Fabrication of CuONPs using Tridax Procumbens Leaf Extract as Material Antibacterial with Green Synthesis Method (original) (raw)
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RSC Advances, 2023
Antimicrobial resistance (AMR) resulting from indiscriminate use of antibiotics in various fields of agriculture such as livestock farming, aquaculture, and croup fields become an emerging catatroph for the health (human, animal) and environment. Among those, poultry farming has been considered as one of the major contributors of multidrug-resistant (MDR) bacteria. Focusing this, the present research is designed for green synthesis of copper oxide nanoparticles (CuONPs) with the aim of their application in antibiotic-free poultry farming for curving use of antibiotics in that sector. For that, antibacterial CuONPs were nanoformulated to decrease the required doses of bulk CuSO4. We used a CuSO4$5H2O solution as a Cu2+ source and Citrus limon juice as a reducing agent as well as capping agent. Particle yield was initially confirmed by the lmax specific to CuONPs (295 nm) using UV-Vis spectroscopy. The presence of the Cu–O group during particle formation and crystallinity with the purity of yielded NPs was confirmed with Fourier-transform infrared spectroscopy and X-ray diffractometry. The round to spherical CuONPs of 92–155 nm average size was confirmed with atomic force, scanning electron, and transmission electron microscopy. The concentration of yielded NPs was calculated with the dynamic light scattering. The physical characterization tools indicated a maximum CuONPs yield with a 0.001 M ion source with 15% reducing agents after 12 h reduction. Antibacterial effectivity was tested against methicillin-resistant Staphylococcus aureus and tetracycline- and beta-lactamase-resistant Escherichia coli, confirmed by PCR amplicon band at 163 bp, 643 bp, and 577 bp for the mecA, blaTEM-1 and tetA genes, respectively. An antibiogram assay of CuONPs showed a maximum zone of inhibition of 26 ± 0.5 mm for the synthesized particles. The minimum inhibitory and bactericidal concentrations were 1.6 mg ml−1 and 3.1 mg ml−1 , respectively, for broadspectrum application. Finally, the biocompatibility of CuONPs was determined by demonstrating a nonsignificant decrease of BHK-21 cell viability at <2 MIC doses for complying their future in vivo applicability.
Novel Route of Synthesis of PCL-CuONPs Composites With Antimicrobial Properties
Dose-Response
Nanoparticles of metals can be toxic to bacteria, showing biocidal activities at low concentrations. Metal, oxide, or compounds based on copper are applied like antimicrobial agents. The capacity of integration of metallic nanoparticles in polymer matrices has improved the antimicrobial behavior, resulting in the search for composites with increased bactericidal properties. A polycaprolactone (PCL) film polymer with copper oxide nanoparticles (CuONPs) was prepared. Dynamic light scattering analysis showed the sizes from 88 to 97 nm of CuONPs. Scanning electron microscopy (SEM) revealed CuONPs with semispherical shapes with diameter 35 nm. The prepared PCL-CuONPs exhibited a nanoporous structure by SEM. The antibacterial applicability of the composite was evaluated to determine the minimum inhibitory concentration in 6 different bacteria and the experimental tests were carried by disk diffusion and spectrophotometric methods. The PCL-CuONPs exhibit a considerable antibacterial effect...
