Serotyping, RAPD PCR, Antibiotic Susceptibility Profiling of Listeria spp. Isolated from Raw Chicken Meat (original) (raw)
Related papers
2020
In this study, 240 organic chicken pieces (80 thighs, 80 wings, 80 skinless-breast meat) were analyzed for the presence of Listeria monocytogenes. Within the framework of the analysis findings; L. monocytogenes was detected in 60 (25%) of all 240 collected samples. In particular, L. monocytogenes was detected in 24 (30%) of 80 thigh samples, 20 (25%) of 80 wing samples and 16 (20%) of 80 skinless-breast meat samples. Serotyping distribution of 96 L. monocytogenes isolates determined as 71.8% serotype 1/2a, 21.9% serotype 1/2b, 4.2% serotype 4b and 2.1% serotype 1/2c. According to antibiotic resistance profile, 26 isolates (27%) were found to be resistant to ampicillin. The other isolates were found to be resistant to meropenem, tetracycline, sulfamethoxazole/trimethoprim, penicillin G, amoxicillin/clavulanic acid, vancomycin, oxytetracycline, erythromycin and chloramphenicol as 23 (23.9%), 14 (14.5%), 13 (13.5%), 12 (12.5%), 9 (9.3%), 7 (7.2%), 5 (5.2%), 4 (4.1%) and 3 (3.1%) respec...
L.monocytogenes is wide spread in the environment and can be isolated from a wide variety of food. As such it can be expected to be present in ready-to-eat foods that contain raw ingredients. The risk caused by L. monocytogenes is dependent on the food and how long it is stored. To estimate the incidence and levels of Listeria spp. in different food sources, A total of 200 random samples which collected from different sources 65 samples isolated from poultry , 45 from poultry byproducts (Frozen chicken meat balls (kofta), Frozen chicken burger and frozen chicken sausages), 20 from pasteurized milk ,30 from milk products (Feta cheese ,cream cheese )and 40 from poultry eggs .Only 7(14%) of samples that collected from suspected isolates and detected on Oxford agar, PALCAM agar and ALOA agar were suspected to be Listeria spp. The in-vitro antimicrobial sensitivity test showed that the isolated L.monocytogenes were sensitive to Sulphamethoxazole /trimethoprim, Gentamycin ,Ceftazidime and Cephazoline followed by Sulphamethoxazole, ceftriaxone , weak sensitivity to Penicillin , While they were resistant to Erythromycin, Clindamycin, Ampicillin. All L.monocytogenes were virulent strains as all of them were positive to CAMP test; showed narrow zone of β?hemolysis on sheep blood agar .The PCR results for Listeria isolates showed that all strains are L.monocytogenes and detect 16SrRNA gene and have mefA ,tet M and ampC genes (100%) as resistance genes while Cat and Aad6 genes detected as 85.7% and 71.4% respectively.
Infection and Drug Resistance, 2008
This study was carried out on 426 samples of raw meats collected from butcheries and supermarkets in Casablanca, Morocco. The samples were examined for the occurrence of Listeria species. Strains of Listeria monocytogenes were characterized by several biochemical tests and confi rmed by polymerase chain reaction (PCR). β-hemolytic cultures and nonhemolytic isolates were tested for biochemical properties with the Listeria API test. Among the 43 Listeria species isolates; we identifi ed 10 strains for L. monocytogenes (23.3%), 31 strains for L. innocua (72.1%) and 2 strains for L. welshimeri (4.6%). Strains of L. monocytogenes were separated by multiplex PCR; two serogroups IIb and IVb were thus differentiated. Antibiotic susceptibility of L. monocytogenes to 21 antibiotics was determined by the disk diffusion method. All isolates were susceptible to a wide range of the tested antibiotics with the exception of nalidixic acid, colistine and cephalosporins second and third generation for which they were all resistant.
Listeria monocytogenes is a ubiquitous microorganism in nature and is responsible for listeriosis which is an infectious disease caused by consumption of contaminated food. It is responsible for a rare but serious disease in poultry. The objective of this study was to determine the prevalence ratio of listeria species in chicken and chicken byproducts and discuss the detection of some virulence and resistance genes among Listeria isolates. Bacteriological examination of 120 samples collected from chickens and chicken byproducts revealed a low recovery rate of L. monocyotogenes isolates (1.1%) in chickens whereas it was isolated in a ratio of 20% and 13.3% out of 30 chicken byproduct samples (15 burger and 15 luncheon). Antimicrobial susceptibility testing of L. monocyotogenes isolates demonstrated that all isolates were highly sensitive. Meanwhile, the highest resistance (54.5%) rates were recorded against tetracycline followed by the resistance to penicillin and nalidixic acid (45.5% for each). All isolates were identified using 16S rRNA gene as L. monocytogens by PCR technique in addition to the molecular detection of some virulence and antibiotic resistance genes of the isolates will be discussed in details. Overall, a recommendation with good hygienic measures should be enforced in order to minimize presence of L. monocytogenes in chicken and in chicken byproducts during manufacturing, handling and storage process at plant and retail stores level.
Significance and Characteristics of Listeria monocytogenes in Poultry Products
International Journal of Food Science
Listeria monocytogenes is one of the most common foodborne pathogens. Poultry meat and products are of the main vehicles of pathogenic strains of L. monocytogenes for human. Poultry products are part of the regular diet of people and, due to nutrient content, more content of protein, and less content of fat, gain more attention. In comparison with red meat, poultry meat is more economical. So, it had a greater rate of consumption especially in barbecue form in which the growth of bacterium is favored. Subtyping of L. monocytogenes isolates is essential for epidemiological investigation and for identification of the source of contamination. In the following review, the main facet of presence of L. monocytogenes in poultry will be discussed. Most pathogenic serotypes of L. monocytogenes were detected in different products of poultry meat. Unfortunately, these isolated pathogens had sometimes resistance to commonly used antibiotics which were used for treatment of human infection.
