In vitro Spermatogenesis – Optimal Culture Conditions for Testicular Cell Survival, Germ Cell Differentiation, and Steroidogenesis in Rats (original) (raw)
2014, Frontiers in Endocrinology
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Complete Human and Rat Ex Vivo Spermatogenesis from Fresh or Frozen Testicular Tissue
Biology of reproduction, 2016
Until now, complete ex-vivo spermatogenesis has been reported only in the mouse. In this species the duration of spermatogenesis is 35 days, whereas it is 54 days in the rat and 74 days in the human. We performed long term (until 60 days) cultures of fresh or frozen rat or human seminiferous tubule segments in a bioreactor, made of a hollow cylinder of chitosan hydrogel. Testicular tissues were obtained from 8 or 20 day old male rats, or from adult human subjects having undergone hormonal treatments leading to a near complete regression of their spermatogenesis, before bilateral orchiectomy for gender reassignment. The progression of spermatogenesis was assessed by cytological analyses of the cultures; it was related with a dramatic increase in the levels of mRNAs specifically expressed by round spermatids: Transition protein 1, Transition protein 2 and Protamine 3 in rat cultures. Two to 3.8% of cells were found haploid cells by FISH analysis of human cultures. In this bioreactor, ...
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