Sperm fatty acid composition, semen quality, and reproductive performance of roosters fed diets supplemented with n-3 fatty acids and ginger (Zingiber officinale) (original) (raw)
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Poultry Science, 2014
Exposure to high levels of polyunsaturated fatty acid predisposes spermatozoa to lipid peroxidation, resulting in their decreased fertility. Ginger powder (GP), which is high in antioxidative compounds, was fed to aged breeder roosters to improve their reproductive performance. Seventy-five 52-wk-old Cobb 500 breeder roosters randomly received either 0 (GP 0), 15 (GP 15), or 30 (GP 30) g of GP/kg of diet for 14 consecutive wk, during which time their seminal characteristics were evaluated every 2 wk. At the end of the trial, semen samples were tested for determination of sperm fatty acid (FA) concentration and seminal plasma total antioxidant capacity. Furthermore, sperm penetration was assayed, and using 225 artificially inseminated hens, fertility and hatchability rates were determined. Dietary GP improved sperm forward motility, live sperm percentage, and sperm plasma membrane integrity. These were associated with a decrease in the percentage of abnormal sperm. The seminal TBA reactive species concentration was lower in birds belonging to the GP 30 treatment in comparison with those in the GP 15 and GP 0 treatments. The feeding of GP resulted in overall decreases and increases in sperm saturated and unsaturated FA, respectively. The n-6:n-3 FA ratio of sperm was decreased in the GP 30 group in comparison with controls. The highest levels of sperm C20:4(n-6) and C22:6(n-3) FA were recorded in the GP 15 and GP 30 treatments, respectively. A higher percentage of sperm C22:4(n-6) FA was found in GP-fed roosters. Seminal plasma total antioxidant capacity was considerably improved by the GP 15 and GP 30 treatments. Further, a higher number of perivitelline membrane sperm penetration holes was recorded for the GP 30 treatment in comparison with the GP 15 and GP 0 treatments. Interestingly, although hatchability, chick quality, and embryonic mortality were not affected by dietary treatment, fertility rate was improved by the feeding of GP. In conclusion, dietary GP improved most of the seminal characteristics evaluated in aged roosters of this study, suggesting that it has potential for use in attenuating age-related subfertility in senescent male commercial broiler breeders.
Poultry Science, 2020
This study was conducted to evaluate the effects of dietary polyunsaturated fatty acids (PUFA) sources and rosemary leaves powder (RLP) on the semen quality, fatty acid analysis, and some reproductive hormones of senescent broiler breeder roosters. Thirty-five 45-wk-old Ross breeder roosters were randomly divided into 7 groups (5 birds/group), and received following treatments including control group (basal diet), fish oil (2%), corn oil (2%), an equal (50:50%) proportion of fish oil and corn oil (50:50%), fish oil (2%) with 5 g/kg capsulated RLP, corn oil (2%) with 5 g/kg capsulated RLP, and an equal (50:50) proportion of fish oil and corn oil (50:50%) with 5 g/kg capsulated RLP of diet for 60 D, during which time their seminal characteristics were evaluated every 20 D. At the end of the trial (on day 60), semen samples were tested for determination of sperm fatty acid analysis, lipid peroxidation, and some reproductive hormones. Results showed that feeding fish oil and fish/corn oil with RLP was associated with an increase in docosahexaenoic acid (C22:6n-3) and docosatetraenoic acid (C22:4n-6) in sperm. The fish oil diet increased the proportion of n-3 fatty acids in sperm, and as a consequence, the (n-6)/(n-3) ratio also decreased (P < 0.05). RLP (5 g/kg) to the fish and fish/corn-oil (50:50%)based diet resulted in improvement in sperm concentration, total motility (%), sperm progressive motility (%), membrane integrity, and viability in terms 0 to 60 day trial (P < 0.05). Diets and age interacted to positively affect sperm concentration and sperm membrane integrity. Also this herbal antioxidant decreased the seminal content of malondialdehyde (MDA) significantly (P < 0.05). Testosterone and LH serum levels of reproductive hormones were significantly higher in fish and fish/corn-oil with RPL (50:50%)-based diet than other groups (P < 0.05). It can be concluded that RLP as an antioxidant could remarkably improve the effects of n-3 and n-3/n-6 PUFA on sperm characteristics, seminal MDA, and hormones levels in aged breeder roosters. The susceptibility of semen to lipid peroxidation was increased in chickens fed without RLP. Future studies are needed to disclose the causal mechanisms involved.
