Serum level of eosinophilic cationic protein in relation to eosinophilia and to exhaled nitric oxide value in children with atopic asthma and aeroallergens sensitization (original) (raw)
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Clinical <html_ent glyph="@amp;" ascii="&"/> Experimental Allergy, 1994
Children less than 5 yearsofage wilh aslhma wereasscsscd for total eosinophil counlsand scrum levels of the eosinophil proteins, eosinophil cationic protein (ECP) und eosinophil protein X (EPX). to determine whether these meastirements would rellect eosinophilic inllammation in the airways. Initially 27 symptomatic patients. 14 atopic and 13 nonatopic were investigated. They had a mean age of I -8 years and had never been treated with Inhaled steroid and had not received Intal for 2 weeks prior to the assessment. The 14 atopic patients proved to have higher mean total eosinophil counts and serum levels o{ ECP and EPX than the 13 non-atopic patients (eosinophil eounts 0-63x10^1 vs 0 26 X IO''/I. P < OOOl: ECP .369 figll vs 10-8 ^ig/1. /' < 0001: EPX 690 /ig/1 r,v 19 6 /ig/l. /'<0 01 ).Thirteen of thesepatientsrequired treatment with dailydosesof inhaled steroid and 11 had a repeat assessment (seven atopie and four non-atopic). The mean serum ECP ofthe seven atopic patients had fallen signiticantly (40 6 to 22-9. P < 0 05) while the total eosinophil counts did not. These results suggest a dillerence in numbers and activity of eosinophils in atopie compared with non-atopic asthma in young children.
Serum Eosinophilic Cationic Protein (ECP) in Asthmatic Malaysian Children
The Medical journal of Malaysia, 2002
Eosinophilic inflammation in the airways is important in the pathogenesis of childhood asthma. Serum eosinophilic cationic protein (ECP), a marker of eosinophil activation was measured in 20 asthmatic children and 19 non-asthmatic controls. There was no difference in the socio-demography, passive smoke exposure, urinary cotinine levels and family history of asthma between the 2 groups. The median serum ECP in asthmatic children was 27.0 mcg/L (IQ1 8.8, IQ3 59.0); which was higher than in non-asthmatic controls [5.9 mcg/L (IQ1 3.0, IQ3 11.9), p=0.002]. An elevated serum ECP level can be helpful as supportive evidence in the diagnosis of bronchial asthma in Malaysia children.
Allergy, 2001
Background: Most previous studies on eosinophil cationic protein (ECP) have been performed on carefully selected groups of asthmatic patients. Few studies based upon population cohorts have been reported. The primary objective of the present study was to assess the usefulness of serum eosinophil cationic protein (s-ECP) in the diagnosis of asthma in schoolchildren and determine reference values based on measurements in healthy children. Methods: The population consisted of 216 schoolchildren (aged 7±16 years) who in a previous questionnaire had reported asthma or asthma-like symptoms and a control group. The questionnaire study comprised the entire population of schoolchildren in Upper Hallingdal. After clinical assessment, blood samples, and skin prick tests, these subjects were then reclassi®ed into four groups: atopic and nonatopic asthmatic and nonasthmatics. S-ECP was assessed in relation to atopy, asthma severity, allergen exposure, and sex. Results: The asthma group (n=105) had signi®cantly higher mean s-ECP level than the nonasthma group (n=111) (13.3 vs 8.3 mg/l, P<0.001), with no signi®cant difference between atopic asthmatics and atopic nonasthmatics. Mean s-ECP levels in children with mild, moderate, or severe asthma were 12.1, 18.5, and 12.2 mg/l, respectively. The children with animal dander allergy demonstrated higher levels of s-ECP than children without this allergy (12.9 vs 9.1 mg/l, P=0.001). The upper reference limit (determined as the 95th percentile in healthy children) of 19.1 mg/l, showed low sensitivity (24%) and high speci®city (93%) for the diagnosis of asthma. The positive and negative likelihood ratios for the asthma diagnosis were found to be 3.2 and 0.83, respectively. Conclusions: The highest s-ECP values were found among children with moderate asthma. Animal dander allergy resulted in elevated s-ECP. However, mean values were still below the reference value of 19.1 mg/l, and the sensitivity was low, suggesting that s-ECP is not a useful parameter for diagnosing asthma in population-based studies.
Eosinophilic cationic protein: Is it useful in assessing control of childhood asthma?
Eastern Mediterranean health journal = La revue de santé de la Méditerranée orientale = al-Majallah al-ṣiḥḥīyah li-sharq al-mutawassiṭ
This study evaluated peripheral eosinophil and serum eosinophilic cationic protein (s-ECP) levels as markers of asthma control. A total of 38 children with asthma (16 controlled and 22 partially controlled) were compared with 16 age- and sex-matched healthy children. Total asthma cases had higher eosinophil counts and s-ECP levels than healthy children and partially controlled asthmatics had significantly higher levels of both markers than controlled asthmatics. Controlled asthma cases showed non-significant changes in both parameters versus healthy children. A negative correlation was noted between degree of asthma control and both eosinophil counts and s-ECP levels (r = -0.60 and -0.75 respectively). s-ECP as well as peripheral eosinophil count may be helpful in the assessment of asthma control.
