Composite Hemostatic Nonwoven Textiles Based on Hyaluronic Acid, Cellulose, and Etamsylate (original) (raw)
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The design and testing of a dual fiber textile matrix for accelerating surface hemostasis
Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2009
The standard treatment for severe traumatic injury is frequently compression and application of gauze dressing to the site of hemorrhage. However, while able to rapidly absorb pools of shed blood, gauze fails to provide strong surface (topical) hemostasis. The result can be excess hemorrhage-related morbidity and mortality. We hypothesized that cost-effective materials (based on widespread availability of bulk fibers for other commercial uses) could be designed based on fundamental hemostatic principles to partially emulate the wicking properties of gauze while concurrently stimulating superior hemostasis. A panel of readily available textile fibers was screened for the ability to activate platelets and the intrinsic coagulation cascade in vitro. Type E continuous filament glass and a specialty rayon fiber were identified from the material panel as accelerators of hemostatic reactions and were custom woven to produce a dual fiber textile bandage. The glass component strongly activated platelets while the specialty rayon agglutinated red blood cells. In comparison with gauze in vitro, the dual fiber textile significantly enhanced the rate of thrombin generation, clot generation as measured by thromboelastography, adhesive protein adsorption and cellular attachment and activation. These results indicate that hemostatic textiles can be designed that mimic gauze in form but surpass gauze in ability to accelerate hemostatic reactions.
Hemostatic, Resorbable Dressing of Natural Polymers-Hemoguard
Autex Research Journal, 2016
Investigations are presented for the preparation of a model hemostatic dressing that would exhibit an adequate hemostatic capacity in injuries and surgical wounds, an antibacterial activity to prevent primary and secondary infections, and offer safety in use. The Hemoguard dressing has been designed as a powder prepared from the complex chitosan/alginate Na/Ca in the form of micro- and nano-fibrids. Useful antibacterial and hemostatic properties of Hemoguard, which would qualify the material as first aid dressing and a temporary protection of injury wounds in field conditions, were assessed. Biocompatibility of the dressing was confirmed by biological in vitro examinations.
Bakirkoy Tip Dergisi / Medical Journal of Bakirkoy, 2021
Objective: This study aimed to analyze the effect of an absorbable hemostat produced from oxidized regenerated cellulose (ORC) on pelvic adhesion formation in a rat model using an adhesion scoring system and immunohistochemical staining. Methods: This randomized, controlled experimental study included 20 female Wistar-Albino rats that were equally divided into the following groups: control and absorbable hemostat groups. The uterine horns of all the rats were exposed by laparotomy and using 10 W bipolar cautery. Five standard lesions were applied to the antimesenteric areas of each uterine horn. The experimental group received an absorbable hemostat to the traumatized uterine surfaces, whereas the control group did not. After a 28-day follow-up period, a relaparotomy was performed, and adhesions were evaluated based on an adhesion scoring system, and histological sections from areas with adhesion were obtained for immunohistochemical staining. Immunohistochemical staining included analysis of Ki-67 (proliferation index), CD-31 (neovascularization index), and Masson Trichrome [(MTC) fibrosis and collagen formation index]. Additionally, acute and chronic inflammation indices were determined via polymorphonuclear leukocytes (PMNL) and mononuclear leukocytes (MNL), respectively. Results: The intensity and scope of adhesion and overall adhesion ratings were substantially higher in the absorbable hemostat group than the control group (2.8±0.85 vs. 2.2±0.53, 0.92±0.26 vs. 0.61±0.25, and 3.72±0.96 vs. 2.81±0.75, respectively). Staining results for Ki-67, CD-31, MTC, PMNL, and MNL were also significantly higher in the absorbable hemostat group than in the control group (p<0.05 for all). Conclusion: The obtained results suggest that the use of ORC-based absorbable hemostats in pelvic surgery may increase adhesion formation on peritoneal surfaces by increasing inflammation, vascularity, and collagen formation.
Properties of collagen-based hemostatic patch compared to oxidized cellulose-based patch
Journal of materials science. Materials in medicine, 2018
Two self-adhering hemostatic patches, based on either PEG-coated collagen (PCC) or PEG-coated oxidized cellulose (PCOC), are compared regarding to maximum burst pressure, mechanical stability, and swelling. In addition, the induction of tissue adhesions by the materials was assessed in a rabbit liver abrasion model. Both materials showed comparable sealing efficacy in a burst pressure test (37 ± 16 vs. 35 ± 8 mmHg, P = 0.730). After incubation in human plasma, PCC retained its mechanical properties over the test period of 8 h, while PCOC showed faster degradation after the 2 h time-point. The degradation led to a significantly decreased force at break (minimum force at break 0.55 N during 8 h for PCC, 0.27 N for PCOC; p < 0.001). Further, PCC allowed significantly higher deformation before break (52% after 4 h and 50% after 8 h for PCC, 18% after 4 h and 23% after 8 h for PCOC; p = 0.003 and p < 0.001 for 4 h and 8 h, respectively) and showed less swelling in human plasma (max...
