Antimicrobial efficacy of Kerr pulp canal sealer (EWT) in combination with 10% amoxicillin on Enterococcus faecalis: A confocal laser scanning microscopic study (original) (raw)
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Antimicrobial activity of endodontic sealers on Enterococcus faecalis
Journal of endodontics, 2003
Enterococcus faecalis has been shown to be highly resistant once established in the root canal system and may play an important role in endodontic failures. The purpose of this study was to evaluate the antimicrobial activity of four root canal sealers on E. faecalis. Seventeen blood-agar plates were inoculated with E. faecalis using the Lawn technique. Five discs were placed on each plate, four with sealers--Sealapex, Roth 811, Kerr EWT, and AH-Plus--and an ampicillin disc as the control. The plates were incubated at 37 degrees C. The zones of inhibition were measured at 24 and 48 h. Analysis using a one-way ANOVA and Tukey test showed a statistically significance difference (p < 0.05) between all four groups of sealers. Roth 811 showed the largest zone of inhibition (1.1 mm), followed by Sealapex (0.8 mm) and Kerr EWT (0.5 mm), whereas AH-Plus had no antimicrobial activity. There was no difference in the zones of inhibition between the 24- and 48-h time periods.
In vitro antibacterial activity of three root canal sealers against Enterococcus Faecalis
South African Dental Journal
The antibacterial activity of root canal sealers may contribute to the eradication of remaining bacteria in root canals following canal shaping and debridement. The aim of the study is to assess the antimicrobial effect of 3 endodontic sealers: Sealapex™, EndoREZ™ and Guttaflow bioseal™ against Enterococcus faecalis. This was a laboratory-based comparative study testing the antimicrobial activity of three endodontic sealers against Enterococcus faecalis. The endodontic sealers were tested unset 20 minutes after mixing and after setting. Testing after setting enabled the assessment of the antimicrobial activity of aged sealers after 7, 14, 21 and 28 days. A total of 150 samples were used for the study. The tested sealers were divided into 3 groups: • Group 1 (EndoREZ™) n = 45 plates, n = 5 control plates. • Group 2 (Guttaflow bioseal™) n = 45, n = 5 control plates. • Group 3 (Sealapex™) n = 45, n = 5 control plates. All the materials exhibited some activity against the bacteria. The ...
ISRN Dentistry, 2012
Aim. To evaluate the antibacterial activity of four endodontic sealers on Enterococcus faecalis by a direct contact test. Material and Methods. Enterococcus faecalis was used as a test organism. Direct contact test which is based on measuring the effect of close contact between test bacteria and tested material on the kinetics of bacterial growth was performed to overcome the disadvantages of agar diffusion test. The sealers tested were zinc oxide eugenol-based sealer, glass-ionomer-based sealer, polydimethyl-siloxane-based sealer, and urethane dimethacrylate resin-based sealer. Data was collected by recording the optical density with the help of a spectrophotometer. Results. The sealers exhibited different inhibitory effects. The results obtained were subjected to statistical analysis by Kruskal Wallis analysis of variance and Dunn's multiple comparison test. Group comparison showed very highly significant difference between the groups. Conclusion. Zinc oxide eugenol-based seal...
Journal of endodontics, 2006
The purpose of this in vitro study was to evaluate the antimicrobial effects of five antibiotics when added to Kerr Pulp Canal Sealer EWT against Enterococcus faecalis. Five antibiotics: amoxicillin, penicillin, clindamycin, metronidazole, and doxycycline, were added separately to Kerr sealer. Thirty brain heart infusion agar plates were inoculated with E. faecalis and sterile paper discs containing a sealer-antibiotic combination were randomly assigned to the inoculated plates. Kerr sealer on a sterile paper disc and a blank sterile paper disc served as the controls. Fifteen plates were incubated aerobically and the remaining 15 plates were incubated anaerobically at 37 degrees C. The zones of inhibition were measured at 48 hours. No significant differences (p > 0.05) were found between aerobic and anaerobic groups. The groups were combined and data analysis using a one-way ANOVA and Tukey's post hoc test was performed. Results revealed that sealer-antibiotic combinations co...
