HPTLC quantification of triterpenoid compounds and evaluation of in-vitro potential of Trichodesma indicum L (original) (raw)
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PLANT SCIENCE TODAY, 2020
Natural antioxidant capacity of five important folk medicinal plants measured in vitro. Total phenolic content (TPC), flavonoid content (FC) and free radical scavenging capacity of ethanolic, methanolic and aqueous leaf extracts of Lippia alba (LA), Annona squamosa (AS), Hyptis suaveolens (HS), Commiphora wightii (CW) and Milletia pinnata (MP) was assessed using spectrophotometric method. Folin ciocalteu and aluminium chloride method employed to optimise TPC and FC. Free radical scavenging potentiality of leaf extracts was assessed using Ferrous ion chelation (FIC), 2, 2'-diphenyl-1-picrylhydrazyl (DPPH •) scavenging, Hydroxyl (OH •) radical scavenging (HRS) and Superoxide (O-2) radical scavenging (SRS) methods. Results revealed that the TPC (96.22±5.85 to 519.23±34.90 μg GAE/gm dry weight) were found significant in aqueous extracts from all the plants except AS (p<0.05). For FC (μg QCE/gm dry weight), ethanol was found optimum for LA (463.94±6.49), CW (289.99±2.70) and MP (347.47±4.50) whereas, aqueous was found more appropriate for rest two plants were found significant instead of ethanol and methanol (all p<0.05). The lowest IC50 (μg/ml) were recorded from A. squamosa (27.72±8.95), H. suaveolens (27.78±0.88), C. wightii (27.18±0.16) and M. pinnata (27.30±0.03). All plants have reflected a high antioxidant capacity; however, the highest antioxidant activity was reported from ethanolic extract of H. suaveolens followed by L. alba, A. squamosa, C. wightii and M. pinnata. Hence, these studies show that all folk medicinal plants contain potential antioxidant bioactive compounds.
Plant Science today, 2020
Natural antioxidant capacity of five important folk medicinal plants measured in vitro. Total phenolic content (TPC), flavonoid content (FC) and free radical scavenging capacity of ethanolic, methanolic and aqueous leaf extracts of Lippia alba (LA), Annona squamosa (AS), Hyptis suaveolens (HS), Commiphora wightii (CW) and Milletia pinnata (MP) was assessed using spectrophotometric method. Folin ciocalteu and aluminium chloride method employed to optimise TPC and FC. Free radical scavenging potentiality of leaf extracts was assessed using Ferrous ion chelation (FIC), 2, 2'-diphenyl-1-picrylhydrazyl (DPPH•) scavenging, Hydroxyl (OH•) radical scavenging (HRS) and Superoxide (O- 2) radical scavenging (SRS) methods. Results revealed that the TPC (96.22±5.85 to 519.23±34.90 ?g GAE/gm dry weight) were found significant in aqueous extracts from all the plants except AS (p<0.05). For FC (?g QCE/gm dry weight), ethanol was found optimum for LA (463.94±6.49), CW (289.99±2.70) and MP (34...
2014
Antioxidant compounds in food play an important role as a health-protecting factor. Scientific evidence suggests that antioxidant reduces risks for chronic diseases including cancer and heart disease in the body. Present research was carried out to find potential antioxidant activity and developing HPTLC phytochemical fingerprint of ten important Indian medicinal plant extracts and to relate it with their medicinal and disease curing ability. Plant extracts prepared in various polarity solvents like water, methanol, chloroform, petroleum ether and screened for antioxidant activity using assays, Total Phenol content estimation, DPPH radical scavenging activity and Ferrous ion chelating activity. Comparative study of extracts prepared in different polarity solvents, successfully demonstrated the nature and polarity of antioxidant phyto-chemicals present in the studied plants and thus the results can be utilized for the value addition for future reference. Over all extracts of Lawsonia...
Applied Sciences
Given the important role of antioxidants in the cellular degeneration process, as well as the increased interest in recent years related to the use of natural antioxidants in therapy, the present study aims to investigate the antioxidant activity of a new pharmaceutical product containing natural antioxidants extracted from plant raw materials. In a first step, the product conventionally named “CILTAG”, containing a mixture in equal proportions of 10% hydroalcoholic extractive solutions of dried plant raw materials from Medicaginis herba, Trifolii pratense flores, Ginkgo bilobae folium, Myrtilli fructus, and Cynosbati fructus, was obtained. In the second stage, the antioxidant activity of the hydroalcoholic extractive solutions included in the pharmaceutical product was tested by chemiluminescence and electrochemical methods and by the superoxide dismutase (SOD) method. The electrochemical determination of the antioxidant capacity of hydroalcoholic extractive solutions was based on ...