British Microbiology Research Journal, 2016
Background: Listeriosis, mostly caused by Listeria monocytogenes species, has become a major concern to public health authorities due to its clinical severity and high mortality rate, particularly in high risk groups. Currently, there is limited information regarding the prevalence and antimicrobial susceptibility profiles of listeria species in ready-to-eat foods of animal origin in Gondar town, Ethiopia. The aim of this study was to determine the prevalence and antimicrobial susceptibility pattern of Listeria species isolated from ready-to-eat food of animal origin from public dinning places in Gondar town, Ethiopia. A cross sectional study on ready-toeat foods of animal origin sampled from major supermarkets, butcher shops, pastry shops, restaurants and hotels was carried out. Culture, biochemical and sugar tests were conducted for listeria species identification and disc diffusion test was performed to study the antimicrobial susceptibility profiles of the isolates. Results: Out of 384 food samples examined, 96 (25%) were positive for Listeria species. Listeria monocytogenes was detected in 24 (6.25%) of the samples. Listeria monocytogenes was isolated from cake, raw meat, ice cream, minced beef, fish, unpasteurized milk and pizza in that order from higher to lower rate. Assessment of antimicrobial susceptibility profile of L. monocytogenes revealed the presence of four multi-drug resistant isolates. The higher resistance rate was recorded for penicillin, nalidixic acid, tetracycline and chloramphenicol, in decreasing order. All L. monocytogenes identified in the current study were sensitive to amoxicillin, cephalothin, cloxacillin, sulfamethoxazole, gentamicin and vancomycin. Conclusions: The presence of L. monocytogenes including drug resistant and multidrug resistant isolates in some ready-to-eat food items is an indicator of the presence of public health hazards to the consumer, particularly to the high-risk groups. Hence awareness creation on food safety and implementation of regulations about the use of drugs in humans and animals is strongly recommended.
Journal of Istanbul Veterinary Sciences
Listeria species are widespread in nature and found in various environments. In this study, the presence of Listeria species in poultry farms was investigated. For this purpose, a total of 332 samples including feces, feed, drinking water and nipple swab from 5 broiler flocks reared on the floor and 352 samples including feces, feed, drinking water and eggshell swab from 5 layer flocks reared in cages were obtained. A modified version of the USDA-FSIS MLG 8.13 method was used for the isolation of Listeria species from samples. As a result of the study, Listeria spp. was isolated from all broiler and layer flocks. Isolation of Listeria spp. was carried out from 18 of 190 feces samples (9.4%), 5 of 15 drinking water samples (33.3%), 3 of 102 nipple swab samples (2.9%) in broiler flocks, and 22 of 167 feces samples (13.1%), 2 of 25 feed samples (8%), 3 of 15 drinking water samples (20%), 3 of 145 eggshell swab samples (2%) in layer flocks. Isolates were identified by cultural and bioch...
Bioresearch Communications
Ready-to-eat (RTE) meat products are food items that are extremely popular worldwide. Albeit documented with the alarming prevalence of pathogenic multidrug-resistant Listeria species in these food items worldwide, studies from the perspective of Bangladesh are absent. This study was devised with the aim to detect pathogenic Listeria monocytogenes in some of the most popular RTE meat products in Dhaka, Bangladesh. Thirty-nine such samples were investigated using biochemical tests and the Polymerase Chain Reaction (PCR) analysis. Following confirmatory tests and detection of Listeria species, the isolates were subjected to the Kirby-Bauer disc diffusion test to investigate their antibiotic-susceptibility patterns against some of the most commonly used antibiotics to treat listeriosis – the infection caused by Listeria. Five Listeria species were detected using biochemical and PCR tests. This constituted an overall prevalence rate of 12.8% (n = 39). Four out of the five Listeria speci...
Foods, 2020
Listeria monocytogenes are the etiological factor of listeriosis, and their main source for humans is food. The aim of the current study was to assess the contamination of various types of meat and the drug susceptibility of isolated L. monocytogenes. Between 2016–2018, 6000 swabs were taken (2000 annually) from the surface of pork, beef, and poultry. The analysis of intermediate and finished product samples was carried out in accordance with ISO 11290-1 (International Organization for Standardization). The genetic similarity assessment of the isolates obtained was based on the Pulsed Field Gel Electrophoresis (PFGE) method, and drug-sensitivity assessment using the disc-diffusion method. We found 2.1% of collected samples were L. monocytogenes positive. The level of meat contamination varied depending on its matrix. Most L. monocytogenes were isolated from poultry. It was shown that 39 (32.5%) strains were sensitive to all tested antibiotics and eight (6.7%) were resistant to all f...
Prevalence of Listeria monocytogenes in Beef , Chevon and Chicken in Bangladesh
2016
The present research was conducted for the isolation and characterization of the Listeria monocytogenes from meat samples collected from different local markets around Mymensingh town such as Boira, Paglar bazar, Kawatkhali bazar, Kamal-Ranjit (K-R) market and Shes More bazar. A total of 36 meat samples were collected from cattle, goat and chicken. After preparation the samples were inoculated into different selective media such as Oxford Agar, Mannitol Salt Agar and Blood Agar for isolation and identification of L. monocytogenes. In Oxford Agar, Listeria spp. produced black zone around the colonies. Gram staining, motility test, blood hemolysis, Christe Atkins Munch Peterson (CAMP) test and biochemical test were performed to confirm L. monocytogenes. The L. monocytogenes fermented dextrose and maltose with the production of only acid and no gas was observed in Durham′s tube. The L. monocytogenes were found Methyl-Red and Voges-Proskauer (MR-VP) test positive but indole negative. L....