Serum eosinopholic cationic protein (ECP) in asthmatic Malaysian children
Med J Malaysia, 2002
Eosinopholic inflammation in the airways is important in the pathogenesis of childhood asthma. Serum eosinopholic cationic protein (ESP), a marker of eosinophil activation was measured in 20 asthmatic smoke esposure, urinary cotinine levels and family history of asthma between the two groups. The median serum ECP in asthmatic children was 27.0 mcg/L (IQ1 8.8, IQ3 59.0); which was higher than in non-asthmatic controls [5.9 mcg/L (IQ1 3.0, IQ3 11.9)], p=0.0021. An elevated serum ECP level can be helpful as supportive evidence in the diagnosis of bronchial asthma in Malaysia children
Clinical <html_ent glyph="@amp;" ascii="&"/> Experimental Allergy, 1993
Blood eosinophils. and serutn levels of the eosinophil proteins, eosinophil cationic protein (ECP) and eosinophil protein X (EPX) were measured in childhood aslhma. Seventeen patients mean age 119 years who were symptomatic with asthma, were enrolled in a study examining the eosinophil counts and eosinophil proteins at the onset of study and after treatment in relation to changes in their baseline forced expiratory volume at 1 second (FEVi) and 'yupredicted FEV|. The patients with symptomatic asthma were compared with 17 patients mean age 12-0 years with asymptomatic asthma maintained on daily inhaled steroid and 13 patients, mean age 120 years, without asthma but with urticaria who served as non-asthma controls. Patients with symptomatic asthma did not have significantly higher initial eosinophil counts compared with those with asymptomatic asthma (0 43 x 10"/! vs 0 26 x lO'M. P = 009) but had higher serum ECP levels (28 9 fig/l vs 18 5 /.tg/1). Both asthma patient groups had signiticantly higher serum ECP levels (7'<00I) than the controls (9 8 /ig/l). After therapy consisting of increased dose of inhaled steroids and/or oral steroids, patients in the symptomatic asthma group demonstrated a significant rise in FEV| (I -67 I/sec at Visit I vs 208 !/secat Visit 2. /*<OOOI). A similar rise was seen for 'Mipredicted FEV|. Patients in the asymptomatic asthma group showed no significant change in FEV| between visits (2 23 I/sec vs 2-37 l/scc). which was verified with the "(.predicted FEVi. Patients in the symptomatic asthma group showed a significant decrease in ECP level following treatment (28 9 /jg/l to 9-6 /(g/1. P<0 001) while the values in the asymptomatic group did not change (18-6/ig/l to 15 2 //g/1 not significant). There was a significant correlation between the initial ECP level in the symptomatic asthma group and the change in the FEVi with treatment. Serum EPX levels showed similar trends btit there was no significant correlation between the initial EPX levels and the changes in FEV|. Neither did blood eosinophil counts show such a correlation. This data suggests that the changes in scrum ECP levels correlate with the changes in Umg function subsequently to antiinflammation therapy.
Serum eosinophil cationic protein in children with atopic dermatitis
International Journal of Dermatology, 2006
Background: Eosinophil cationic protein (ECP) is a cytotoxic agent secreted by activated eosinophils during allergic and inflammatory processes. The aim of the study was to determine ECP level, absolute and relative eosinophil count and IgE antibodies in children with atopic dermatitis (AD), compare them to the levels in nonatopic children, and to assess correlation of these laboratory parameters with clinical severity of AD. Methods: The prospective study encompassed 70 children. There were 49 children with AD aged 3 to 36 months and the control group comprised 21 children, with negative personal and family history for atopic diseases. Detailed history, serum ECP levels (UniCAP FEIA), relative and absolute eosinophil counts and total serum IgE antibodies were determined in both groups. In AD children the skin involvement was measured by SCORAD index. Results: The calculated SCORAD index was between 16 and 83. IgE antibodies, relative and absolute eosinophil counts showed a much wider range of values and a statistically higher median (p<0.001) in AD patients compared to the control group. These laboratory parameters did not correlate with the severity of AD. The serum ECP median level, in children with AD, was 16.2 µg/L (range 3.01-65.30) compared to 5.92 µg/L (range 2.76-21.90) in the control group. The correlation of total SCORAD index and serum ECP levels was negative, weak (r=-0.065) and statistically not significant (p>0.05). The same was found for the correlation of serum ECP and intensity of skin changes (r=-0.095) and serum ECP and subjective symptoms (r=-0.045). The correlation was positive, but weak and statistically not significant for serum ECP and extent of skin lesions (r=0.079, p>0.05). Conclusion: Elevated levels of ECP, relative and absolute eosinophil counts, as well as IgE antibodies were determined in the patients with AD.
Urinary Eosinophil Protein X in Children with Atopic Asthma
Mediators of Inflammation, 2007
The aim of this study was to investigate the relationship between urinary eosinophil protein X (uEPX) and asthma symptoms, lung function, and other markers of eosinophilic airway inflammation in asthmatic school children. Methods. A cross-sectional study was performed in 180 steroid dependent atopic children with stable moderately severe asthma, who were stable on 200 or 500μg of fluticasone per day. uEPX was measured in a single sample of urine and was normalized for creatinine concentration (uEPX/c). Symptom scores were kept on a diary card. FEV 1 and PD 20 methacholine were measured. Sputum induction was performed in 49 and FE NO levels measured in 24 children. Results. We found an inverse correlation between uEPX/c and FEV 1 (r = −.20, P = .01) and a borderline significant correlation between uEPX/c and PD 20 methacholine (r = −.15, P = .06). Symptom score, %eosinophils and ECP in induced sputum and FE NO levels did not correlate with uEPX/c. Conclusion. uEPX/c levels did not correlate with established markers of asthma severity and eosinophilic airway inflammation in atopic asthmatic children.