Anti-Inflammatory Effects of the Methanol Extract of Sedum telephium ssp. maximum in Lipopolysaccharide- Stimulated Rat Peritoneal Macrophages (original) (raw)

Pharmacology, 2008

Abstract

Sedum telephium ssp. maximum is a medicinal plant that possesses anti-inflammatory, analgesic and keratolytic properties. We investigated the anti-inflammatory activity of its methanolic extract (STME) in rat peritoneal macrophages (MΦs) stimulated with lipopolysaccharide from Salmonella enteritidis. After stimulation with 10 µg/ml of LPS, MΦs were coincubated with different doses of STME (8, 16 and 32 µg/ml) or RPMI medium alone using different times of incubation. STME reduced levels of tumor necrosis factor-α, both mRNA and its protein, and significantly decreased IL-1β and IL-6 production. Moreover, STME inhibited inducible nitric oxide synthase expression and blunted nitrite release and inhibited both extracellular signal-regulated kinase 1/2 and c-Jun N-terminal kinase activation in lipopolysaccharide-stimulated MΦs. Data show that STME might be useful as a potential anti-inflammatory agent.

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