Simultaneous Estimation of Cefixime and Azithromycin in Api's and Pharmaceutical Dosage Form by RP-HPLC (original) (raw)

Abstract

A new combination of cefixime and azithromycin are used for the treatment of upper and lower respiratory tract infection. A simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of cefixime and azithromycin in pharmaceutical dosage form .The mobile phase consisted of 0.02M Potassium dihydrogen phosphate (KH 2 PO 4): Acetonitrile in the ratio of 65:35 (v/v). The method obeys Beer's law in the concentration range of 40-60 μg/ml (R 2 = 0.998) for cefixime and 50-70μg/ml (R 2 = 0.999) for azithromycin. The LOD and LOQ were found to be 1.06μg/ml and 3.21μg/ml for cefixime and 2.48μg/ml and 7.50μg/ml for azithromycin respectively. The recovery of cefixime and azithromycin were found to be 99.84% and 100.76% respectively showing accuracy of the method. The method was validated statistically as per ICH guidelines. The method showed good reproducibility and recovery with % RSD less than 2. So, the proposed method was found to be simp...

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