ChemClass Journal , 2024
Nanotechnology, a rapidly evolving field, focuses on designing, synthesizing, and utilizing materials at the nanoscale, offering revolutionary applications across industries. This study investigates the synthesis, characterization, and application of nanoparticles for specific functional applications, employing ultraviolet-visible (UV-Vis) spectroscopy, Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) for detailed analysis. Nanoparticles were synthesized through the green method and subjected to comprehensive characterization. UV-Vis spectroscopy was used to evaluate the optical properties, confirming the surface plasmon resonance (SPR) indicative of nanoparticle formation. FTIR analysis identified functional groups and chemical interactions, while SEM provided detailed morphological insights and subjected to antimicrobial screening. UV-Vis spectra confirmed a characteristic SPR peak at 250 nm, correlating with the desired particle size and uniformity. FTIR analysis revealed FTIR analysis identified functional groups, including hydroxyl (-OH), carboxyl (C=O), and amine (C-N), which reflect the presence of flavonoids, phenols, and proteins from the Vernonia amygdalina extract. These functional groups likely play a vital role in reducing and stabilizing Cu ions, enhancing nanoparticle bioactivity, and highlighting successful chemical bonding and stability of the nanoparticles. SEM images depicted uniform morphology and size distribution with an average particle size of 20nm, supporting consistent synthesis. CuONPs showed minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against several organisms: Staphylococcus aureus (S.a) (MIC 6.25 µg/ml, MBC 12.5 µg/ml), Bacillus subtilis (B.s) (MIC 3.125 µg/ml, MBC 50 µg/ml), Escherichia coli (E.c) (MIC 12.5 µg/ml, MBC 50 µg/ml), and others. Compared to controls like ciprofloxacin, CuONPs demonstrated broader efficacy, especially against resistant strains like Pseudomonas aeruginosa (PS.a) (MIC 6.25 µg/ml).
Journal of Pharmacy and Pharmacology, 2019
Copper oxide nanoparticles (CuO NPs) have attracted huge attention due to catalytic, electric, optical, photonic, and antibacterial activity. The use of plant leaf extracts in the biosynthesis of nanostructured materials is an eco-friendly, non-toxic and cost effective approach. Hence, this study aims to provide an updated survey of the main green synthesis method of CuO NPs using leaf extract of Khat, its characterization and studies on antibacterial activities. The synthesis of CuO NPs was done by reducing 1 mM CuSO 4 •5H 2 O with 2% (m/v) aqueous leaf extract of Khat under optimum conditions (pH = 11). The formation of CuO NPs has been confirmed first by the color change from colorless to light green and then to greenish yellow. The kinetics of the reaction was studied using UV-Visible (UV-Vis) spectrophotometer that showed surface plasmon resonance at 333 nm. The CuO NPs exhibit antimicrobial activity against two human pathogens viz. Salmonella typhimurium and Escherichia coli with an average zone of inhibition of 12.5 mm and 8 mm, respectively. Therefore, the current study reveals a convenient utilization of Catha edulis extract as a reducing agent for the successful synthesis of CuO NPs through a green synthesis method to obtain significantly active antibacterial material.
International Journal of Engineering Research and Technology (IJERT), 2014
https://www.ijert.org/green-synthesis-of-cuo-nanoparticles-using-phyllanthus-amarus-leaf-extract-and-their-antibacterial-activity-against-multidrug-resistance-bacteria https://www.ijert.org/research/green-synthesis-of-cuo-nanoparticles-using-phyllanthus-amarus-leaf-extract-and-their-antibacterial-activity-against-multidrug-resistance-bacteria-IJERTV3IS040918.pdf In the present study, we report biologically oriented process for green synthesis of CuO nanoparticles by using eco friendly and non-toxic Phyllanthus amarus leaf extract. Powder X-Ray Diffraction (XRD) analysis revealed that synthesized CuO nanoparticles are in monoclinic phase with average particle size of 20 nm. The antibacterial activity of these nanoparticles was tested against various multidrug resistance bacteria viz. both Gram-positive (B. subtilis and S. aureus) and Gram-negative (E.coli and P. aeruginosa). Further, these nanoparticles show higher antibacterial activity against B. subtilis followed by S. aureus, P. aeruginosa and E.coli.
