Patterns of Chromosomal Evolution in Sigmodon, Evidence from Whole Chromosome Paints (original) (raw)
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Chromosome Research, 2009
Traditionally comparative cytogenetic studies are based mainly on banding patterns. Nevertheless, when dealing with species with highly rearranged genomes, as in Akodon species, or with other highly divergent species, cytogenetic comparisons of banding patterns prove inadequate. Hence, comparative chromosome painting has become the method of choice for genome comparisons at the cytogenetic level since it allows complete chromosome probes of a species to be hybridized in situ onto chromosomes of other species, detecting homologous genomic regions between them. In the present study, we have explored the highly rearranged complements of the Akodon species using reciprocal chromosome painting through speciesspecific chromosome probes obtained by chromosome sorting. The results revealed complete homology among the complements of Akodon sp. n. (ASP), 2n=10; Akodon cursor (ACU), 2n=15; Akodon montensis (AMO), 2n=24; and Akodon paranaensis (APA), 2n=44, and extensive chromosome rearrangements have been detected within the species with high precision. Robertsonian and tandem rearrangements, pericentric inversions and/or centromere repositioning, paracentric inversion, translocations, insertions, and breakpoints, where chromosomal rearrangements, seen to be favorable, were observed. Chromosome painting using the APA set of 21 autosomes plus X and Y revealed eight syntenic segments that are shared with A. montensis, A. cursor, and ASP, and one syntenic segment shared by A. montensis and A. cursor plus five exclusive chromosome associations for A. cursor and six for ASP chromosome X, except for the heterochromatin region of ASP X, and even chromosome Y shared complete homology among the species. These data indicate that all those closely related species have experienced a recent extensive process of autosomal rearrangement in which, except for ASP, there is still complete conservation of sex chromosomes homologies.
Chromosome Research, 2012
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Comparative Cytogenetics, 2016
The genus Akodon Meyen, 1833 is one of the most species-rich among sigmodontine rodents and has great chromosome variability. Akodon montensis has a relatively broad distribution in South America, and Argentine populations are located in the southernmost region of its range. Brazilian populations have important chromosomal variability, but cytogenetic data from Argentina are scarce. We performed a chromosome characterization of natural populations of A. montensis using conventional staining, C-banding, Ag-NORs and base-specific fluorochromes. A total of 31 specimens from five localities of Misiones Province, in Argentina, were analyzed. The 2n=24 chromosomes was the most frequently observed karyotype. However, five individuals presented 25 chromosomes due to a supernumerary B-chromosome; and one individual had 2n=26 due to one B plus a trisomy for chromosome 11. Additionally, two XY females and two variants of the X chromosomes were found. C-positive centromeric bands occurred in all chromosomes; additional C-bands were observed in some autosomes, the X, Y and B chromosomes. Ag-NORs were observed in five autosomes, and the B chromosome was frequently marked. Fluorochrome banding was similar among karyotypes of the analyzed populations. Comparisons of cytogenetic data among populations of Argentina and Brazil showed the presence of high intraspecific variability in A. montensis and some differences among regions.
Hereditas, 2004
All available published cytogenetic data show the presence of 28 different karyotypes in 31 1 specimens of A. cur.vor as an exceptional example of high karyotype variability in a single species. Our present sample of 116 animals collected in the rain forest of the Atlantic coast of the states of Sgo Paulo and Bahia, Brazil, show 25 karyotype constitutions. The diploid number (2n) ranged from 16 to 14, and the number of autosomal arms (NF) from 26 to 18, because of centric fusion and pericentric inversions involving two autosome pairs, pericentric inversions in three other chromosome pairs, trisomy in the pair 7 and the presence of two XO females. Synaptonemal complex analysis, associated with data from experimental cross-breeding, suggested that heterozygous individuals for pericentric inversions have normal fertility. In this paper, we have reviewed the chromosomal data of this species, and have thus standardized the karyotype description and chromosome numbering. We discuss about karyotype evolution of Akodon cursor based on the frequency and constitution of karyotypes of all different geographical samples described so far in the literature.
