Chamoli, M., Pandit, A., Sudharsan, M., & Mukhopadhyay, A. (2013). Novel Kinases that interact with the Insulin-IGF-1-like signaling pathway to control longevity presented in International Worm Meeting. Unpublished information; cite only with author permission (original) (raw)

Abstract

Extensive research over the past decade has identified the Insulin-IGF-1-like signaling (IIS) axis, predominantly a kinase cascade, as a major regulator of aging across the animal kingdom. In C. elegans, the IIS pathway regulates dauer diapause, metabolism, stress\/pathogen resistance as well as longevity. In order to identify additional kinases that may interact or cross-talk with this pathway and regulate life span, we developed a high throughput 96-well liquid RNAi screening protocol using dauer formation as the quantifiable output. We prepared a kinase mini-library that contained RNAi clones for around 400 serine-threonine kinases and screened for ones that affect dauer formation in daf-2(e1370). We identified several novel as well as previously studied modulators of the IIS pathway. Here we report characterization of two such kinases that enhanced dauer formation of an IIS pathway mutant. Although both the kinases possess activity in vitro and increased dauer formation of daf-2...

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