution. For unknown reasons, some cysts are unable to produce protoscoleces, and little is known about the mechanisms involved in infertile cyst production. Therefore, characterization of hydatid cyst fluid (HCF) components could help clarify the host-parasite relationship and the fertility process of cyst. The aim of this study was to identify and quantify biochemical components of HCF of fertile and infertile cysts from different hosts. Methods: A total of 35 HCF samples were obtained from the liver and lung of 16 cattle, 16 sheep and three humans. Fertility of cysts was determined by examination of cysts' content. Then, total fluid was aspirated aseptically from each cyst. The samples were centrifuged at 10000×g for 15 min at 4°C, and then concentrated and dialyzed against phosphate buffer saline using an Amicon Ultra-15 5000 MWCO centrifugal filter device (Millipore, USA). All biochemical components were quantified by an automatic analyzer. Results: The value of lactate dehydrogenase, potassium, calcium, cholesterol, glucose, urea and uric acid differed significantly in cysts collected from different hosts (P<0.001). There was also a significant difference in the amount of lactate dehydrogenase, aspartate aminotransferase and cholesterol between fertile and infertile cysts (P<0.039). Conclusion: Due to the differences in biochemical composition of HCF of different host origin, it is possible that the host plays a crucial role in determining the type of biochemistry in hydatid cyst as well as in hydatid cyst fertility.
2014
Cystic hydatid disease caused by the larval stage of a taeniid cestode Echinococcus granulosis is one of the most important cosmopolitan parasitic zoonoses. The aim of the present study was to compare the fertility, the viability and some biochemical parameters in hydatid cyst fluids obtained from the liver and lungs of sheep naturally infected with hydatidosis. Lungs were found to be more fertile (69%), followed by liver (65%). The proportion of viable protoscoleces from fertile cysts was 85% and 89% from lungs and liver, respectively. However, no significant differences were reported. This study showed also different means of glucose, triglycerides, cholesterol, calcium, potassium, sodium and chlorine in hydatid fluids from the liver and lungs. Generally, no significant differences in biochemical profiles were observed in sheep. The results of this study showed that a majority of cysts in sheep were fertile with higher proportion of viable protoscoleces in lungs and liver indicati...
A descriptive, parasitological, and biochemical study done on three patients with hydatid disease aged 9, 11and 13 years attending Tikrit Teaching Hospital for the period from first of January to the 20th of September 2007. Two of the study cases undergo surgery and the sample of hydatid cyst fluid were examined parasitologicaly and biochemically. All the parameters above were compared with that of the adults cattle and sheeps with hydatid disease. Two are females and one male. All the patient from the urban area. Two patients have cough and shoerness of breath as the main complaint while the other pateint have no complaint. Two patients have both hepatic and lung cysts and the other one has single hepatic cyst. All the hepatic cysts were in the right lobe. There is no relation between the age of the patient and the size of hydatid cyst. The highest infectivity was in the hydatid fluid from the sheep (90%) while the lowest was from the cattle (20%). Infectivity in human both children and adults was 30%. The results of biochemical parameters of cyst fluids from various hosts shows that he quantity of uric acid was found to be significantly more in human cyst fluids compared with those from other species. The level of proteins, glucose, triglyceride and creatinin are lower in cysts of human (children and adults) compared to that of sheeps and cattle.
A cross-sectional survey of bovine hydatidosis was conducted on cattle and sheep slaughtered at Mizan Aman city administration Municipal Abattoir from April, 2019 to June, 2019 to determine the prevalence and cyst characterization caused by hydatid disease. No statistical significant variation was observed with regards to place of origin and age of the animals [P > 0.05]. After postmortem examination, hydatid cysts were collected and cyst characterization was conducted. Out of a total of 438 animals examined, 41[10.68%] harboring one or more hydatid cyst. The result obtained from Postmortem examination indicated that the lung was the most commonly affected organ followed by liver. From the total of 41 cysts counted, 9[21.95%], 15[36.58%], 17[41.46%] were large, medium and small respectively and 6[14.63%], 30[73.17%] and 5[12.19%] were fertile, sterile and calcified respectively. Out 6 of fertile, 16% were viable, all from the lung and higher liver calcification were observed.
