"Saccharomyces cerevisiae"}} (original) (raw)

The yeast trehalose-6-phosphate synthase (Tps1) catalyzes the formation of trehalose-6-phosphate (T6P) in trehalose synthesis. Besides, Tps1 plays a key role in carbon and energy homeostasis in this microbial cell, as shown by the well documented loss of ATP and hyper accumulation of sugar phosphates in response to glucose addition in a mutant defective in this protein. The inability of a mutant to cope with fermentable sugars is still a matter of debate. We reexamined this question through a quantitative analysis of the capability of homologues from different origins to complement phenotypic defects of this mutant. Our results allowed to classify this complementation in three groups. A first group enclosed of with that of as their expression in cells fully recovered wild type metabolic patterns and fermentation capacity in response to glucose. At the opposite was the group with homologues from the bacteria and , the plant and the insect whose metabolic profiles were comparable to t...