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Salviae and Plantago species are plants known as having antioxidant effects. The aim of the study was to compare in vitro the antioxidant capacity of these medicinal plants from two different areas of Romania (Bihor and Arad counties) in order to use it in the future in the field of pharmacology and medicine. An UV-Vis spectra fingerprint (250-800 nm) of ethanol extracts from Salviae and Plantago species was recorded in order to establish the maximum absorption, specifically bioactive compounds. The bioactive compounds from those two extracts, polyphenols types were determined by the Folin-Ciocalteu method, and the total flavonoids have been determined through a spectrophotometric method. Antioxidant capacity of the extracts was evaluated by the following methods: DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) and FRAP (the Ferric reducing antioxidant power). The results shown, that Salviae species are rich in polyphenols, while Plantago is rich in flavonoids being a good reason for their association.
Journal of Pharmaceutical Research International
Aim: The present work was aimed at evolving pharmacognostical and phytochemical quality standards for certain traditional herbs like Phyllanthus amarus, Glycerrhiza glabra and Piper nigrum. These three plants are reported to possess antidiabetic activity. Study Design: The plants were collected, authenticated and Macro-morphological, qualitative and quantitative microscopic features as well as physicochemical, fluorescence analysis, phytochemical properties, and thin layer chromatography (TLC) Place and Duration of Study: The study was carried out at College of Pharmacy, Dr. APJ Abdul Kalam University, Indore, during 2019-20 Methodology: The material were collected, authenticated and Macro-morphological, qualitative and quantitative microscopic features as well as physicochemical, fluorescence analysis, phytochemical properties, and thin layer chromatography (TLC) profile of Phyllanthus amarus, Glycerrhiza glabra and Piper nigrum were determined using standard methods. Results: The...
2022
Oxidative stress and free radicals-induced diseases can be managed by introducing clinically proven naturally occurring antioxidants or antioxidant supplements which can prevent the onset of many cardiovascular diseases, neurodegenerative disorders, and cancers. Collection, identification, and preservation of plant material, sample preparation from plant sources using suitable solvents. Rapid screening of plant extracts for antioxidant activity and Electron Transfer methods (ET) assay. In-vitro Antioxidant Methods Hydrogen Atom Transfer methods (HAT). These antioxidants have a strong potential in the conventional treatments of diseases especially in inflammatory disease, neurodegenerative diseases, cancer, and diabetes. Hence, with a balanced diet and best supplementation of fruits, vegetables, grains, oils, and nuts having adequate essential antioxidants such as vitamin A, E, C, lipoic acid, etc., are sufficient to improve our body’s immune system.
2008
Due to differences in the functional quality of natural extracts, we have also faced differences in their effectiveness. So, it was intended to assess the antioxidant activity of natural extracts in order to attain their functional quality. It was observed that all the extracts (brown and green propolis, Ginkgo biloba and Isoflavin Beta®) and the standard used (quercetin) showed antioxidant activity in a dose-dependent manner with IC 50 values ranging from 0.21 to 155.28 μg mL-1 (inhibition of lipid peroxidation and scavenging of the DPPH • assays). We observed a high correlation (r 2 = 0.9913) among the antioxidant methods; on the other hand, the antioxidant activity was not related to the polyphenol and flavonoid content. As the DPPH • assay is a fast method, presents low costs and even has a high correlation with other antioxidant methods, it could be applied as an additional parameter in the quality control of natural extracts. RESUMEN. "Evaluación de la actividad antioxidante como parámetro adicional para lograr la calidad funcional de extractos naturales". Debido a las diferencias en la calidad funcional de extractos naturales, nosotros hemos advertido también diferencias en su efectividad, por lo que se pretende estimar la actividad antioxidante de extractos naturales para lograr su calidad funcional. Fue observado que todos los extractos (propolis marrón y verde, Ginkgo biloba e Isoflavin Beta®) y el patrón usado (quercetina) mostraron actividad antioxidante de una manera dosis-dependiente con valores de IC 50 que van de 0,21 a 155,28 al μg mL-1 (inhibición de la peroxidación lipídioca y captación de radicales libres del DPPH •). Se observó una alta correlación (r 2 = 0,9913) entre los métodos antioxidantes y por otro lado la actividad antioxidante no estuvo relacionada con el contenido del polifenoles ni de flavonoides. Como el análisis del DPPH • es un método rápido, presenta costos bajos e incluso tiene una correlación alta con otros métodos antioxidantes, este método podría utilizarse como un parámetro adicional en el control de calidad de extractos naturales.