Bacteriostatic and antidiabetic activities of various extracts from the mixture leaves and stems of Suaeda marimita (original) (raw)
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Journal of Babol University of Medical Sciences, 2023
Background and Objective: Control of hyperglycemia after meals is an important strategy in the management of type II diabetes, and the reduction of chronic complications associated with it. Therefore, inhibitors of carbohydrate-degrading enzymes such as α-glucosidase can be useful in the treatment of this disease. The aim of this study was to evaluate the α-glucosidase inhibitory activity in some traditional medicines in Sulaymaniyah province of Iraq. Methods: Eight plant species were prepared with advisory from authentic spiceries in Sulaymaniyah. After air drying and preparation of their methanolic extracts, enzyme microplate assay was conducted on them in four different extract concentrations (1, 0.1, 0.01 and 0.001 mg/ml) along with negative and positive controls. Finally, enzyme kinetic analysis was performed on effective extracts. Findings: Among the eleven studied plant species, Rubus idaeus L., Rheum ribes R. and Salix alba L. extracts at 1 mg/mL concentration showed highest inhibitory activity (98.13±1.05% (IC50=0.046 mg/mL), 93.39±2.11% (IC50=0.085 mg/mL) and 84.46±1.12% (IC50=0.125 mg/mL), respectively) against α-glucosidase,. After kinetic analysis of α-glucosidase inhibition, Rubus idaeus L., Rheum ribes R. extracts showed mixed type of inhibition (competitive- uncompetitive) while extract of Salix alba showed uncompetitive type of inhibition. Conclusion: The leave extract from both Rubus idaeus, Salix alba and root extract of Rheum ribes are remarkable α-glucosidase inhibitors, and may be used in the treatment of type II diabetics after clinical tests.
Phytochemical Analysis, Antidiabetic Potential and in-silico Evaluation of Some Medicinal Plants
Pharmacognosy Research, 2021
Background: The increasing frequency of diabetes patients and the reported side effects of commercially available anti-hyperglycemic drugs have gathered the attention of researchers towards the search for new therapeutic approaches. Inhibition of activities of carbohydrate hydrolyzing enzymes is one of the approaches to reduce postprandial hyperglycemia by delaying digestion and absorption of carbohydrates. Objectives: The objective of the study was to investigate phytochemicals, antioxidants, digestive enzymes inhibitory effect, and molecular docking of potent extract. Materials and Methods: In this study, we carry out the substratebased α-glucosidase and α-amylase inhibitory activity of Asparagus racemosus, Bergenia ciliata, Calotropis gigantea, Mimosa pudica, Phyllanthus emblica, and Solanum nigrum along with the determination of total phenolic and flavonoids contents. Likewise, the antioxidant activity was evaluated by measuring the scavenging of DPPH radical. Additionally, antibacterial activity was also studied by Agar well diffusion method. Molecular docking of bioactive compounds from B. ciliata was performed via AutoDock vina. Results: B. ciliata, M. pudica, and P. emblica exhibit significant inhibitory activity against the α-glucosidase and α-amylase with IC 50 (µg/ml) of (2.24 ± 0.01, 46.19 ± 1.06), (35.73 ± 0.65, 99.93 ± 0.9) and (8.12 ± 0.29, no significant activity) respectively indicating a good source for isolating a potential drug candidate for diabetes. These plant extracts also showed significant antioxidant activity with the IC 50 ranges from 13.2 to 26.5 µg/mL along with the significant antibacterial activity towards Staphylococcus aureus and Klebsiella pneumonia. Conclusion: Bergenia extract appeared to be a potent α-glucosidase and α-amylase inhibitor. Further research should be carried out to characterize inhibitor compounds.
The Pharma Innovation Journal, 2019
An in vitro anti-diabetic activity of various indigenous medicinal plants was evaluated by α-amylase and α-glucosidase inhibition assay methods. Various extracts like chloroform, methanol and water extracts have been prepared by simple maceration of defatted plant powder using n-hexane. Qualitative phytochemical analysis was performed to evaluate the presence of active ingredients like flavonoids, alkaloids, glycosides, saponins, steroids, carbohydrates and tannins. Chloroform extracts of Gymnema sylvestre (Retz.) R.Br. ex Sm leaf, Lepidium sativum L. seed and Pueraria tuberosa (Willd.) DC. Tuber exhibited significantly higher (p < 0.05) inhibition of α-amylase activity as compare to standard drug acarbose. Water extracts of Solanum xanthocarpum Schrad. & H. Wendl. Leaf and Centratherum anthelminticum (L.) Gamble seed shown significantly higher (p < 0.05) inhibition of α-glucosidase followed by methanolic extracts of Pueraria tuberosa (Willd.) DC. Tuber and Moringa oleifera La...
