Low-level laser on femoral growth plate in rats (original) (raw)
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The Effects of Low-Level Laser Therapy, 670 nm, on Epiphyseal Growth in Rats
The Scientific World Journal, 2012
The longitudinal growth of long bones is attributed to epiphyseal growth. However, the effects of low-level laser therapy (LLLT) in such structures has still not been studied extensively in the literature. Therefore, the aim of this study was to evaluate the use of LLLT, 670 nm, at three different doses on the epiphyseal growth of the right tibia of rats. Twenty-one Wistar rats, aged four weeks, were subjected to the application of LLLT, with dosage according to the group (G4: were submitted to the application of 4 J/cm 2 ; G8: were submitted to the application of 8 J/cm 2 ; G16: were submitted to the application of 16 J/cm 2 ). After completion of protocol they were kept until they were 14 weeks of age and then submitted to a radiological examination (evaluation of limb length) and euthanised. The histological analysis of the growth plates (total thickness and hypertrophic and proliferative zones) was then performed. Comparisons were made with the untreated left tibia. No differences were observed in any of the reviews (radiological and histological), when comparing the right sides (treated) to the left (untreated). It was concluded that the treatment with LLLT within the parameters used caused changes neither in areas of the epiphyseal cartilage nor in the final length of limbs.
Analysing the Effect of Low Level Laser Therapy on Bone Histomorphometry in Rats
Recent Research Advances in Biology Vol. 11, 2021
The aim of this study was to assess the histological effect of Low Level Laser Therapy (LLLT) (904 nm) on the repair of standardized bone defects on the femur of rats. Sixty male wistar rats were assigned into two equal groups. Group (A: laser group) and group (B: control group). A surgical fracture was done in middle third of femur of all rats. In group (A) a continuous wave 904 nm infrared laser was applied at dose 4 j/cm 2 at fracture site immediately post operative for 7 sessions, each session was 5 minutes. The animals were killed by over dose of general anesthesia on the 15th,30th and 45th days after surgery, The specimens were processed and stained with Hematoxylin-eosin (H/E), special stain Masion trichrome and analyzed by light microscopy. The descriptive analysis of histological imaging showed greater degree of new bone formation, osteoblastic surface and collagen fiber in the irradiated group when compared with the control group. Based on the obtained results, this study concluded that LLLT was efficient in promoting bone healing, and increasing new bone formation in the process of surgically fractured femur in animal study.
Effect of Low Level Laser Therapy on Bone Histomorphometry in Rats
Life Science Journal, 2011
Abstract: The aim of this study was to assess the histological effect of Low Level Laser Therapy (LLLT) (904 nm) on the repair of standardized bone defects on the femur of rats. Sixty male wistar rats were assigned into two equal groups. Group (A: laser group) and group (B: control ...
Effect of irradiation of 810nm laser on bone for 10 sec: A rabbit histological study
IOSR Journals , 2019
Introduction and Objective In last decade, low level laser therapy has been evaluated for stimulation and acceleration of bone formation. In spite of promising results, biphasic ‘dose’ response remains. Moreover, the use of single session of low level laser on healing of bone is not explored thoroughly. The aim of this study was to determine the optimal ‘dosage’ for formation of bone using diode laser of 810nm under single irradiation. Materials and Methods Six New Zealand male rabbits were used weighing 1.5-2 Kgs and 8-10 months old for the study. Femur was chosen as site of surgery. The centre of the femur was drilled using implant osteotomy drills to the size of 2.8mm in width and 6mm in depth. 810nm Diode laser (GaAlAs, AMD Picasso®) was used in this study. Laser parameters were, wavelength of 810nm, power of 90mW, time of 10 seconds in continuous mode using the disposable fibre of 300µmm diameter in light noncontact. Contra lateral femur was used as a control and the laser was sham treated. At the end of 2 weeks samples were collected from the surgical area and slides were prepared and analysed histologically. Results At 14th day, the lased group showed slight areas of haemorrhage in marrow cavity with no evidence of bone formation in lased site. Conclusion The results of the present study using 810nm, 90mW, for 10 sec for single session did not stimulate formation of new bone in two weeks.
Laser therapy in bone repair in rats: analysis of bone optical density
Acta ortopedica brasileira, 2014
To investigate, by digital radiology, the bone regeneration process in rats submitted to femoral osteotomy and treated with low power laser therapy. Forty-five Wistar rats were subjected to transverse osteotomy of the right femur and divided randomly into three experimental groups (n = 15): animals not treated with laser therapy G (C), animals that received laser therapy with λ: 660nm G (660nm) and animals that received laser therapy with λ: 830nm G (830nm). Animals were sacrificed after 7, 14 and 21 days. The bone calluses were evaluated by digital X-ray at 65 kVp, 7mA and 0.032 s exposures. The values obtained were submitted to variance analysis (ANOVA) followed by the Tukey-Kramer test. The significance level adopted was 5%. The groups G (C), G (660nm), and G (830nm) at the 7(th) day showed a significant bone development, with p <0.0116; the groups G (C), G (660nm), and G (830nm) at the 14(th) day showed values of p <0.0001; at the 21(st) day,a higher degree of bone repair ...