Cell Journal, 2013
Objective: The effects of dietary fish oil on semen quality and sperm fatty acid (FA) profiles during consumption of n-3 FA as well as the persistency of FA in ram's sperm after removing dietary oil from the diet were investigated. Materials and Methods: Nine Zandi's rams were randomly assigned to two groups (isoenergetic and isonitrogenous diets): Control (CTR; n=5) and Fish oil (FO; n=4) for 70 days with a constant level of vitamin E in both groups. Semen was collected at the first week and at the end week of feeding period (phase 1). After feeding period, all rams were fed a conventional diet and semen samples were collected one and two months after removing FO (phase 2). The sperm parameters and FA profiles were measured by computer assisted semen analyzer (CASA) and gas chromatography (GC), respectively. The completely randomized design was used and data were analyzed using the SPSS 16. Does the conventional diet contain any fats/lipids? If so, what is the concentration of these fats given to the control group compared to treatement group? No, it dosnt. This diet typically have 2.5-3 % ether extract or crude fat originated from forages and some garin such as barley. Results: Dietary FO had significant positive effects on all sperm quality and quantity parameters compared with CTR during feeding period (P<0.05). The positive effects of FO on sperm concentration and total sperm output were observed one and two months after removing FO (P<0.05), whereas, other sperm parameters were unaffected. Before feeding, C14 (Myristic acid), C16 (Palmitic acid), C18 (Stearic acid), C18:1 (Oleic acid) and C22:6 Cell Journal (Yakhteh
Cell journal, 2014
The effects of dietary fish oil on semen quality and sperm fatty acid profiles during consumption of n-3 fatty acids as well as the persistency of fatty acids in ram's sperm after removing dietary oil from the diet were investigated. In this experimental study, we randomly assigned 9 Zandi rams to two groups (isoenergetic and isonitrogenous diets): control (CTR; n=5) and fish oil (FO; n=4) for 70 days with a constant level of vitamin E in both groups. Semen was collected at the first week and at the last week of the feeding period (phase 1). After the feeding period, all rams were fed a conventional diet and semen samples were collected one and two months after removal of FO (phase 2). The sperm parameters and fatty acid profiles were measured by computer assisted semen analyzer (CASA) and gas chromatography (GC), respectively. The completely randomized design was used and data were analyzed with SPSS version 16. Dietary FO had significant positive effects on all sperm quality a...
Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm
Animal, 2010
The fatty acid composition of sperm affects the fertilization rate. The objective was to investigate the effects of dietary fish oil (as a source of n-3 fatty acids) on semen quality and sperm fatty acid composition in sheep. Eight Zandi fat-tailed rams were randomly allocated into two groups and fed either a control diet or a diet supplemented with fish oil. Both diets were isocaloric and isonitrogenous and were fed for 13 weeks, starting in the middle of the breeding season. Semen samples were collected weekly and their characteristics evaluated by standard methods, whereas samples collected at the start and end of the study were assessed (gas chromatography) for sperm lipid composition. Mean (±s.e.m.) sperm concentrations (4.3 × 109± 1.3 × 108v. 3.9 × 109± 1.3 × 108sperm/ml and percentages of motile (77.25 ± 3.34v. 60.8 ± 3.34) and progressively motile sperm (74.13 ± 1.69v. 62.69 ± 1.69) were significantly higher in the fish oil group than control. Dietary fish oil increased the ...
Dietary fish and evening primrose oil with vitamin E effects on semen variables in cockerels
British Poultry Science, 2005
1. Our aim was to determine the effect of n-3 (2%, wt/wt, fish oil rich diet) and n-6 (2%, wt/wt, evening primrose oil rich diet) fatty acid dietary supplementation and their combination with two concentrations of vitamin E (40 vs 200 mg/kg) on semen variables and on fatty acid and vitamin E profiles of spermatozoa in broiler breeders at 32, 42 and 52 weeks of age. 2. The inclusion of fish oil in the cockerel diets increased the docosahexaenoic acid proportion in the sperm phospholipid fraction, which was almost threefold higher compared to the other two groups irrespective of vitamin E supplementation. 3. In contrast, an increase in the proportion of total n-6 polyunsaturates, mainly 22:4n-6, was observed in the evening primrose oil group compared to the control only when the dietary content of vitamin E was increased to 200 mg/kg. 4. Sperm concentration was decreased in the fish and evening primrose oil groups if vitamin E was 40 mg/kg, but such an effect was prevented in the fish, not the evening primrose oil group, by increasing the vitamin E to 200 mg. 5. The proportion of motile spermatozoa was improved by the increased supplementation of vitamin E in all oil treatments.