Research Square (Research Square), 2022
A fast, e cient, simple and environmentally-friendly, green synthesis method was employed for the synthesis of copper oxide nanoparticles (CuONPs) using easily available, low-cost and non-toxic Justicia Schimperiana plant leaf extract (JSPLE) from Ethiopia. Ultraviolet-Visible (UV-Vis) spectrophotometer, Xray Diffraction (XRD) technique and Fourier-Transform Infrared (FTIR) Spectroscopy were used to characterize the synthesized CuONPs. The UV-Vis con rmed the formation of CuONPs and the maximum absorbance for the synthesized CuONPs in solution at 212 nm. The different parameters like penta hydrated copper sulphate concentration, volume of leaf extract, temperature, pH and time were optimized spectrophotometrically. The FTIR analysis con rmed the existence of various functional groups responsible for reducing and stabilizing during the biosynthesis process. The XRD result con rmed that the synthesized CuONPs were crystalline in nature with the monoclinic structure and the average crystallite sizes of the particles were 21.8 nm. The synthesized CuONPs showed antimicrobial (gramnegative bacteria (Salmonella typhimurium,Escherichia coli and Klebsiella pneumoniae) and grampositive bacteria (Staphylococcus aureus)) activity. It has been noted that the green synthesized CuONPs showed e cient antibacterial activity against gram-negative (salmonella typhimurium) bacteria with maximum ZOI of 18.6 mm with respect to the highest concentration (150 μg/mL). The developed method for the CuONPs synthesis using JSPLE is an eco-friendly and convenient method. In the near future, the synthesized CuONPs could be used in the elds of biomedicine, biosensor, water treatment and nanotechnology.
Green synthesis and characterisation of CuNPs: insights into their potential bioactivity
IET Nanobiotechnology, 2018
The current investigation involves the green synthesis of copper nanoparticles (CuNPs) from an aqueous plant extract of Moringa oleifera Lam by two methods: (I) time-based approach and (II) heat treatment of aqueous solution. Prepared CuNPs were characterised via Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy and transmission EM. The study also reveals the potential bioactivity of the prepared CuNPs. In vitro anti-microbial efficiency of CuNPs was estimated against bacterial and fungal strains by the agar well diffusion method. Anti-oxidant capacity of CuNPs was determined using ferric reducing ability of plasma (FRAP), lipid peroxidation (LPO) and peroxidase assays, while the antiplatelet potential was determined by measuring two haemostatic parameters (PT & APTT assay). The minimum inhibitory concentration was observed at 60 µg/ml against Streptomyces griseus and Aspergillus niger when NPs were prepared by method II. CuNPs prepared by the method I showed higher FRAP and LPO activities, while increased POX activity was found in CuNPs prepared by method II. CuNPs prepared using method I also showed better anti-oxidant and antiplatelet potential. It was observed that M. oleifera-derived CuNPs exhibits strong anti-microbial, anti-oxidant and APTT potential. This indicates potential utilization of green synthesized NPs for various industrial and therapeutic strategies.
2021
The present research work focused on the synthesis, characterization and testing of antimicrobial activity of CuO nanoparticles prepared by green synthesis method using Trigonella foenum-graecum L. leaf extract. The XRD pattern indicated the presence of cubic structure of nanocrystalline CuO with crystallite size of 47 nm. The optical band gap was calculated using Tauc’s plot and found to be 3.6 eV. SEM images revealed that the particles had cluster structures with a combination of different shapes. EDAX analysis confirmed the presence of copper and oxide by indicating predominant peak which was the highest percentage present in the spectrum. The synthesized nanoparticles showed significant antibacterial and antifungal activity against the human pathogens Staphylococcus aureus and Penicillium sp. So this nanoparticle can be considered as an alternate approach for reducing the adhesion of micro-organisms.
IET Nanobiotechnology, 2017
In present investigation, Copper oxide (CuO) nanostructures have been prepared via green chemistry. Olea europaea leaf extract act as strong chelating agent for tailoring physical as well as bio-medical characteristics of CuO at the nano-size. Physical characterization such as Scanning electron microscope (SEM) analysis depicts the formation of homogenized spherical shape nanoparticles with average size of 42 nm. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) further confirmed the crystalline pure phase and monoclinic structure. High performance liquid chromatography (HPLC) testing is performed to evaluate the relative concentration of bioactive molecules in the Olea europaea leaf extract. From HPLC results capping action of organic molecules around CuO-NPs is hypothesized. The antimicrobial potency of biosynthesized CuO nanoparticles (CuO-NPs) have been evaluated using colony forming unit (CFU) counting assay and disc diffusion method which shows a significant zone of inhibition (ZOI) against bacterial and fungal strains may be highly potential for future antimicrobial pharmaceutics. Furthermore, reduction of various precursors by plant extract will reduce environmental impact over chemical synthesis.