Galectin-3 enhances cyclin D1 promoter activity through SP1 and a cAMP-responsive element in human breast epithelial cells (original) (raw)
Galectin-3 is a multifunctional carbohydrate-binding protein found in the nucleus, cytoplasm and the extracellular milieu. Nuclear galectin-3 expression is associated with cell proliferation, and its role in pre-mRNA splicing has been suggested. In this report, we investigated the role of galectin-3 on cyclin D 1 gene expression, a critical inducer of the cell cycle and a potential oncogene in human cancer. We found that galectin-3 induces cyclin D 1 promoter activity in human breast epithelial cells independent of cell adhesion through multiple cis-elements, including the SP1 and CRE sites. We present evidence that galectin-3 induction of the cyclin D 1 promoter may result from enhancement/ stabilization of nuclear protein-DNA complex formation at the CRE site of the cyclin D 1 promoter. We also show that galectin-3 cooperates with, but does not depend on, pRb for cyclin D 1 promoter activation. The present study reveals a growth promoting activity of galectin-3 through cyclin D 1 induction, and suggests a novel function of nuclear galectin-3 in the regulation of gene transcription.
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