Effects of Inclusion of Polyunsaturated Fatty Acids and Antioxidants on Spermatozoa
2015
This study was conducted to determine the effects of increasing dietary polyunsaturated fatty ac-ids (PUFA) and antioxidants (Se and vitamin E) on spermatozoa morphology. At 24 weeks of age, Potchefstroom Koekoek cockerels (n = 60) were selected from the base population. Cockerels were randomly allocated into five dietary treatments with 12 cockerels per dietary treatment. The diets consisted of commercial layer diet (CL), commercial cockerel diet (CC), modified layer diet (ML), modified layer diet +PUFA (MLP), and modified layer +PUFA+ antioxidants (MLPA) diet. At 36 weeks of age, six cockerels with ≥ 75 % sperm motility rate following analysis by Computer Aided Sperm Analysis (CASA) were selected from the base population in each dietary treatment group. Semen volume, sperm cells concentration and dead cells did not differ significantly among any of the groups. However, live and normal spermatozoa were significantly higher in the MLPA group. The bent mid-piece, bent mid-piece plus ...
Effect of dietary ellagic acid supplementation on semen quality parameters in chickens
Ellagic acid (EA), a polyphenoloic constituent in fruits and nuts, has been shown to have antioxidant and antiapoptotic properties. The aim of this study was to investigate the effect of dietary EA supplementation on semen quality parameters in chickens. Forty roosters (Dahlem Red breed), 28 weeks of age, were randomly assigned to four groups and fed diets containing EA at 0, 25, 50, or 75 mg/kg for 8 weeks. Semen was evaluated for volume, appearance, individual motility, sperm concentration, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) dye reduction test, and live, dead and abnormal sperm counts. The seminal plasma was separated and evaluated for protein, alkaline phosphatase, and lipid peroxidation. At the end of the experiment, blood from individual birds was collected, and serum was separated and evaluated for superoxide dismutase activity, lipid peroxidation, and ferric-reducing ability of plasma. Supplementation with EA had no effect (P > 0.05) on gross semen parameters. Seminal plasma protein and lipid peroxidation were different (P<0.01) between the treatment groups. The group fedEAat 75 mg/kg had high (P<0.01) seminal plasma lipid peroxidation and lower (P < 0.05) serum superoxide dismutase activity. In conclusion, dietary supplementation of EA in chickens had no effect on the gross semen qualities and increased lipid peroxidation in seminal plasma.
Indian Journal of Animal Sciences, 2020
The objective of the present study was to evaluate the efficacy of dietary supplementation of omega-3 rich oils, chia seed oil (CSO; Salvia hispanica) and linseed oil (LSO; Linum usitatissimum), on seminal characteristics, and plasma testosterone levels in Beetal bucks. The bucks were fed diet supplemented with either LSO or CSO @1% on dry matter basis or un-supplemented diet for 100 days. Daily intake of omega-3 fatty acids was 0.60 (control diet), 4.04 (diet supplemented with LSO) and 4.57 g (diet supplemented with CSO). Ultrasonography revealed no significant improvement in the testicular size yet the growth rate was much high in animals fed omega-3 supplemented diet. Ejaculate volume, individual motility, viability, sperm concentration, plasma membrane and acrosome integrity were significantly higher in bucks fed CSO supplemented diet as compared to LSO and control diet. The percentage of abnormal spermatozoa was significantly less in bucks fed CSO and LSO diet than control. Melanoaldehyde (MDA, µmole/10 9 sperm) concentration was lowest in bucks fed CSO diet followed by LSO and control diet. Activity of superoxide dismutase, glutathione reductase and catalase was significantly higher in spermatozoa of bucks fed CSO and LSO diet as compared to control. Bucks fed CSO and LSO supplemented diet had nonsignificantly higher plasma testosterone concentrations than control. These findings indicated that CSO supplementation in the diets may be helpful in improving the process of spermatogenesis, sperm output, quality and fertility of bucks.