Enhancing Antioxidant Properties of Prunus spinosa Fruit Extracts via Extraction Optimization (original) (raw)
Related papers
Antioxidants
This study aimed at establishing the optimal conditions for the classic extraction of phenolic compounds from Prunus spinosa L. fruits. The effects of different parameters, i.e., ethanol concentration in the extraction solvent (mixture of ethanol and water), operation temperature, and extraction time, on process responses were evaluated. Total phenolic content (TPC), total anthocyanin content (TAC), antioxidant capacity (AC), and contents of protocatechuic acid (PA), caffeic acid (CA), vanillic acid (VA), rutin hydrate (RH), and quercetin (Q) of fruit extracts were selected as process responses. A synergistic effect of obtaining high values of TPC, TAC, AC, PA, and VA was achieved for the extraction in 50% ethanol at 60 °C for 30 min. At a higher level of process temperature, the extraction of protocatechuic acid and vanillic acid was enhanced, but the flavonoids, i.e., rutin hydrate and quercetin, were degraded. A lower temperature should be used to obtain a higher amount of flavon...
Phytochemical characteristics and antioxidant capacity of fruit extracts of different prunus species
2016
Fruits are one of the major sources of polyphenol compounds in human diet. These compounds are known to have many health-promoting activities, especially anticancer, antiradical and antioxidant effects. In this work, seven different Prunus species traditionally grown in south Bačka region of Vojvodina were investigated: blackthorn (P. spinosa), plum (P. domestica), apricot (P. armeniaca), cherry plum (P. cerasifera), sweet cherry (P. avium), sour cherry (P. cerasus) and mahaleb cherry (P. mahaleb). Freeze-dried fruits were evaluated in terms of their phytochemical characteristics and bioactivity, determining total content of phenolics, flavonoids and anthocyanins, total antioxidant capacity and antiproliferative effect on human colon cancer cells (HT29). Blackthorn fruits are the richest in phenolic and flavonoid contents, while mahaleb cherry and sweet cherry had much higher content of total anthocyanins than other examined species. Apricot and cherry plum fruits had the lowest lev...
EVALUATION OF THE ANTIOXIDANT ACTIVITIES OF PRUNUS DOMESTICA WHOLE FRUIT: AN IN VITRO STUDY
Objective: Prunus domestica is known for its nutritional values and therapeutic properties. It contains rich amount of natural phenolic phytochemicals such as phenolic acids, flavonoids and anthocyanin. Previous reports suggest that prune extract and juice inhibit peroxidation process. As no detailed antioxidant property, particularly on the whole fruit was studied, in this investigation, we not only evaluated the detailed antioxidantive activities of different fractions of P. domestica fruit but also tried to correlate the same with total phenol and flavonoids contents. Methods: Simple warring blender method for extraction, folin- ciocalteu method for total phenolic content, aluminium chloride colorimetric method for total flavonoid content were employed. Different antioxidant activities such as total antioxidant, iron chelating, hydrogen peroxide scavenging of superoxide radical and reducing power method were performed. Result: Simple warring blender method, used in the present study for the preparation of whole fruit extract exhibited better yield potential, total phenolic and flavonoid contents. A comparative study of different fraction types indicated that ethyl acetate fraction had very high phenolic and flavonoid contents that also exhibited maximum antioxidant activity. Butanol fraction also showed somewhat similar results. Conclusion: Ethyl acetate and n-butanol fractions showed maximum antioxidative potential. Further studies will be helpful to explore its therapeutic potential in treating different chronic diseases.
Hemijska industrija, 2014
Blackthorn (Prunus spinosa L.) is commonly used in food industry and phytotherapy. The contents of phenols, flavonoids, anthocyanins and antioxidative activity in extracts of blackthorn fruit were determined using spectrophotometric methods. The content of total phenol compounds varies from 15.33 to 20.94 mg GAE g-1 of fresh fruit. The content of total flavonoids is very low, and ranges from 0.419 to 1.31 mg QE g-1 of fresh fruits. Anthocyanins content lies between 0.112 mg cyanidin 3-glucoside/g of fresh sample in ethanol extract and 0.265 mg of cyanidin 3-glucoside g-1 of fresh blackthorn fruit in methanol-water 50/50 (v/v) extract. The differences in total phenol compounds content depend on used extraction medium as a consequence of different polarity of used organic solvents and their mixtures, which selectively extract individual compounds. All explored extracts exhibited strong scavenging activity against DPPH radicals, which ranges from 32.05 to 89.10%. Phenolic acids (neochl...
International Journal of Horticultural Science, 2011
Fruits of nine Prunus spinosa and P. insititia selections were compared in their ferric reducing antioxidant power and total phenoliccontent. The antioxidant capacity and total phenolic content ranged between 6.36 and 29.26 mmol AA/L, and 5.04 and 29.71 mmol GA/L,respectively. These ranges cover an almost 5-times variation among the tested genotypes. The Pearson’s coefficient was very high (0.92)indicating a major contribution of polyphenolics to the antioxidant capacity of the tested Prunus fruits. Conserving resulted in an approximate20 % loss of antioxidant power and slightly increased phenolic contents. Our results led us to the conclusion that fruit of Prunus spinosa andP. insititia might be considered as rich sources of antioxidants. In addition, procession with heat treatment caused only a slight decrease in theantioxidant capacity without loss in the total polyphenolic content.
Prunus spinosa fresh fruit juice: antioxidant activity in cell-free and cellular systems
Natural product communications, 2009
The antioxidant activity was assessed of fresh juice from Prunus spinosa L. fruit (Rosaceae) growing wild in Urbino (central Italy) by using different cell-free in vitro analytical methods: 5-lipoxygenase test, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, and oxygen radical absorbance capacity (ORAC). Trolox was used as the reference antioxidant compound. In the 5-lipoxygenase and DPPH tests the fresh fruit juice of P. spinosa showed good antioxidant activity when compared with Trolox, while the ORAC value was 36.0 micromol eq. Trolox/g of fruit. These values are in accord with data reported in the literature for small fruits such as Vaccinium, Rubus and Ribes. The antioxidant capacity in cell-free systems of P. spinosa juice has been compared with its cytoprotective - bona fide antioxidant activity in cultured human promonocytes (U937 cells) exposed to hydrogen peroxide. The antioxidant activity of red berries has been correlated with their anthocyanin content. The...