Glucocorticoid-binding proteins in rat liver (original) (raw)

European journal of respiratory diseases. Supplement, 1982

Abstract

The distribution, metabolism and protein-binding of [3H] corticosterone in the rat liver was studied with respect to time and sex. The maximum recovery from the cell nuclei occurred 5 min after injection of the steroid. At this time, ten times more radioactivity was recovered from the liver cell nuclei of male animals compared to females. The recovered radioactivity in the cell nuclei was identified as mainly unmetabolised corticosterone and 5 alpha-dihydrocorticosterone. The radioactivity bound to the cytosolic glucocorticoid receptor protein also appeared to consist of unmetabolised corticosterone and 5 alpha-dihydrocorticosterone. However, 5 alpha-dihydrocorticosterone was found to have very little biological activity. The glucocorticoid receptor was found to exist in two forms with Stokes radii of 6.1 and 3.6 nm, respectively, following in vivo labelling with [3H] dexamethasone. The 6.1 nm form could be converted into the 3.6 nm form by incubation with trypsin, papain, alpha-chy...

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