Evaluation of the Automated Haematology Analyser Sysmex NE-8000 (original) (raw)
Related papers
2014
Introduction: The Sysmex ® XE-2100D is a multiparameter hematology analyzer designed for hematology testing in samples with ethylenediamine tetraacetic acid (EDTA). Objectives: Considering the importance of this hematology analyzer for clinical and laboratory practice, the objective of this study was to evaluate its analytical performance, comparing the obtained results with quality specifications described in literature. Material and method: In the evaluation of analytical performance, according to recommendations of the document H26-A2 of the Clinical and Laboratory Standards Institute (CLSI), intra-run imprecision, inter-run imprecision, linearity, carryover, autosampler evaluation, clinical sensitivity of the atypical lymphocytes flag (n = 400 samples) were included, as well as the comparison between automated and manual leukocyte differential count (n = 400 samples), based on an adaptation of the document H20-A2 of CLSI. Results: Repeatability, reproducibility, linearity and carryover were satisfactory according to the manufacturer's specifications. The clinical sensitivity of the atypical lymphocytes flag showed efficiency, sensitivity and specificity of 92.5%, 65.2% and 94.1% respectively. The correlation coefficients between the automated and manual differential counts of neutrophils, lymphocytes, monocytes, eosinophils and basophils were 0.991, 0.99, 0.872, 0.974 and 0.557, respectively. Conclusions: The results were in accordance with quality specifications described in literature, indicating reliability in Sysmex ® XE-2100D. This fact ensures certainty to both laboratory professionals and medical staff. We conclude that the Sysmex ® XE-2100D showed excellent analytical performance, and is useful to provide reliable hematology data.
American journal of clinical pathology, 1993
An evaluation of SYSMEX NE-8000 (Toa Medical Electronics Co., Ltd., Kobe, Japan) hematology analyzer, including its automated five-part white blood cell (WBC) differential count, was performed in a high-volume outpatient laboratory. Precision, mixing studies, stability, carry-over, and linearity were all within the limits stated by the manufacturer. Evaluation of the five-part WBC differential revealed excellent correlation with manual differential neutrophil and lymphocyte counts. Monocyte, eosinophil, and basophil data were acceptable given the known poor precision of 200 cell manual WBC differential counts when low percentages of individual cell types are present. Overall, flagging for morphologic abnormalities displayed a sensitivity of 87% and a specificity of 97%. The combination of the flagging system with the visual record provided by the NE-8000 WBC histogram represents an effective tool for separating normal from abnormal specimens, and allows considerable selectivity in t...
An Evaluation of the Sysmex NE-8000® Hematology Analyzer
American Journal of Clinical Pathology
The Sysmex NE-8000® is a new, fully automated hematology analyzer capable of providing a five-part white blood cell differential count and identifying abnormal specimens. This instrument was evaluated on 5,000 consecutive blood specimens and compared to the Coulter S Plus-IV® analyzer and manual differential cell counts to determine the efficacy of its five-cell differential and screening capabilities. There was a high correlation between the commercial counters for the standard parameters, white blood cell count, red blood cell count, hemoglobin level, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, and platelet count (r > 0.95), except for the mean corpuscular hemoglobin concentration (r = 0.51), for which the NE-8000 was considered the more accurate measurement. Precision and linearity studies were excellent. The white blood cell count, red blood cell count, hemoglobin level, and platelet count were reproducible on specimens stored at 4°C or room temperature for 72 hours and the differential counts were reproducible for 12 hours. The correlations between automated and manual counts for neutrophils, eosinophils, basophils, and lymphocytes were excellent: r = 0.912, 0.945, 0.332, and 0.964, The Sysmex NE-8000® (Toa Medical Electronics, Kobe Japan) is a recently developed, high throughput automated hematology analyzer with the capacity to test 23 parameters at a maximal rate of 120 samples per hour in automatic mode. The instrument tests white blood cell count (WBC); red blood cell (RBC) count; hemoglobin concentration (HGB); hematocrit (HCT); mean corpuscular volume (MCV); mean corpuscular hemoglobin (MCH); mean corpuscular hemoglobin concentration (MCHC);
Evaluation of the leukocyte differential flags on an hematologic analyzer. The Cobas Argos 5 Diff
American journal of clinical pathology, 1995
To evaluate the leukocyte differential flags of the Cobas Argos 5 Diff., the authors performed a comparative study between their current analyzer, the Technicon H2, and the manual leukocyte differential. Samples (n = 1,600) were collected from the Blood Disease Department of their hospital and were tested on both Cobas Argos 5 Diff. (ABX/Roche Hematology Division, Montpellier, France) and Technicon H2. Abnormalities of the manual leukocyte differential (immature granulocytes, blast cells, atypical lymphocytes, hyperbasophil cells, erythroblasts, and hairy cells) were found in 597 samples. The authors determined the best cut-off of the quantitative flags--atypical lymphocytes (ALYs) and large immature cells (LICs)--using the likelihood ratio method, and the capability of the 5 Diff. qualitative flags to determine abnormal subpopulations by the predictive value of a positive result. The presence of particular combinations of flags was associated with band cells and blast cells of acut...