Journal of Endourology, 2007
The aim of this prospective study was to evaluate the advantages or disadvantages of the use of fibrin glue and collagen fleece during laparoscopic partial nephrectomy. Patients and Methods: Two groups of patients were studied. Group A (n ؍ 24) received parenchymal suture, whereas Group B (n ؍ 20) received parenchymal suture with fibrin glue and collagen fleece. The two groups were similar in baseline characteristics. We evaluated patient age, size of the lesion at CT, operative time, ischemia time, and sealant technique in relation to blood loss, hospital stay, and hemorrhagic complications. Results: No significant difference was observed in perioperative parameters (P Ͼ 0.05). The mean size of lesion was 3.3 ؎ 1.2 (range 1-8 cm) for group A and 3.0 ؎ 1.3 (range 2-5 cm) for Group B. The mean operative time was 116 ؎ 26.6 minutes (range 90-220 minutes) for group A and 130 ؎ 23.5 minutes (range 90-210 minutes) for group B. The mean warm ischemia time was 28.8 ؎ 5.7 minutes (range 18-60) minutes) and 35.6 ؎ 6.2 minutes (range 20-52 minutes), respectively. The mean blood loss was 178 ؎ 34.5 mL (range 50-400 ml) for group A and 219 ؎ 44.6 mL (range 80-750 ml) for group B. The mean hospital stay was 5.9 ؎ 1.2 days (range 5-8 days) for group A and 6.3 ؎ 2.1 days (range 5-9 days) for group B. Four and two postoperative hemorrhage complications were observed in groups A and B, respectively. Conclusion: The use of fibrin glues and collagen fleece should be considered an adjuvant, as it does not present any substantial advantages, the suture being the key point in hemostasis control. We believe that in order to improve hemostasis, the efficacy of other types of sealants should be studied, as we were not convinced by those we used.
Silk Fibroin-Based Biomaterials for Hemostatic Applications
Biomolecules
Hemostasis plays an essential role in all surgical procedures. Uncontrolled hemorrhage is the primary cause of death during surgeries, and effective blood loss control can significantly reduce mortality. For modern surgeons to select the right agent at the right time, they must understand the mechanisms of action, the effectiveness, and the possible adverse effects of each agent. Over the past decade, various hemostatic agents have grown intensely. These agents vary from absorbable topical hemostats, including collagen, gelatins, microfibrillar, and regenerated oxidized cellulose, to biologically active topical hemostats such as thrombin, biological adhesives, and other combined agents. Commercially available products have since expanded to include topical hemostats, surgical sealants, and adhesives. Silk is a natural protein consisting of fibroin and sericin. Silk fibroin (SF), derived from silkworm Bombyx mori, is a fibrous protein that has been used mostly in fashion textiles and...
Recent advances in biopolymer-based hemostatic materials
Regenerative Biomaterials
Hemorrhage is the leading cause of trauma-related deaths, in hospital and prehospital settings. Hemostasis is a complex mechanism that involves a cascade of clotting factors and proteins that result in the formation of a strong clot. In certain surgical and emergency situations, hemostatic agents are needed to achieve faster blood coagulation to prevent the patient from experiencing a severe hemorrhagic shock. Therefore, it is critical to consider appropriate materials and designs for hemostatic agents. Many materials have been fabricated as hemostatic agents, including synthetic and naturally derived polymers. Compared to synthetic polymers, natural polymers or biopolymers, which include polysaccharides and polypeptides, have greater biocompatibility, biodegradability and processibility. Thus, in this review, we focus on biopolymer-based hemostatic agents of different forms, such as powder, particles, sponges and hydrogels. Finally, we discuss biopolymer-based hemostatic materials ...
International braz j urol : official journal of the Brazilian Society of Urology
To analyze the interaction between the cellulose exopolysaccharide (CEC) and urethral tissue when used as a pubovaginal sling. Forty Wistar rats were divided into four groups. In groups A and B the cellulose exopolysaccharide (CEC) was implanted around the urethral tissue (bladder neck below the upper margin) and the rats were sacrificed at 30 and 90 days. Similar procedure was used in groups C and D using a polypropylene mesh. After sacrifice bladder and urethra were sent for histological analysis. The histological parameters (inflammatory reaction) by evaluated by quantitative analysis. For collagen deposition analysis it was used stereological method. The cellulose exopolysaccharide (CEC) was inert and well preserved at the implanted region at the time of examination. Morphologic alterations were not found at the CEC implant but some reactions of foreign body type were observed at the adjacent structures. In some areas a process of neovascular formation was observed. Stereologica...
RECENT DEVELOPMENTS IN MEDICAL TEXTILES
An important field of application of textile in medicine has been developed such as wound care and preventing chronic wounds. Bandages and wound dressings are most commonly used because they are affordable and reusable. The medical textile should have bio-compatibility, flexibility and strength. In surgical dressings the “sorbagon” dressings are innovated to produce more comfort in the dressing of wounds. Some of the super absorbent polymers are innovated, which includes polyacrylate and sodium acrylate are made to improve the absorbency of the fibres. The recently developed calcium alginate. Fibres have high super absorbing capacity. Recent advances and specific requirements necessitated innovation in surgical sutures which includes barbed, gelatin coated catgut sutures. A recent development of silk fibre includes “spider silk” which has been obtained from goat milk by injecting the genes of spider into mammary glands of goat. The textile materials have generated considerable interest in medical technology where materials in the form of monofilament, multifilament, woven and nonwovens structures are being used for bio and medical applications. The major requirement of the textile materials is the bioreceptivity and biocompatibility at the application site in human being.