Anais Da Academia Brasileira De Ciencias, 2022
Rodentia has a high species number and chromosomal variability. The South American genus Akodon is one of the most speciose muroids, with more than 40 species included in several species groups. Here, we characterize cytogenetically specimens of Akodon from central-western Argentina. Subsequently, we reviewed and analyzed the cytogenetic data for this genus, build a phylogeny and mapped chromosome changes to interpret its evolution. Specimens of A. dolores from central-western Argentina have 2n=42-44/FNa=44 (46, 48) due to a Robertsonian rearrangement. Our data expand the distribution range known for this polymorphism and confirm its geographic structure. Other specimens had 2n=40/FNa=40, representing populations of A. oenos, A. polopi, and A. spegazzinii. All karyotypes have a low amount of heterochromatin, concentrated in centromeres and sex chromosomes, as in other rodents. The complement with 2n=40/ FNa=40 is the most frequent in Akodon and is shared by most species in some groups. Chromosome numbers are very diverse. The FNa shows less variability; FNa=42 was recovered as ancestral, excluding A. mimus, which was connected at the base of the Akodon tree and has FNa=44. This indicates a complex chromosome evolution in Akodon, and suggests that reductions and increases in the 2n and FNa evolved independently in some lineages.
International Journal of Molecular Sciences
Comparative chromosome-painting analysis among highly rearranged karyotypes of Sigmodontinae rodents (Rodentia, Cricetidae) detects conserved syntenic blocks, which are proposed as chromosomal signatures and can be used as phylogenetic markers. In the Akodontini tribe, the molecular topology (Cytb and/or IRBP) shows five low-supported clades (divisions: “Akodon”, “Bibimys”, “Blarinomys”, “Oxymycterus”, and “Scapteromys”) within two high-supported major clades (clade A: “Akodon”, “Bibimys”, and “Oxymycterus”; clade B: “Blarinomys” and “Scapteromys”). Here, we examine the chromosomal signatures of the Akodontini tribe by using Hylaeamys megacephalus (HME) probes to study the karyotypes of Oxymycterus amazonicus (2n = 54, FN = 64) and Blarinomys breviceps (2n = 28, FN = 50), and compare these data with those from other taxa investigated using the same set of probes. We strategically employ the chromosomal signatures to elucidate phylogenetic relationships among the Akodontini. When we ...
Cytogenetic and Genome Research, 2011
Chromosome painting with Akodon paranaensis chromosome 21 (a small metacentric akodont marker) paint revealed total homology with the smallest acrocentric Deltamys sp. chromosome, DSP19. This suggests the occurrence of a pericentric inversion or centromeric shift when compared to other akodontines, with a posterior tandem rearrangement giving rise to DKE4. In DKE, large blocks of pericentromeric constitutive heterochromatin are present on the autosomes and the X, and the Y/autosome has an entirely heterochromatic short arm. In DSP, small heterochromatic blocks are observed on autosomes and X, and the Y is a very small, mostly heterochromatic acrocentric. The cytogenetic analyses suggest that the Deltamys sp. karyotype is ancestral, with the derived condition resulting from a tandem fusion (DSP7 + DSP19) and the Y/autosome translocation giving rise to the multiple sex chromosome system. The autosomal rearrangements, the differences in CBG-banding patterns and Ag-NOR localization, as well as the presence of X 1 X 1 X 2 X 2 /X 1 X 2 Y and XX/XY sex determination mechanisms, possibly acting as a reproductive barrier, and the phylogenetic position within the Deltamys genus, with high genetic divergence, call for a taxonomic review of the genus.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 1997
Akodon cursor is an exceptional example of high chromosomal variability, displaying diploid numbers from 14 to 16 and fundamental numbers (FN) from 18 to 26 as the result of a complex rearrangement in par 1 and pericentric inversions in three autosomal pairs. The difference in the diploid number is due to the presence of a large metacentric pair 1 in the 2n=14 karyotype, a large metacentric 1 and two different submetacentrics (1a and 1b) in 2n=15 and 1a and 1b submetacentric pairs in homozygosis in the 2n = 16 karyotype. Chromosomes 1a and 1b share homology with the short and long arms of the large metacentric 1 respectively. In this paper, evidence based on fluorescence in situ hybridization (FISH) with telomeric sequences and G-banding indicates that pericentric inversions and fusion of chromosomes 1a and 1b are the probable rearrangements giving rise to the large metacentric 1.