Comparative Study of Ability for Growth Support and Species Differentiation by Colony Features on Commercial Chromogenic Agar, Pourmedia Vi Candida and CHROMagar Candida (original) (raw)
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The growth dynamic model of Typha angustifolia and Typha latifolia was developed by considering the characteristics of initial biomass allocation and their energy transport from below to aboveground organ while heterotrophic growing season. Growth characteristics of Typha spp. were investigated by sampling their each organ in their early growing stage. Initial relationships among belowground biomass, aboveground biomass and shoot height were investigated. Typha spp. have less capacity for utilizing the reserved material than Phragmites australis. Shoot elongation is seasonally changed in each species and the highest at start of growth. The vertical productive structures of Typha spp. were not so change seasonally. By using above obtained characteristics, the model was successfully applied to simulate the seasonal variation of the total biomass with different water depth for Typha angustifolia and Typha latifolia.
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Four cestode samples collected from four patients were identified by homology searching of the partial mitochondrial cytochrome oxidase subunit 1 gene (COX1) sequences. The COX1 region was amplified by PCR using a pair of JB3 and JB4.5 or DBCOI-1 and DBCOI-2 primers for the Pseudophyllidea cestode and by multiplex PCR for the Cyclophyllidea cestode. The COX1 sequences of two samples speculated to be Diphyllobothrium nihonkaiense and D. latum were morphologically identical and homology searching showed that the sequences of the two samples had 99.6% to 100% identity with those of D. nihonkaiense. The two samples were therefore identified as D. nihonkaiense. Similarly, a sequence from a larva isolated from the great omentum of one patient showed 98% to 100% identity with that of Spirometra erinaceieuropaei. This sample was therefore identified as larval S. erinaceieuropaei. The remaining sample tested positive for Taenia saginata in multiplex PCR and the sequence from the diagnostic fragment had 99.7% to 100% identity with that of T. saginata. This sample was therefore identified as T. saginata. The present findings highlight the usefulness of genetic examination for the diagnosis of cestodiasis.