Comparative Clinical Pathology, 2017
This study was performed to evaluate the electrolytes and biochemical profiles in hydatid cyst fluid (HCF) collected from 60 slaughtered animals (dromedary camels, goats and cattle) in Oman. The Beckman Coulter Chemistry Analyser was used to analyse the HCF aspirated from 30 fertile and 30 sterile cysts collected from livers and lungs. The results revealed that the means of sodium, calcium, glucose, magnesium, creatinine and total protein in dromedary camels, cattle and goats varied significantly (p < 0.05) as compared to their normal serum ranges. Based on the location of cyst, significant (p < 0.05) variations in the means of sodium, glucose in cattle, glucose and magnesium in camels and chloride in goats were observed in HCF of livers and lungs (p < 0.01). The comparison of biochemical composition between fertile and sterile HCF revealed significant difference between the means of sodium (p = 0.003) and chloride (p = 0.012) in camels and phosphorus (p < 0.01), triglycerides (p = 0.015) and total protein (p = 0.008) in cattle, while the means of magnesium (p < 0.01), phosphorus (p < 0.01), creatinine (p < 0.01) and total protein (p = 0.002) varied in fertile and sterile HCF of the goats. These differences in biochemical components of HCF from various intermediate hosts could be related to the variations in parasite metabolism, growth rate of the larvae or strain variations. Further investigations are required to compare these results with the HCF collected from human patients, which could be used for the diagnostic purpose and stain identification.
Echinococcosis, a worldwide zoonosis, is caused by the larval stages of Echinococcus granulosus and E. multilocularis belonging to the family taeniidae. Because of the increasing consumption of red meat; survey, prevention and treatment of this disease will be essential. Also detection of contaminant factors including parasitic agents and the factors that are related to the hosts is helpful to codify a useful programme for prevention of this disease. This slaughterhouse based survey has been done to determine the rate of hydatidosis in Ilam province on 2380 sheep, 975 goats and 1820 cows. Overall 10% of infected livers and lungs from each animal group randomly collected and the number of cysts was determined macroscopically. The rate of hydatidosis was 6.8 % in sheep, 4.2 % in goats and 8.6 % in cows. The infection rates of lungs in sheep, goats and cows were 82, 73 and 75%, respectively, while the infection rates of livers were 12, 8 and 13%, in sheep, goats and cows, respectively. The difference of infection rates between lung and liver was statistically significant (p<0.05). After transferring of specimens to the laboratory for detection of protoscolices, results shows that the rate of alveolar cysts were teemed in sheep, cows and goats were 12 %, 24 % and 22 %, respectively and also exuberated hepatic cysts in sheep, cows and goats were 25 %, 2.5 % and 18 %, respectively. The rates of alive protoscolices in fertile cysts were determined by eosin staining and the percentage of protoscolices that were stained were considered alive. Their cumulative rates were 79.45, 88.95 and 72.02 % while their rates in livers were 65.71, 38.45 and 86.14% in sheep, cows and goats, respectively. These results showed a considerable infection of sheep and goats in this region. It could be concluded that because sheep, goats and guard dogs are maintained close to together, therefore these high rates of infection are due to continuous contact of these animals with dogs and their feces. Also low knowledge of people about true prevention strategies especially in the deprived region that has nomadic inhabitants may be the main cause of increasing of hydatid cyst infestation. The prevention strategies like suitable annihilation of infected viscera would be key task in decreasing hydatid cyst rate in Ilam.
International Journal for Agro Veterinary and Medical Sciences, 2014
A total of 987 carcasses of sheep (459) and goats (528) of different ages, in the period of June, 2012-June, 2013, reared in different localities at El-Kharga city (in and out the abattoir), where 37 (8.06%) of sheep and 29 (5.49%) of goats were found harbored hydatid cyst liver, and lung were the most infected organ in sheep at rate of 4.57% and 2.61%, respectively, but in goats the lung was the most predilection site than liver in rate of 3.03% and 2.46% respectively, both liver and lung were cystic, in sheep only in rate of 0.65%, and the under develop cyst recorded in kidney of sheep 0.28%. Sheep fell in age category of 3 year and above were more infected (15.29%) compared to 1 to 3 year (4.23%) and below 1 year (0%) old. Likewise, the prevalence in goats was 7.89%, 3.79% and 0% respectively in the same age groups. Fertility of cyst was highest in sheep than goats in rate of 62.16% and 58.62%, respectively. The biochemical analysis revealed significant increase in protein level of lung cysts extract (P < 0.01) than liver cyst extract in sheep and goats. While liver cyst extract revealed significant increase in both of, creatinine (P < 0.01), calcium ions (P < 0.01) and magnesium ions (P < 0.05) than lung cyst extract in both sheep and goats, while cholesterol and triglyceride revealed significant increase in liver cyst extract of sheep and no change in liver cyst extract of goats. The differences in chemical component values of cyst extract in both sheep and goat and cystic organ in the same animal may pointed some strain variability in parasite metabolism, growth rate or even strain variation.