In vitrosusceptibility ofPrototheca zopfiigenotypes 1 and 2 (original) (raw)
(GM), kanamycin (KM) and itraconazole (ITZ) [6-10]. However, in vitro susceptibility tests on the two P. zopfi i genotypes have not been well investigated. This is the fi rst study to assess the susceptibility of genotype 2 isolates from bovine mastitis and genotype 1 strains recovered from cow-barn surroundings. Materials and methods Strains The type P. zopfi i strain of genotype 1 (SAG2063 T) and genotype 2 (SAG2021 T) were used for susceptibility tests. In addition, 10 isolates of P. zopfi i genotype 2 were recovered from 10 cases of bovine protothecal mastitis in Japan. Ten isolates of P. zopfi i genotype 1 were obtained from Japanese farm stock [3]. These isolates were previously defi ned by an 18S rDNA-based genotype-specifi c PCR assay [3-5] (Table 1).
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