The interaction of atmospheric pressure plasma jets with cancer and normal cells: generation of intracellular reactive oxygen species and changes of the cell proliferation and cell cycle (original) (raw)
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The effects of cold atmospheric plasma jets on B16 and COLO320 tumoral cells
Roumanian archives of microbiology and immunology
Cold atmospheric plasma treatment acts at the cellular level to remove diseased tissue without inflammation and damage, to suppress infections and to modulate the viability (apoptosis/necrosis) of tumoral cells. It is also known that, a major cause of anti-tumor chemotherapy failure is the development of multidrug resistance (MDR) of tumors. This study reveals the effect of high voltage pulsed, repetitive cold atmospheric plasma jets which are chemically activated with oxygen, on B16 tumoral cells (murine melanoma cell line) and COLO320DM multidrug resistant cells (human colon cancer cell line). The tests have been performed on human colon cancer cell line COLO320DM and murine melanoma cell line B16-F10. These cell lines have been treated with cold helium or helium-oxygen generated plasma jets and the consequent apoptosis has been analyzed by means of flow cytometric method. A treatment time-dependent apoptosis has been observed only in the case of 816-F10 cells interacting with hel...
Applied Physics Letters, 2012
Helium/oxygen atmospheric pressure plasma jets driven by pulsed dc voltage with repetition rate of several tens of kilohertz are utilized for plasma-cell interactions. The effect of operating parameters on the apoptosis of cultured human bladder (EJ) cancer cells is investigated. The parameters such as applied voltage, pulse repetition frequency, and duty ratio determine the plasma dose. The apoptotic changes in cells with plasma treatment are detected by staining assay and flow cytometry. Apoptosis rates are observed to correlate well with both the plasma dose and the levels of intracellular and extracellular reactive oxygen species. V
AIP Advances, 2013
Atmospheric pressure plasma jets employing nitrogen, helium, or argon gases driven by low-frequency (several tens of kilohertz) ac voltage and pulsed dc voltage were fabricated and characterized. The changes in discharge current, optical emission intensities from reactive radicals, gas temperature, and plume length of plasma jets with the control parameters were measured and compared. The control parameters include applied voltage, working gas, and gas flow rate. As an application to plasma-cancer cell interactions, the effects of atmospheric pressure plasma jet on the morphology and intracellular reactive oxygen species (ROS) level of human lung adenocarcinoma cell (A549) and human bladder cancer cell (EJ) were explored. The experimental results show that the plasma can effectively control the intracellular concentrations of ROS. Although there exist slight differences in the production of ROS, helium, argon, or nitrogen plasma jets are found to be useful in enhancing the intracellular ROS concentrations in cancer cells.
Applied Physics Letters, 2013
The effects of atmospheric pressure plasma jet on cancer cells (human lung carcinoma cells) and normal cells (embryonic kidney cells and bronchial epithelial cells) were investigated. Using a detection dye, the production of intracellular reactive oxygen species (ROS) was found to be increased in plasma-treated cells compared to non-treated and gas flow-treated cells. A significant overproduction of ROS and a reduction in cell viability were induced by plasma exposure on cancer cells. Normal cells were observed to be less affected by the plasma-mediated ROS, and cell viability was less changed. The selective effect on cancer and normal cells provides a promising prospect of cold plasma as a cancer therapy. V
Effect of non-thermal atmospheric pressure plasma jet on human breast cancer cells
2012
The aim of this paper is to investigate the effect of nonthermal atmospheric pressure plasma jet on human breast cancer and normal cells. High voltage, dc-pulsed power supply was used to generate low temperature helium plasma in atmospheric pressure. In this paper, cancer and normal cells were exposed to plasma during four time steps. For further exploration, Doxorubicin as common chemotherapy drug was employed to compare the efficacy of plasma treatment with convectional cancer therapy. Also, the Caspase-Glo 3/7 assay was obtained to reveal the death process of cancer and normal cells. The results of MTT and apoptosis assay showed that the plasma treatment has drastically reduced the viability of breast cancer cells, while it has no significant damage to the normal cells. In addition, after plasma treatment three different zones were formed in the plate, which will represent the detachment of cells from plate surface. Moreover, adding 5% oxygen to the helium plasma will lead to enhancement of cancer cells viability reduction. In addition, the outcome of this paper has verified that the plasma treatment successfully overcomes drug treatment in inhibition of the cancer cells viability, while decreases the adverse effect of drug treatment.
Cold atmospheric plasma jet-generated RONS and their selective effects on normal and carcinoma cells
Scientific reports, 2016
Cold atmospheric helium plasma jets were fabricated and utilized for plasma-cell interactions. The effect of operating parameters and jet design on the generation of specific reactive oxygen and nitrogen species (RONS) within cells and cellular response were investigated. It was found that plasma treatment induced the overproduction of RONS in various cancer cell lines selectively. The plasma under a relatively low applied voltage induced the detachment of cells, a reduction in cell viability, and apoptosis, while the plasma under higher applied voltage led to cellular necrosis in our case. To determine whether plasma-induced reactive oxygen species (ROS) generation occurs through interfering with mitochondria-related cellular response, we examined the plasma effects on ROS generation in both parental A549 cells and A549 ρ(0) cells. It was observed that cancer cells were more susceptible to plasma-induced RONS (especially nitric oxide (NO) and nitrogen dioxide (NO2(-)) radicals) tha...
Induction of apoptosis in human breast cancer cells by a pulsed atmospheric pressure plasma jet
Applied Physics Letters, 2010
By using an atmospheric pressure plasma jet driven by pulsed dc voltage with repetition rate of several tens of kilohertz, we were able to induce apoptosis in cultured human breast cancer cells ͑MCF-7͒. The apoptotic changes in cells with plasma treatment were detected by flow cytometry and fluorescence staining assay. A significant portion of these cells was observed to exhibit the apoptotic fragmentation. Helium plasma with additive O 2 gas was found to be effective in the induction of apoptosis. This plasma jet provides an effective mode of human breast cancer cell therapy.
PLoS ONE, 2014
The plasma jet has been proposed as a novel therapeutic method for cancer. Anticancer activity of plasma has been reported to involve mitochondrial dysfunction. However, what constituents generated by plasma is linked to this anticancer process and its mechanism of action remain unclear. Here, we report that the therapeutic effects of air plasma result from generation of reactive oxygen/nitrogen species (ROS/RNS) including H 2 O 2 , Ox, OH 2 , NO 2, NOx, leading to depolarization of mitochondrial membrane potential and mitochondrial ROS accumulation. Simultaneously, ROS/RNS activate c-Jun NH 2terminal kinase (JNK) and p38 kinase. As a consequence, treatment with air plasma jets induces apoptotic death in human cervical cancer HeLa cells. Pretreatment of the cells with antioxidants, JNK and p38 inhibitors, or JNK and p38 siRNA abrogates the depolarization of mitochondrial membrane potential and impairs the air plasma-induced apoptotic cell death, suggesting that the ROS/RNS generated by plasma trigger signaling pathways involving JNK and p38 and promote mitochondrial perturbation, leading to apoptosis. Therefore, administration of air plasma may be a feasible strategy to eliminate cancer cells.