Indian Journal of Hematology and Blood Transfusion
Modern automated laboratory haematology analysers use various methods to measure different haematological parameters. These parameters are useful in the diagnostic and clinical interpretation of patient symptoms. So, it is very important to compare the performance of different analysers measuring the same parameter. Hence, a comparison of complete blood counts analysed by Sysmex XN 3000 and Horiba Yumizen H2500 was performed. Total 296 EDTA anti-coagulated blood samples were processed in both the analysers in duplicate within 4 h of collection. The white blood cell count, red blood cell count, erythrocyte indices, differential leukocyte count, platelet count and platelet indices and reticulocyte count were compared. A good level of correlation and agreement between different parameters were obtained. A strong correlation was observed (r > 0.9) between Sysmex XN 3000 and Yumizen H2500 for WBC (0.997), RBC (0.997), Haemoglobin (0.999), haematocrit (0.974), MCV (0.902), MCH (0.99),,...
Evaluation of the Vega Haematology Analyser in a university hospital setting
Clinical and Laboratory Haematology, 1999
The performance of the ABX Vega haematology analyser was compared with that of the Sysmex NE-8000, with specific attention to flagging performance and ergonomics. Eight hundred routine samples underwent precision and interinstrument variability studies and 168 samples corresponding to various blood disorders were studied meanwhile. Results from the two instruments gave excellent correlation (r × 0.900) for most parameters except MCHC (r = 0.114), basophil and monocyte percentages (r = 0.617 and 0.552, respectively). The reproducibility, repeatability, linearity, carry-over and stability of the Vega were satisfactory; 'flagging' occurred in 31% of routine samples with sensitivity 88.8%, specificity 41.3% and positive predictive value 85.7%. Various flags appeared in 91% (42/46) of cases where blast cells were microscopically identified. In the four remaining cases, CBC anomalies would themselves have justified microscopic examination of a smear. On 'CBC only' mode reagent consumption was significantly reduced. In the laboratory the analyser was best appreciated for its user-friendliness.
Performance evaluation of the Sysmex XS-1000 i automated haematology analyser
International Journal of Laboratory Hematology, 2009
Automated haematology analysers generate a complete blood count and five-part differential leucocyte count (CBC 5-diff) with a rapid turn around time. The Sysmex XS-1000i is a new, fully automated haematology analyser with 5-diff functionality. In our study, a Sysmex XS-1000i with auto-sampler was evaluated according to Clinical Laboratory Standards Institute (CLSI) and International Council for Standardization in Haematology (ICSH) guidelines. The purpose of our study was to evaluate performance characteristics of the Sysmex Evaluation of the instrument was carried out using K 2 EDTA (1.2-2 mg/mL blood; Sarstedt, Essen, Belgium) anticoagulated venous blood samples sent to our NPV, % (CI) 87 (78-94) PPV, % (CI) 56 (51-59) Efficiency, % (CI) 66 (60-70) CI, 95% confidence interval; NPV, negative predicitive value; PPV, positive predictive value.
Sysmex XN1000 versus Manual Method in Leukopenic Blood Samples
Journal of Clinical and Diagnostic Research, 2018
The first step in the assessment of hematologic function and diagnosis is the careful assessment of the blood elements. Leukocytes serve in immune function and include variety of sub-populations which have specific functions and characteristic morphologic appearances [1-3]. One of the first indicators of an underlying pathology is the change in the concentration of peripheral blood leukocytes. Presence of a disease is not ruled out by a normal total white blood cell (WBC) count, but leukopenia or leukocytosis provides an important clue to the disease process and deserves further investigation which includes leukocyte differential count to identify the concentration of different types of WBC's [4]. DLC comprises of five cell types namely neutrophils, lymphocytes, eosinophils, monocytes and basophils. Extended Differential Count (EDC) comprises of counting cells additional to the five leukocyte population e.g., IGs. EDC plays an important role in clinical and haematological analysis of several leukopathies [5]. Leukopenic samples pose a challenge for leukocyte differential count and require longer review times than samples having WBC count within the normal reference range or having elevated count [6]. Accurate and timely delivery of DLC reports by haematology laboratories is important and crucial in various clinical settings [7]. In the present era of multispecialty hospitals having high workload, there has arisen a necessity of reliable automated haematology analysers which can help to reduce slide review rate for manual differentials, aid in accurate reporting, standardise the process, increase efficacy of processing samples and reduce the TAT. Hence, the present study aims to compare DLC and IG flagging by new automated haematology analyser XN1000 with findings of manual method in leukopenic blood samples. MAtERIALS And MEthOdS A prospective hospital based study was carried out from November 2013 to June 2015 on 346 patients of both OPD and IPD, referred to the
American Journal of Clinical Pathology, 1992
The authors evaluated the performance of four modern, commercially available hematology analyzers for imprecision and inaccuracy in determining the leukocyte differential count. The evaluation was performed according to International Committee for Standardization in Haematology protocols and the National Committee for Clinical Laboratory Standards H20-T standard, using the same group of patients simultaneously. Imprecision was very low among all the analyzers for neutrophils and lymphocytes (coefficient of variation maximum = 4.12%), whereas for the other leukocyte populations it tended to increase as their presence percentage decreased. The imprecision of the analyzers was still lower than that of the microscopic method. The correlation with the manual 800 cell count (inaccuracy) was good for neutrophils, lymphocytes, and eosinophils (r = 0.974 to 0.888), less so for monocytes (r = 0.757 to 0.490), whereas it was poor for basophils (r = 0.532 to 0.078).