Lasers in Medical Science, 2011
The present study aimed to evaluate the effects of LLLT (660-and 808-nm wavelengths) on the process of repairing bone defects induced in the femurs of female rats submitted to ovariectomy. Bilateral ovariectomies were performed on 18 female Wistar rats, which were divided into control and irradiated groups after the digital analysis of bone density showed decreased bone mass and after standardized drilling of the femurs. The irradiated groups received 133 J/cm 2 of AsGaAl (660-nm) and InGaAlP (880-nm) laser radiation. The animals were euthanized on days 14 and 21 after the bone defects were established.
Histologic study of the effect of laser therapy on bone repair
The journal of contemporary dental practice, 2008
This study used histologic analysis and HE staining to evaluate laser biomodulation of bone repair in cavities made in the femurs of rats that underwent non-ablative laser irradiation. Eighteen male Wistar rats weighing 300 to 400 grams were randomly assigned to three groups of six animals each. A surgical defect site was produced with a trephine about 2 mm in diameter under abundant irrigation. In Group I the complete surgical protocol to produce a bone defect was followed but without laser radiation (control). In Group II a continuous wave 830 nm infrared laser was used at 10 J/cm2 and 50 mW at each point of the surgical site. In Group III a continuous wave 685 nm infrared laser at 10J/cm2 and 35 mW was used at each point of surgical site. The animals were irradiated at intervals of 48 hours beginning immediately after the preparation of the defect and were sacrificed on the 15th, 21st, and 30th days. Slides were studied by means of descriptive analysis. Greater degrees of new bon...
IRANIAN JOURNAL OF …, 2008
Treating articular cartilage in dogs is a challenge in the contemporary orthopedics of small animals. Currently application of Low Level Laser being advocated for various orthopedics disorders that is why its effect being evaluated in experimentally induced defect in hip joint. The experiment was conducted on 24 adult male rabbits which were divided into two groups of (control & experimental) with 12 rabbits each. Effective anesthesia was induced using combination of acepromazine, ketamine and xylazine. After exposure and subluxation of right femoral head, the maximum accessible cartilage was denuded up to subchondral bone using dental bit in each rabbit. No treatment was given to control group, whereas experimental one was subjected to Low Level Laser Therapy daily for 1 minutes diode laser 630 nm and 2 minutes of infrared with 890 nm for 14 days daily, then further they were subdivided into 3 subgroup of 4 rabbits each with duration of 1 ,2 and 3 months. There was no trace of firbrocartilage or cartilage formation in control group samples even up to end of observation period .There was remarkable connective tissue with trace of chondrocytes beneath that which were located in the large lacunae having different type of isogenic during one month period in experimental group.. Scattered collagen fibers between chondrocyte cells was seen which was not enough to fill the defect in one month period, whereas drastic changes was noticed in two and three months duration with full coverage of defect area with newly formed cartilage
2002
Objective: The aim of this study was to evaluate morphometricly the amount of newly formed bone after GaAlAs laser irradiation of surgical wounds created in the femur of rats. Background Data: Low-level laser therapy (LLLT) has been used in several medical specialties because of its biomodulatory effects on different biological tissues. However, LLLT is still controversial because of contradictory reports. This is a direct result of the different methodologies used in these works. Materials and Methods: In this study, 40 Wistar rats were divided into four groups of 10 animals each: group A (12 sessions, 4.8 J/cm 2 per session, observation time of 28 days); group C (three sessions, 4.8 J/cm 2 per session, observation time of 7 days). Groups B and D acted as nonirradiated controls. The specimens were routinely processed to wax and cut at 6-mm thickness and stained with H&E. For computerized morphometry, Imagelab ® software was used. Results: Computerized morphometry showed a significant difference between the areas of mineralized bone in groups C and D ( p = 0.017). There was no difference between groups A and B (28 days; p = 0.383). Conclusion: It is concluded that, under this experimental condition, LLLT increased bone repair at early bone healing. 83
Effect of 650 nm low-power laser on bone morphogenetic protein in bone defects induced in rat femors
Acta Cirurgica Brasileira, 2006
PURPOSE:To investigate the influence of 650 nm GaAlAs laser on the action of bone morphogenetic protein (BMP) in bone defects produced in rat femurs. METHODS: The sample consisted of 12 male albino Wistar rats (Rattus norvegicus). The animals were randomly divided into four experimental groups. After undergoing anesthesia, the fur was removed from the lateral face of the right thigh and surgical dissection was performed to view the femur region. A bone defect was created using a spherical diamond-tipped drill bit. In groups 1 and 2, the defect was filled with a paste of Gen-Tech bone-inducing substance. The animals were treated with GaAlAs laser, at a predetermined dose of joules/cm² for 80 seconds, over an area of 1 cm². Groups 2 and 4 were used as controls. Bone samples were removed to perform histological procedures and morphometric analyses on the 7th, 14th and 21st days after the operation. The results obtained were subjected to statistical analysis using ANOVA variance accordi...