Multifocal fluorescence video-rate imaging of centimetre-wide arbitrarily shaped brain surfaces at micrometric resolution (original) (raw)

This paper presents a novel technique for wide-field fluorescence imaging that addresses the challenges of capturing rapid cellular dynamics on the curved surfaces of the brain. The method employs a spinning disc with varying thickness for selective illumination, achieving video-rate imaging at micrometric resolution over centimetre-wide, arbitrarily shaped surfaces. By demonstrating the capability to acquire images at exceptionally high speeds, the technique is positioned as a powerful tool for studying multiscale cellular interactions in live animal models.