Comparison of spch epidermis grown in a bio-imaging chamber and on plates (original) (raw)
2018
Abstract
<p>(<b>A</b>, <b>B</b>) Model outputs when leaf has grown to a width of about 0.5 mm (see horizontal line in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2005952#pbio.2005952.s014" target="_blank">S14A Fig</a>). (<b>A</b>) Epidermal model used to generate <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2005952#pbio.2005952.g008" target="_blank">Fig 8G</a>, corresponding to growth in a chamber. (<b>B</b>) Output from epidermal model tuned to match <i>spch</i> growth on plates (by slowing growth by 40% and physiological time by 45%). <b>(C)</b> Leaf grown in a bio-imaging chamber when width has attained 0.46 mm (8DAS). <b>(D)</b> Leaf grown on plates when width has attained 0.48 mm (13 DAS). <b>(E)</b> Enlargement of region indicated by magenta square in C. (<b>F</b>) Enlargement of region indicated by orange square in D. <b>(G,H)</b> Segmented cells from leaves shown in C,D. Cell area colour coded as heat map for A,B. <b>(I)</b> Enlargement of segmented region indicated by magenta square in G; average cell area 123.3 ± 6.4 μm<sup>2</sup> (<i>n</i> = 184). All cells with their centroid falling within the square were taken into account. <b>(J)</b> Enlargement of segmented region indicated by orange square in H; average cell area 199.8 ± 17.3 μm<sup>2</sup> (<i>n</i> = 111). <b>(K,L)</b> Cell complexity from leaves shown in C,D, quantified through the CD of the LOCO-EFA components of each individual cell’s shape, normalised for cell area (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2005952#sec019" target="_blank">Materials and methods</a>). Heat map range corresponds to zero for perfect circular or elliptical shapes, ranging to 5 as more LOCO-EFA harmonics are needed to describe the shape (dimensionless measure). (<b>M</b>) Enlargement of region indicated by magenta square in K. (<b>N</b>) Enlargement of region indicated by orange square in L. Scale bar = 100 μm. Source data are available from <a href="https://figshare.com/s/b14c8e6cb1fc5135dd87" target="_blank">https://figshare.com/s/b14c8e6cb1fc5135dd87</a>. CD, cumulative difference; DAS, days after stratification; LOCO-EFA, Lobe-Contribution Elliptic Fourier Analysis; <i>spch</i>, <i>speechless</i>.</p
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