Dental follicle mesenchymal stem cells down‐regulate Th2‐mediated immune response in asthmatic patients mononuclear cells (original) (raw)

SummaryBackgroundAsthma is a chronic inflammatory disease in which inflammatory responses have the polarisation of CD4+ T cells to Th2 cells. Dental follicle mesenchymal stem cells (DFSCs) have strong anti‐inflammatory properties comparable to other mesenchymal stem cells.ObjectiveWe investigated the immunomodulatory effects of DFSCs on CD4+ T helper cell responses of asthmatic patients and compared the results with those obtained with asthmatic subjects on immunotherapy and with healthy individuals.MethodPeripheral blood mononuclear cells (PBMC) were isolated from immunotherapy naïve asthmatics, asthmatics on subcutaneous Der p1 immunotherapy and from healthy individuals. PBMC were pre‐conditioned with anti‐CD3/anti‐CD28 mAbs, Der p1 or IFN‐γ in the presence and absence of DFSCs and analysed for T cell viability and proliferation, CD4+CD25+FOXP3+ regulatory T cell frequencies, cytokine expression, and GATA3, T bet and FoxP3 expressions. Neutralisation of TGF‐β and blockade of IDO a...