Effect of topical nitric oxide donor (0.2 % glyceryl trinitrate) on wound healing in diabetic wistar rats (original) (raw)

International Journal of Diabetes in Developing Countries, 2013

Abstract

ABSTRACT Cutaneous wound healing activity of 0.2 % glyceryl trinitrate (GTN) ointment was evaluated in diabetic wistar rats. Streptozotocin (STZ)-induced diabetic rats were divided in four groups (N = 6) and anaesthetized by ketamine 75 mg/kg and xylazine 10 mg/kg. Two groups were operated for excision wound (EW). One group served as control and another as 0.2 % GTN ointment treatment group. EW groups were studied for wound closure rate and re-epithelization. Remaining two groups were divided into control and treatment group. Rats in these two groups were inflicted with incision wound (IW) and dead space wound (DW) in the same animal. IWs were studied for wound tensile strength while DWs were studied for granulation tissue weight, hydroxyproline content and histological changes in granulation tissue. EW showed significantly higher re-epithelization in treatment group compared to control (61.10 ± 2.98 vs 27.36 ± 6.68; P = 0.01) but there was no difference in wound closure rate between the groups. 0.2 % GTN did not affect tensile strength of IW, granulation tissue weight or hydroxyproline content. This suggests that topical NO does not have any effect on collagen secretion and maturation by fibroblasts in incisional wound model. In conclusion, NO donor may useful in the wounds where wound contraction has a minor role in wound closure e.g., diabetic foot ulcers.

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