Journal of Herbmed Pharmacology
Introduction: α-Glucosidase is the major enzyme implicated in intestinal glucose absorption, and its inhibition is a target for the management of diabetes mellitus. This study investigated the in vitro α-glucosidase inhibitory activity of extracts from different parts of 20 selected medicinal plants and the potential for plant-part substitution and plant species combinations used by traditional healers to treat diabetes. Methods: Acetone and petroleum ether extracts from different parts of 20 plant species traditionally used to treat diabetes were individually evaluated in vitro using an α-glucosidase assay. The potential for plant-part substitution was investigated by including leaf extracts where non-renewable parts are used traditionally. The extracts of plant species were combined and investigated as used traditionally. Results: Anthocleista grandiflora stem bark acetone, Artabotrys brachypetalus leaf petroleum ether, and Dichrostachys cinerea root petroleum ether extracts exhib...
EVALUATION OF SELECTED MEDICINAL HERBS FOR ANTIDIABETIC ACTIVITY VIA ALPHA-GLUCOSIDASE INHIBITION
Literature and native therapies have cited bitter melon, dandelion, blueberry, and roselle, as hypoglycemic agents, however, the exact mechanisms of action are unknown. It was hypothesized that, these agents could induce hypoglycemia, through the mechanism of α-glucosidase inhibition. The aim of the present study was, to examine inhibition of alpha-glucosidase as one of the possible mechanisms of action, of bitter melon (Mormodicacharantia), dandelion (Taraxacumofficinale), blueberry (Vacciniumcorybosum), and roselle (Hibiscus sabdariffa). Each of these agents has been used in the treatment of diabetes in, different parts of the world. The study was done in vitro, using α-glucosidase, obtained from Bacillus. The inhibitory effect of different concentrations of alcoholic extracts of the plants, on α-glucosidase was studied. The extracts of the plant showed inhibitory activities, against α-glucosidase, with IC50 values in a dose dependent manner. The result demonstrated that, bitter melon, roselle, dandelion, and blueberry share similar mechanism of action with Acarbose, which is being used as an antidiabetic agent.
Biolife, 2023
Diabetes mellitus is a metabolic condition marked by high blood glucose levels as well as changes in carbohydrate, lipid, and protein metabolism. The goal of this study was to determine the inhibitory effect of selected plants (Artemisia vulgaris-root, and Angelica archangelica-leaves) on-glucosidase and-amylase to assess anti-diabetic effectiveness in vitro. By using the Soxhlet extraction method, each plant powder was repeatedly extracted with different organic solvents of increasing polarity. To investigate invitro anti-diabetic efficacy, the various solvent extracts were submitted to a-glucosidase and-amylase enzyme inhibition assay. Artemisia vulgaris stem bark crude extracts yielded 22.43 percent, 25.56 percent, 10.14 percent, 12.12 percent, and 1.10 percent in hexane, chloroform, ethyl acetate, acetone, and methanol, respectively. Angelica archangelica extracts were shown to have IC50 values of 57 g/ml, 43 g/ml, 63 g/ml, 64 g/ml, and 70 g/ml in n-hexane, chloroform, ethyl acetate, acetone, and methanol, respectively. In a dose-dependent way, all extracts reduced enzyme activity. Based on the IC50 values, Angelica archangelica chloroform extract was the most active of the two plant species, followed by Artemisia vulgaris. The chemicals responsible for A. archangelica promise in vivo anti-diabetic effect should be studied further.
In vitro biological effects of two anti-diabetic medicinal plants used in Benin as folk medicine
BMC Complementary and Alternative Medicine, 2013
Background: Extracts from Polygonum senegalensis (Polygonaceae) and Pseudocedrela kotschyi (Meliaceae) are two important traditionally used medicinal plants in rural Benin to treat many diseases and notably type 2 diabetes. The aim of the study was to investigate the α-glucosidase inhibition, antioxidant and antibacterial activities of those plants extract: Polygonum senegalensis leaves, and Pseudocedrela kotschyi root. Methods: Hydro-alcoholic (50%) extracts were analyzed for their phytochemical content and tested for their inhibition potency on α-glucosidase from Saccharomyces cerevisiae. Antioxidant activities were assessed using the DPPH, ORAC, FRAP and DCFH-DA (cell based) assay. Finally, the antibacterial activity was evaluated using MIC determination on four Gram-positive cocci (Bacillus subtilis, Clostridium difficile, Enterococcus faecalis, Staphylococcus aureus), three Gram-negative bacilli (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae), and the yeast Candida albicans.