Antibacterial Properties of Four Endodontic Sealers
Journal of Endodontics, 2008
The purpose of this study was to evaluate the antimicrobial effects of root canal sealers. The direct contact test (DCT) was used to assess the antibacterial properties of AH plus, Apexit Plus, Epiphany SE, and Roe-koSeal when in contact with Enterococcus faecalis. The materials were examined immediately after setting and 1, 2, 7, and 14 days after aging in phosphate-bufferedsaline. Statistical analysis with two-way analysis of variance, one-way analysis of variance, and Tukey multiple comparison was applied to the data. Apexit Plus had a short-term antibacterial effect of 1 day on E. faecalis, whereas Epiphany SE enhanced bacterial growth for at least 7 days. AH plus and RoekoSeal were ineffective. (J Endod 2008;34:735-738)
In vitro evaluation of the antimicrobial activity of some endodontic sealers
Introduction: When performing endodontic treatment, the dentalsurgeon must find ways to eliminate or reduce the microorganismssituated in the root canal in cases of pulpal necrosis. Objective: Toanalyze the antimicrobial action of Endofill, Sealer 26, AHPlus andAcroseal endodontic sealers. Material and methods: Evaluation wasmade by Agar diffusion method in plates previously inoculated with thefollowing microorganisms: E. coli, E. faecalis, B. cereus and S. aureus. The zones of inhibition were measured after 48 hours of incubation at 37°C. Results: Acroseal sealer showed no antimicrobial activity against E. coli and E. faecalis and it showed moderate activity against B. cereus and S. aureus; AHPlus and Endofill showed high activity mainly against E. coli; Sealer 26 showed moderate activity against all of the microorganisms used. Conclusion: The majority of endodontic sealers have showed antimicrobial activity against the microorganisms used. AHPlus and Endofill sealers were the ones...
European Journal of Molecular & Clinical Medicine, 2021
Background: Irrigants and intracanal medicaments form a necessary adjunct that enhances the antimicrobial effect of mechanical cleansing and thus augments overall clinical efficacy. Hence; the present study was undertaken for analyzing and comparing the efficacy of three endodontic irrigating solutions against Enterococcus Faecalis. Materials & methods: A total of thirty single rooted mandibular first premolar specimens were included. Sectioning of the specimens was done at cement-enamel junction level. Instrumentation of the root canals was done with rotary instruments to an apical size of ProTaper F3. Vertical sectioning of all the specimens was done along the mid-sagittal plane into two halves. All the specimens were divided into following study groups: Group A: 5% Sodium hypochlorite, Group B: 0.2% Chitosan and Group C: Saline. Inoculation of the bacterium was done in the broth in all the tissue culture wells. The biofilm on root canal surface was taken with a sterile scalpel and inoculated on agar plates and incubated. Colony forming units were then analyzed. Analysis of all the results was done using SPSS software. Results: Mean CFU among specimens of group A, group B and group C were found to be 0.59, 2.118 and 90.184 respectively. While analyzing the results statistically, significant results were obtained. Conclusion: Sodium hypochlorite exhibited maximum efficacy against Enterococcus Faecalis.
2020
Introduction: For a successful endodontic treatment, antimicrobial property of endodontic filing is mandatory. Hence, the aim of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida albicans, and Staphylococcus aureus by direct contact test (DCT). Materials and Methods: The antimicrobial efficacy of three different sealers, AH Plus, mineral trioxide aggregate (MTA) Fillapex, and Endosequence BC was tested against E. faecalis ATCC 29212, C. albicans ATCC 10231 and S. aureus ATCC 25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension was drawn and spread over cultural plates to determine the colony count using a digital colony counter. Readings were taken at 1 h (fresh specimen), then at 24 h (after setting). The results were tabulated and statistical analysis was done using one way ANOVA and Tukey HSD test. Results: DCT showed a significant decre...
2010
Aim: The aim of this study was to assess the antibacterial activity of seven different root canal sealers used in primary teeth against Enterococcus faecalis. Material and Method: The study was conducted using the agar diffusion inhibitory test. The sealers used were Sure-paste, Metapex, Tg Pex, Calcicure, Endoflas FS, Sealapex, Ca(OH)2 mixed with %2 Chlorhexidine Gluconate solution and one control group material (Klorhex Oral Gel). The injectable form sealers and the freshly-mixed sealers were placed into the prepared wells on the agar plates which were inoculated with the Enterococcus faecalis. After incubation periods of 24 hours, 48 hours, and 72 hours, the zones of the growth inhibition were observed and measured. The average of 8 measurements for each material was taken. The data were compared using one-way analysis of variance (ANOVA) (p<0.05). Results: Even though Sure-paste, Calcicure and Ca(OH)2 powder mixed with Klorhex irrigation solution have shown antibacterial acti...