Effects of Plant Growth Regulators on Callus Formation in Different Explant of Calendula officinalis L (original) (raw)
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Annals of Biological Research, 2017
In the first stage of our research, C. officinalis and C. arvensis plants having medicinal and economical important were grown from embryo in the MS0 nutrient medium. In the second stage, callus culture was started with leaf explants of C. officinalis and C. arvensis plants and optimized with measuring of biomass in the MS0, MS1, MS3, MS4, MS6 nutrient mediums which were supplemented with combination of different concentrations of auxin and cytokinin. The statistical analysis relating to this measurements of callus biomass was conducted. The most favorable nutrient medium was established to be MS1 (1mg/l NAA+1mg/l BAP) for both C. officinalis and C. arvensis species during four different subculture from the 0. day. The most effective MS nutrient medium was determined to be MS1, MS4, MS3, MS6 and MS0, respectively.
An investigation of tissue culture and co-cultures of different explants in Calendula officinalis
Two experiments were conducted separately to investigate the effects of the medium and explant types on the callus induction and regeneration of Calendula officinalisas a medicinal plant. In the first experiment, explants were placed on a ΒΌ MSmedium on three levels of NAA (1-Naphthaleneacetic acid) and KIN (Kinetin) in sterile conditions. The parameters, time to callus initiation, callus volume, and the percentage of callus induction were measured to evaluate the callus condition in this experiment. Variance analysis of the results on the first experiment showed that there was no considerable difference in terms of callus induction in mediums with a combination of hormones, while the effect of explant source on these traits was significant. In the second experiment (co-culturing of different explants), main effects of cotyledon, hypocotyl and meristem explants as single cultures, and also effects of cotyledon and meristem, hypocotyl and meristem explants as co-cultures in the two me...
The present investigation was carried out during 2015-16 at research field of department of horticulture, Allahabad School of Agriculture, SHIATS, Allahabad. The experiment included thirteen treatments and three replications. Treatment used were T0 (Control), T1 (GA3 @100ppm), T2 (GA3@200ppm), T3 (GA3@300ppm), T4 (NAA@50ppm), T5 (NAA@ 100ppm), T6 (NAA@150ppm), T7 (NAA@200ppm), T8 (CCC@250ppm) and T9 (CCC@500ppm), T10 (CCC@750ppm), T11 (CCC@1000ppm), T12 (CCC@1250ppm). The results obtained showed that application of GA3 @300 ppm was found to be superior in plant height(38.6cm), plant spread(45.7cm), number of branches(34.4), number of leaves(240.7), and diameter of flower(6.8 cm), whereas, NAA @200 ppm was found to best for weight of flower(4.5 g),number of flowers per plant(42.2),yield of flowers per plant(211.3 g),yield of flowers per plot(1056.2 g) and yield of flowers (19.3 t/ha) as compared with control. Therefore, it can be concluded that application with GA3@300and NAA @200 ppm was found best for calendula growth and flower yield attributes.
2019
Abstract: The cell suspension culture C. officinalis and C. arvensis plants having medicinal and economical important was carried out in the four different MS medium which supplemented with different concentrations of NAA:BAP (1:1, 0.5:5 mg/l) and IAA:BAP (1:1, 0.5:5 mg/l)under sterile conditions. For this purpose, four months old calli which grown in the callus cultures were passed into the cell suspension culture. In this cell suspension cultures, cell counting and measurement of fresh/dry weight process were realized for two Calendula species during 40 days. This process repeated once in a five days. All of the research results were evaluated with Tukey Multiple Comparison Test.