Journal of Developing Drugs, 2015
Inhibition of α-glucosiase and the associated reduction of glucose absorption is an attractive approach for decreasing postprandial hyperglycemia and for the discovery of potent antidiabetic agents. One of the most important sources of potential α-glucosiase inhibitors represents the class of polyphenols. This paper aims to evaluate previous herbal polyphenol-rich extracts plan on the management of diabetes mellitus, to address their α-glucosidase inhibitory activity. Polyphenol-rich extracts from thirteen widely used traditionally anti-diabetic plants in Asia and Mediterranean regions were evaluated for their potential α-glucosidase inhibitory activity. Among these evaluated plants, 10 were much stronger than that of acarbose standard. Punica granatum manifested the highest inhibitory activity with IC 50 at 3.59 ± 0.11 µg/mL, followed by Psidium guajava with IC 50 at 8.08 ± 0.10 µg/mL and Cinnamomum zeylanicum with IC 50 at 9.87.08 ± 0.14 µg/mLA. A high correlation (r=0.65, p<0.001) was observed between α-glucosidase inhibition and total phenolic content of all plants. Punica granatum, P. guajava, C. zeylanicum and Ziziphus spina-christi had also the highest total phenolic content. Extracts for the above studied plant species may potentially replace acarbose in its current clinical use in improving post-prandial glycaemic control in type 2 diabetics. As a result, these polyphenol-rich extracts potentially offer a complementary approach to develop functional food and potential antidiabetic agents.
2024
The antidiabetic and antioxidant efficacy of traditional medicinal plants from NorthEastern India remains largely unexplored. This study seeks to address this gap by examining the properties of 11 such plants. The plant samples were subjected to extraction using four different solvents. Their antidiabetic potential was evaluated through in-vitro α-glucosidase and α-amylase inhibition bioassays, while the antioxidant activity was assessed using the DPPH Scavenging assay. The results were compared to the standard antidiabetic drug, acarbose. Out of the 11 plants, five species demonstrated significant antidiabetic properties, inhibiting more than 50% of α-glucosidase and α-amylase enzyme activities. The petroleum ether tuber extract of Stephania glandulifera exhibited the highest α-glucosidase inhibition (99%), followed by the methanol leaf extract of Begonia roxburghii (94%) and the water leaf extract of Antidesma acidum (85%). In the α-amylase inhibition assay, Equisetum debile methanol extracts showed a 64% inhibition, followed by the leaf extract of A. acidum with a 62% inhibition in the methanol leaf extracts. Comparatively, methanol and water leaf extracts of Debregeasia longifolia displayed 64% and 67% inhibitions, respectively. The water extract of B. roxburghii demonstrated significant antioxidant activity (57%), while the other samples showed less than 50% effectiveness. Notably, the α-glucosidase inhibition consistently exceeded the α-amylase inhibition across the samples. This study confirms the antidiabetic properties of five out of the 11 ethnomedicinal plants, as evidenced by the in-vitro α-glucosidase and α-amylase inhibitory assays. These findings support the traditional use of these plants in the management of diabetes and emphasize the need for further in-vivo studies, which could contribute to the development of novel herbal formulations or therapeutic drugs for the treatment of diabetes.
Asian Journal of Pharmaceutical and Clinical Research
Objective: Diabetes mellitus is a highly prevalent chronic disease in Egypt leading to high socioeconomic problems, especially in the cities due to the unhealthy life style. Although many drugs are available, they have many side effects. Furthermore, the body arouses resistance after a while for the drug so it should be changed every once in a while. Plants could be a good source for drugs. In Egypt, we have a rich flora which has not been subjected to systematic screening for antidiabetic activity.Methods: The aim of this work was to screen 264 plant extracts for their in vitro α-glucosidase inhibitory activity. Those extracts which gave more than 70% inhibition were screened on different concentrations and their inhibitory concentrations giving 50% activity (IC50) were calculated.Results: Out of all the tested extracts, 63 gave more than or equal 70% inhibition on α-glucosidase at the tested concentration (25 ppm). After the calculation of the IC50 values, 10 extracts were chosen ...